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Gene Review

CHO2  -  phosphatidylethanolamine N-methyltransferase

Saccharomyces cerevisiae S288c

Synonyms: Choline-requiring protein 2, G6673, PEAMT, PEM1, Phosphatidylethanolamine N-methyltransferase, ...
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High impact information on CHO2


Biological context of CHO2


Anatomical context of CHO2

  • Membranes of cho2 mutant cells grown in defined medium contain approximately 10% phosphatidylcholine (PC) and 40-50% PE as compared to wild-type levels of 40-45% PC and 15-20% PE [11].

Associations of CHO2 with chemical compounds


Regulatory relationships of CHO2

  • When present in multiple copies the OPI3 gene efficiently suppresses the phospholipid methylation defect of a cho2 mutation [12].

Other interactions of CHO2

  • Sequence analysis established that the cDNA was not homologous to OPI3 or to CHO2, the only other yeast phospholipid N-methyltransferase, but was similar to several other classes of methyltransferases [13].
  • A strain carrying the tightest of the three alleles was examined in detail and was found to express the set of co-regulated phospholipid structural genes (INO1, CHO1, CHO2 and OP13) constitutively [14].
  • In order to investigate this regulation, we transformed wild-type yeast with a PEM1 promoter-lacZ fusion and isolated two mutants, named ric1 and ric2 (regulation by myo-inositol and choline), exhibiting decreased PEM1 expression [15].
  • The majority of potential UASINO elements from the INO1 promoter were found to be inactive, whereas all of the elements from the CHO2 promoter tested were active [7].
  • PS synthase expression was not depressed, while CDP-DG synthase and PI synthase expression decreased in cho2 and cho1 cells in the absence of inositol [16].

Analytical, diagnostic and therapeutic context of CHO2


  1. Inositol regulates phosphatidylglycerolphosphate synthase expression in Saccharomyces cerevisiae. Greenberg, M.L., Hubbell, S., Lam, C. Mol. Cell. Biol. (1988) [Pubmed]
  2. Expression of the Saccharomyces cerevisiae inositol-1-phosphate synthase (INO1) gene is regulated by factors that affect phospholipid synthesis. Hirsch, J.P., Henry, S.A. Mol. Cell. Biol. (1986) [Pubmed]
  3. The yeast phospholipid N-methyltransferases catalyzing the synthesis of phosphatidylcholine preferentially convert di-C16:1 substrates both in vivo and in vitro. Boumann, H.A., Chin, P.T., Heck, A.J., De Kruijff, B., De Kroon, A.I. J. Biol. Chem. (2004) [Pubmed]
  4. Cloning and expression of a novel phosphatidylethanolamine N-methyltransferase. A specific biochemical and cytological marker for a unique membrane fraction in rat liver. Cui, Z., Vance, J.E., Chen, M.H., Voelker, D.R., Vance, D.E. J. Biol. Chem. (1993) [Pubmed]
  5. Purification, characterization and catalytic properties of human sterol 8-isomerase. Nes, W.D., Zhou, W., Dennis, A.L., Li, H., Jia, Z., Keith, R.A., Piser, T.M., Furlong, S.T. Biochem. J. (2002) [Pubmed]
  6. Regulation of phosphatidylethanolamine methyltransferase and phospholipid methyltransferase by phospholipid precursors in Saccharomyces cerevisiae. Gaynor, P.M., Gill, T., Toutenhoofd, S., Summers, E.F., McGraw, P., Homann, M.J., Henry, S.A., Carman, G.M. Biochim. Biophys. Acta (1991) [Pubmed]
  7. Functional characterization of the repeated UASINO element in the promoters of the INO1 and CHO2 genes of yeast. Koipally, J., Ashburner, B.P., Bachhawat, N., Gill, T., Hung, G., Henry, S.A., Lopes, J.M. Yeast (1996) [Pubmed]
  8. Characterization of the methyltransferases in the yeast phosphatidylethanolamine methylation pathway by selective gene disruption. Kodaki, T., Yamashita, S. Eur. J. Biochem. (1989) [Pubmed]
  9. Yeast phosphatidylethanolamine methylation pathway. Cloning and characterization of two distinct methyltransferase genes. Kodaki, T., Yamashita, S. J. Biol. Chem. (1987) [Pubmed]
  10. Genomic analysis of the Opi- phenotype. Hancock, L.C., Behta, R.P., Lopes, J.M. Genetics (2006) [Pubmed]
  11. Saccharomyces cerevisiae cho2 mutants are deficient in phospholipid methylation and cross-pathway regulation of inositol synthesis. Summers, E.F., Letts, V.A., McGraw, P., Henry, S.A. Genetics (1988) [Pubmed]
  12. Molecular cloning of the yeast OPI3 gene as a high copy number suppressor of the cho2 mutation. Preitschopf, W., Lückl, H., Summers, E., Henry, S.A., Paltauf, F., Kohlwein, S.D. Curr. Genet. (1993) [Pubmed]
  13. The isolation and characterization in yeast of a gene for Arabidopsis S-adenosylmethionine:phospho-ethanolamine N-methyltransferase. Bolognese, C.P., McGraw, P. Plant Physiol. (2000) [Pubmed]
  14. A pleiotropic phospholipid biosynthetic regulatory mutation in Saccharomyces cerevisiae is allelic to sin3 (sdi1, ume4, rpd1). Hudak, K.A., Lopes, J.M., Henry, S.A. Genetics (1994) [Pubmed]
  15. The SNF2/SWI2/GAM1/TYE3/RIC1 gene is involved in the coordinate regulation of phospholipid synthesis in Saccharomyces cerevisiae. Kodaki, T., Hosaka, K., Nikawa, J., Yamashita, S. J. Biochem. (1995) [Pubmed]
  16. Regulation of CDP-diacylglycerol synthesis and utilization by inositol and choline in Schizosaccharomyces pombe. Gaynor, P.M., Greenberg, M.L. J. Bacteriol. (1992) [Pubmed]
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