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Gene Review

RAD14  -  Rad14p

Saccharomyces cerevisiae S288c

Synonyms: DNA repair protein RAD14, YM8325.02C, YMR201C
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Disease relevance of RAD14


High impact information on RAD14

  • Importantly, the abolishment of NER, by abrogation of RAD1 or RAD14, completely stopped repair of UV damage even during G2/M phase [3].
  • The post-UV histone modifications and chromatin remodeling at the repressed MFA2 promoter do not activate MFA2 transcriptionally, nor do they require damage recognition by Rad4p or Rad14p [4].
  • Rad1-Rad10 forms a ternary complex with the DNA damage recognition protein Rad14, providing a means for targeting this nuclease to the damage site [5].
  • Removal of cyclobutane pyrimidine dimers from damaged DNA by enzymatic photoreactivation has no effect on binding, strongly suggesting that RAD14 recognizes pyrimidine(6-4)pyrimidone photoproduct sites [6].
  • To examine this model further, we have constructed a histidine-tagged version of the yeast DNA damage recognition protein Rad14 [7].

Biological context of RAD14

  • To accomplish this, we have deleted one of the genes essential for NER in yeast, namely, RAD14, both in the context of an otherwise DNA repair-proficient strain (Deltarad14) and in a BER-defective isogenic derivative lacking the MAG1 gene (Deltamag1rad14) [2].
  • The second assay uses the apn1 apn2 rad14 triple mutant, which is viable but exhibits a spontaneous mutator phenotype [8].
  • Yeast DNA repair protein RAD23 promotes complex formation between transcription factor TFIIH and DNA damage recognition factor RAD14 [9].
  • The results also indicate that monoadduct formation by CDDP or 8-MOP at the doses used is not sufficient to delay S-phase in the rad14 Delta mutant [10].
  • S-phase delay was not observed after ICL damage introduced by cisplatin (CDDP) or 8-methoxypsoralen (8-MOP) during the G1-phase, in any of the above mutants, or in an isogenic rad14 Delta mutant deficient in nucleotide excision repair [10].

Anatomical context of RAD14

  • (4) Leaky alleles of rad1, rad3 and rad14 show a very marked difference in repair rates of the two lesions, rather like the human XPA revertant cell line XP129 and the Chinese hamster mutants UV61 and V-H1 [11].

Associations of RAD14 with chemical compounds


Other interactions of RAD14

  • Low-molecular-weight DNA was observed in rad14 cdc9 and rad16 cdc9 strains [15].
  • The RAD4 and RAD14 genes have a particular role in repair following exposure to those ethylating agents that preferentially alkylate oxygen, but not to those that preferentially ethylate nitrogen [16].
  • The rad23 mutation does not suppress the high UV sensitivity of completely NER-deficient rad1 or rad14 strains [17].
  • Our data indicate that DNA damage is not the main determinant for cell killing by photodynamic treatment and that the type of damage induced is apparently not subject to RAD14- or RAD52 controlled repair [18].
  • Complex formation with damage recognition protein Rad14 is essential for Saccharomyces cerevisiae Rad1-Rad10 nuclease to perform its function in nucleotide excision repair in vivo [19].

Analytical, diagnostic and therapeutic context of RAD14


  1. Yeast RAD14 and human xeroderma pigmentosum group A DNA-repair genes encode homologous proteins. Bankmann, M., Prakash, L., Prakash, S. Nature (1992) [Pubmed]
  2. Nucleotide excision repair defect influences lethality and mutagenicity induced by Me-lex, a sequence-selective N3-adenine methylating agent in the absence of base excision repair. Monti, P., Iannone, R., Campomenosi, P., Ciribilli, Y., Varadarajan, S., Shah, D., Menichini, P., Gold, B., Fronza, G. Biochemistry (2004) [Pubmed]
  3. Homologous recombination is involved in transcription-coupled repair of UV damage in Saccharomyces cerevisiae. Aboussekhra, A., Al-Sharif, I.S. EMBO J. (2005) [Pubmed]
  4. UV irradiation stimulates histone acetylation and chromatin remodeling at a repressed yeast locus. Yu, Y., Teng, Y., Liu, H., Reed, S.H., Waters, R. Proc. Natl. Acad. Sci. U.S.A. (2005) [Pubmed]
  5. Transcription factor TFIIH and DNA endonuclease Rad2 constitute yeast nucleotide excision repair factor 3: implications for nucleotide excision repair and Cockayne syndrome. Habraken, Y., Sung, P., Prakash, S., Prakash, L. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  6. Yeast DNA-repair gene RAD14 encodes a zinc metalloprotein with affinity for ultraviolet-damaged DNA. Guzder, S.N., Sung, P., Prakash, L., Prakash, S. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  7. Affinity purification and partial characterization of a yeast multiprotein complex for nucleotide excision repair using histidine-tagged Rad14 protein. Rodriguez, K., Talamantez, J., Huang, W., Reed, S.H., Wang, Z., Chen, L., Feaver, W.J., Friedberg, E.C., Tomkinson, A.E. J. Biol. Chem. (1998) [Pubmed]
  8. Use of yeast for detection of endogenous abasic lesions, their source, and their repair. Boiteux, S., Guillet, M. Meth. Enzymol. (2006) [Pubmed]
  9. Yeast DNA repair protein RAD23 promotes complex formation between transcription factor TFIIH and DNA damage recognition factor RAD14. Guzder, S.N., Bailly, V., Sung, P., Prakash, L., Prakash, S. J. Biol. Chem. (1995) [Pubmed]
  10. S. cerevisiae has three pathways for DNA interstrand crosslink repair. Grossmann, K.F., Ward, A.M., Matkovic, M.E., Folias, A.E., Moses, R.E. Mutat. Res. (2001) [Pubmed]
  11. Repair of 6-4 photoproducts and cyclobutane pyrimidine dimers in rad mutants of Saccharomyces cerevisiae. McCready, S. Mutat. Res. (1994) [Pubmed]
  12. Three additional genes involved in pyrimidine dimer removal in Saccharomyces cerevisiae: RAD7, RAD14 and MMS19. Prakash, L., Prakash, S. Mol. Gen. Genet. (1979) [Pubmed]
  13. Physical and functional interactions between nucleotide excision repair and DNA damage checkpoint. Giannattasio, M., Lazzaro, F., Longhese, M.P., Plevani, P., Muzi-Falconi, M. EMBO J. (2004) [Pubmed]
  14. Spontaneous mutation, oxidative DNA damage, and the roles of base and nucleotide excision repair in the yeast Saccharomyces cerevisiae. Scott, A.D., Neishabury, M., Jones, D.H., Reed, S.H., Boiteux, S., Waters, R. Yeast (1999) [Pubmed]
  15. Incision and postincision steps of pyrimidine dimer removal in excision-defective mutants of Saccharomyces cerevisiae. Wilcox, D.R., Prakash, L. J. Bacteriol. (1981) [Pubmed]
  16. A complex pattern of sensitivity to simple monofunctional alkylating agents exists amongst the rad mutants of Saccharomyces cerevisiae. Cooper, A.J., Waters, R. Mol. Gen. Genet. (1987) [Pubmed]
  17. Analysis of gene- and strand-specific repair in the moderately UV-sensitive Saccharomyces cerevisiae rad23 mutant. Verhage, R.A., Zeeman, A.M., Lombaerts, M., van de Putte, P., Brouwer, J. Mutat. Res. (1996) [Pubmed]
  18. Single strand breaks and mutagenesis in yeast induced by photodynamic treatment with chloroaluminum phthalocyanine. Paardekooper, M., De Bruijne, A.W., Van Gompel, A.E., Verhage, R.A., Averbeck, D., Dubbelman, T.M., Van den Broek, P.J. J. Photochem. Photobiol. B, Biol. (1997) [Pubmed]
  19. Complex formation with damage recognition protein Rad14 is essential for Saccharomyces cerevisiae Rad1-Rad10 nuclease to perform its function in nucleotide excision repair in vivo. Guzder, S.N., Sommers, C.H., Prakash, L., Prakash, S. Mol. Cell. Biol. (2006) [Pubmed]
  20. Nucleotide excision repair in yeast is mediated by sequential assembly of repair factors and not by a pre-assembled repairosome. Guzder, S.N., Sung, P., Prakash, L., Prakash, S. J. Biol. Chem. (1996) [Pubmed]
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