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Gene Review

INO1  -  inositol-3-phosphate synthase INO1

Saccharomyces cerevisiae S288c

Synonyms: IPS, Inositol-3-phosphate synthase, J0610, MI-1-P synthase, MIP synthase, ...
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Disease relevance of INO1


High impact information on INO1

  • All strains containing suppressor mutations in RPB1 and RPB2 have reduced transcription of the INO1 gene and an inositol requirement [6].
  • Our results show that Snf1 and the acetyltransferase Gcn5 function in an obligate sequence to enhance INO1 transcription by modifying histone H3 serine-10 and lysine-14 [7].
  • Here we examine the mechanisms surrounding pSer10 at INO1, and at GAL1, which herein is identified as a new regulatory target of Snf1/pSer10 [8].
  • However, the level of expression of the INO1 and CHO1 target genes correlated with the level of INO2 transcription [9].
  • Isolation of the yeast INO1 gene: located on an autonomously replicating plasmid, the gene is fully regulated [10].

Biological context of INO1

  • Saccharomyces cerevisiae ire15 mutant has a defect in the expression of INO1, showing the inositol auxotrophic phenotype [Nikawa, J. (1994) Gene 149, 367-372] [11].
  • These results reveal a Snf1-dependent connection between fatty acid production and phospholipid biosynthesis, identify Acc1 as a Snf1 target important for INO1 transcription, and suggest models in which metabolites that are generated or utilized during fatty acid biosynthesis can significantly influence gene expression in yeast [12].
  • The Ino2p.Ino4p complex binds to a fragment of the INO1 promoter containing two copies of the consensus binding site for the bHLH family of proteins [13].
  • However, the beta-galactosidase gene fused with the INO1 promoter region was expressed normally in the scs2Delta strain [14].
  • Whereas repressed INO1 localizes throughout the nucleoplasm, the gene is recruited to the nuclear periphery upon transcriptional activation [15].

Anatomical context of INO1


Associations of INO1 with chemical compounds


Physical interactions of INO1

  • The Saccharomyces cerevisiae Isw2p-Itc1p complex represses INO1 expression and maintains cell morphology [21].
  • Neither Ino2p nor Ino4p translated alone is capable of forming a complex with the INO1 promoter fragment [13].
  • Ino2p-dependent binding activity overlapped with a region of the INO1 promoter that contains two potential HLH consensus binding sites [22].

Regulatory relationships of INO1

  • However, analysis of rapamycin-treated cells suggests that Sit4 represses INO1 transcription through multiple mechanisms, only one of which may involve inhibition of TOR signaling [23].
  • Two distinct promoter elements, the upstream repression sequence (URS1) and the INO1 upstream activation sequence (UASINO) both were found to be involved in enabling SIN3 to repress INO1 expression [24].
  • We found that cells that underproduced RPO21 were unable to derepress fully the expression of a reporter gene under the control of the INO1 UAS [2].

Other interactions of INO1


Analytical, diagnostic and therapeutic context of INO1


  1. Analysis of sequences in the INO1 promoter that are involved in its regulation by phospholipid precursors. Lopes, J.M., Hirsch, J.P., Chorgo, P.A., Schulze, K.L., Henry, S.A. Nucleic Acids Res. (1991) [Pubmed]
  2. Underproduction of the largest subunit of RNA polymerase II causes temperature sensitivity, slow growth, and inositol auxotrophy in Saccharomyces cerevisiae. Archambault, J., Jansma, D.B., Friesen, J.D. Genetics (1996) [Pubmed]
  3. Reaching for mechanistic consensus across life kingdoms: structure and insights into catalysis of the myo-inositol-1-phosphate synthase (mIPS) from Archaeoglobus fulgidus. Stieglitz, K.A., Yang, H., Roberts, M.F., Stec, B. Biochemistry (2005) [Pubmed]
  4. sll1722, an unassigned open reading frame of Synechocystis PCC 6803, codes for L-myo-inositol 1-phosphate synthase. Chatterjee, A., Majee, M., Ghosh, S., Majumder, A.L. Planta (2004) [Pubmed]
  5. Effect of nutritive elements on the extracellular protein of different Bacillus strains, toxic to mosquito larvae. Rady, M.H., el-Deen, A.F. Journal of the Egyptian Society of Parasitology. (1991) [Pubmed]
  6. A suppressor of a HIS4 transcriptional defect encodes a protein with homology to the catalytic subunit of protein phosphatases. Arndt, K.T., Styles, C.A., Fink, G.R. Cell (1989) [Pubmed]
  7. Snf1--a histone kinase that works in concert with the histone acetyltransferase Gcn5 to regulate transcription. Lo, W.S., Duggan, L., Emre, N.C., Belotserkovskya, R., Lane, W.S., Shiekhattar, R., Berger, S.L. Science (2001) [Pubmed]
  8. Histone H3 phosphorylation can promote TBP recruitment through distinct promoter-specific mechanisms. Lo, W.S., Gamache, E.R., Henry, K.W., Yang, D., Pillus, L., Berger, S.L. EMBO J. (2005) [Pubmed]
  9. Regulation of yeast phospholipid biosynthetic gene expression in response to inositol involves two superimposed mechanisms. Ashburner, B.P., Lopes, J.M. Proc. Natl. Acad. Sci. U.S.A. (1995) [Pubmed]
  10. Isolation of the yeast INO1 gene: located on an autonomously replicating plasmid, the gene is fully regulated. Klig, L.S., Henry, S.A. Proc. Natl. Acad. Sci. U.S.A. (1984) [Pubmed]
  11. Cloning and sequence of the SCS2 gene, which can suppress the defect of INO1 expression in an inositol auxotrophic mutant of Saccharomyces cerevisiae. Nikawa, J., Murakami, A., Esumi, E., Hosaka, K. J. Biochem. (1995) [Pubmed]
  12. Inhibition of acetyl coenzyme A carboxylase activity restores expression of the INO1 gene in a snf1 mutant strain of Saccharomyces cerevisiae. Shirra, M.K., Patton-Vogt, J., Ulrich, A., Liuta-Tehlivets, O., Kohlwein, S.D., Henry, S.A., Arndt, K.M. Mol. Cell. Biol. (2001) [Pubmed]
  13. INO2 and INO4 gene products, positive regulators of phospholipid biosynthesis in Saccharomyces cerevisiae, form a complex that binds to the INO1 promoter. Ambroziak, J., Henry, S.A. J. Biol. Chem. (1994) [Pubmed]
  14. Role of the yeast VAP homolog, Scs2p, in INO1 expression and phospholipid metabolism. Kagiwada, S., Zen, R. J. Biochem. (2003) [Pubmed]
  15. Gene recruitment of the activated INO1 locus to the nuclear membrane. Brickner, J.H., Walter, P. PLoS Biol. (2004) [Pubmed]
  16. Regulation of phosphatidylinositol 4-kinase from the yeast Saccharomyces cerevisiae by CDP-diacylglycerol. Nickels, J.T., Buxeda, R.J., Carman, G.M. J. Biol. Chem. (1994) [Pubmed]
  17. The INO2 and INO4 loci of Saccharomyces cerevisiae are pleiotropic regulatory genes. Loewy, B.S., Henry, S.A. Mol. Cell. Biol. (1984) [Pubmed]
  18. A role for phospholipase D (Pld1p) in growth, secretion, and regulation of membrane lipid synthesis in yeast. Sreenivas, A., Patton-Vogt, J.L., Bruno, V., Griac, P., Henry, S.A. J. Biol. Chem. (1998) [Pubmed]
  19. Regulation of the yeast INO1 gene. The products of the INO2, INO4 and OPI1 regulatory genes are not required for repression in response to inositol. Graves, J.A., Henry, S.A. Genetics (2000) [Pubmed]
  20. Lithium and valproate decrease inositol mass and increase expression of the yeast INO1 and INO2 genes for inositol biosynthesis. Vaden, D.L., Ding, D., Peterson, B., Greenberg, M.L. J. Biol. Chem. (2001) [Pubmed]
  21. The Saccharomyces cerevisiae Isw2p-Itc1p complex represses INO1 expression and maintains cell morphology. Sugiyama, M., Nikawa, J. J. Bacteriol. (2001) [Pubmed]
  22. Functional characterization of the INO2 gene of Saccharomyces cerevisiae. A positive regulator of phospholipid biosynthesis. Nikoloff, D.M., Henry, S.A. J. Biol. Chem. (1994) [Pubmed]
  23. The Snf1 protein kinase and Sit4 protein phosphatase have opposing functions in regulating TATA-binding protein association with the Saccharomyces cerevisiae INO1 promoter. Shirra, M.K., Rogers, S.E., Alexander, D.E., Arndt, K.M. Genetics (2005) [Pubmed]
  24. SIN3 works through two different promoter elements to regulate INO1 gene expression in yeast. Slekar, K.H., Henry, S.A. Nucleic Acids Res. (1995) [Pubmed]
  25. Regulation of phospholipid biosynthetic enzymes by the level of CDP-diacylglycerol synthase activity. Shen, H., Dowhan, W. J. Biol. Chem. (1997) [Pubmed]
  26. The yeast UME6 gene is required for both negative and positive transcriptional regulation of phospholipid biosynthetic gene expression. Jackson, J.C., Lopes, J.M. Nucleic Acids Res. (1996) [Pubmed]
  27. Isolation and characterization of genes that promote the expression of inositol transporter gene ITR1 in Saccharomyces cerevisiae. Nikawa, J., Hosaka, K. Mol. Microbiol. (1995) [Pubmed]
  28. Evidence for the involvement of the Glc7-Reg1 phosphatase and the Snf1-Snf4 kinase in the regulation of INO1 transcription in Saccharomyces cerevisiae. Shirra, M.K., Arndt, K.M. Genetics (1999) [Pubmed]
  29. INO1-100: an allele of the Saccharomyces cerevisiae INO1 gene that is transcribed without the action of the positive factors encoded by the INO2, INO4, SWI1, SWI2 and SWI3 genes. Swift, S., McGraw, P. Nucleic Acids Res. (1995) [Pubmed]
  30. Interaction of trans and cis regulatory elements in the INO1 promoter of Saccharomyces cerevisiae. Lopes, J.M., Henry, S.A. Nucleic Acids Res. (1991) [Pubmed]
  31. Dimethyl sulfoxide exposure facilitates phospholipid biosynthesis and cellular membrane proliferation in yeast cells. Murata, Y., Watanabe, T., Sato, M., Momose, Y., Nakahara, T., Oka, S., Iwahashi, H. J. Biol. Chem. (2003) [Pubmed]
  32. L-myo-Inositol 1-phosphate synthase from Entamoeba histolytica. Lohia, A., Hait, N.C., Majumder, A.L. Mol. Biochem. Parasitol. (1999) [Pubmed]
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