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Gene Review

CWP1  -  Cwp1p

Saccharomyces cerevisiae S288c

Synonyms: Cell wall protein CWP1, Glycoprotein GP40, YJU1, YKL096W, YKL443
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High impact information on CWP1

  • Promoter-exchange experiments showed that expression in S/G2 phase is necessary but not sufficient for the normal localization of Cwp1p [1].
  • Cells deficient in both Dcw1p and Dfg5p were round and large, had cell walls that contained an increased amount of chitin and secreted a major cell wall protein, Cwp1p, into the medium [2].
  • At a molecular level, we found that a considerable amount of Cwp1p became anchored through a novel type of linkage for glycosylphosphatidylinositol (GPI)-dependent cell wall proteins, namely an alkali-labile linkage to beta1,3-glucan [3].
  • A single gene, CWP1, was recovered as a multicopy suppressor of the spo1 null [4].
  • Finally, in yeast mutants with impaired cell wall structure, expression of both CWP1 and CWP2 was modified [5].

Biological context of CWP1


Anatomical context of CWP1

  • The N-terminal sequence of a 55-kDa mannoprotein corresponded with the product of ORF YKL096w, which we named CWP1 (cell wall protein 1) [8].

Associations of CWP1 with chemical compounds

  • Depletion of Cwp1p or Tip1p also caused increased sensitivities to Congo red and calcofluor white, but the effects were less pronounced than for cwp2 delta [8].
  • Disruption of FKS1 reduces the glucan content of the cell wall, increases chitin content and activates the expression of CWP1, which encodes a glycosylphosphatidylinositol (GPI)-dependent cell wall protein [9].
  • The beta-1,6-glucan moiety could be removed from Cwp1p and other cell wall proteins by cleaving phosphodiester bridges either enzymatically using phosphodiesterases or chemically using ice-cold aqueous hydrofluoric acid [10].

Other interactions of CWP1

  • Furthermore, Cwp1p and Tip1p were shown to carry a beta 1,6-glucose-containing side chain [8].
  • Interestingly, the defective incorporation of beta1,3-glucan in the cell wall was accompanied by an increase in chitin and mannan content in the cell wall, an enhanced expression of cell wall protein 1 (Cwp1p), and an increase in beta1,3-glucan synthase activity, probably caused by the induced expression of Fks2p [11].

Analytical, diagnostic and therapeutic context of CWP1


  1. Role of cell cycle-regulated expression in the localized incorporation of cell wall proteins in yeast. Smits, G.J., Schenkman, L.R., Brul, S., Pringle, J.R., Klis, F.M. Mol. Biol. Cell (2006) [Pubmed]
  2. Two homologous genes, DCW1 (YKL046c) and DFG5, are essential for cell growth and encode glycosylphosphatidylinositol (GPI)-anchored membrane proteins required for cell wall biogenesis in Saccharomyces cerevisiae. Kitagaki, H., Wu, H., Shimoi, H., Ito, K. Mol. Microbiol. (2002) [Pubmed]
  3. Low external pH induces HOG1-dependent changes in the organization of the Saccharomyces cerevisiae cell wall. Kapteyn, J.C., ter Riet, B., Vink, E., Blad, S., De Nobel, H., Van Den Ende, H., Klis, F.M. Mol. Microbiol. (2001) [Pubmed]
  4. Spo1, a phospholipase B homolog, is required for spindle pole body duplication during meiosis in Saccharomyces cerevisiae. Tevzadze, G.G., Swift, H., Esposito, R.E. Chromosoma (2000) [Pubmed]
  5. Specific cell wall proteins confer resistance to nisin upon yeast cells. Dielbandhoesing, S.K., Zhang, H., Caro, L.H., van der Vaart, J.M., Klis, F.M., Verrips, C.T., Brul, S. Appl. Environ. Microbiol. (1998) [Pubmed]
  6. Molecular cloning of CWP1: a gene encoding a Saccharomyces cerevisiae cell wall protein solubilized with Rarobacter faecitabidus protease I. Shimoi, H., Iimura, Y., Obata, T. J. Biochem. (1995) [Pubmed]
  7. Novel plant-GARP-like transcription factors in Giardia lamblia. Sun, C.H., Su, L.H., Gillin, F.D. Mol. Biochem. Parasitol. (2006) [Pubmed]
  8. Identification of three mannoproteins in the cell wall of Saccharomyces cerevisiae. van der Vaart, J.M., Caro, L.H., Chapman, J.W., Klis, F.M., Verrips, C.T. J. Bacteriol. (1995) [Pubmed]
  9. Up-regulation of genes encoding glycosylphosphatidylinositol (GPI)-attached proteins in response to cell wall damage caused by disruption of FKS1 in Saccharomyces cerevisiae. Terashima, H., Yabuki, N., Arisawa, M., Hamada, K., Kitada, K. Mol. Gen. Genet. (2000) [Pubmed]
  10. Retention of Saccharomyces cerevisiae cell wall proteins through a phosphodiester-linked beta-1,3-/beta-1,6-glucan heteropolymer. Kapteyn, J.C., Montijn, R.C., Vink, E., de la Cruz, J., Llobell, A., Douwes, J.E., Shimoi, H., Lipke, P.N., Klis, F.M. Glycobiology (1996) [Pubmed]
  11. Loss of the plasma membrane-bound protein Gas1p in Saccharomyces cerevisiae results in the release of beta1,3-glucan into the medium and induces a compensation mechanism to ensure cell wall integrity. Ram, A.F., Kapteyn, J.C., Montijn, R.C., Caro, L.H., Douwes, J.E., Baginsky, W., Mazur, P., van den Ende, H., Klis, F.M. J. Bacteriol. (1998) [Pubmed]
  12. Systematic identification in silico of covalently bound cell wall proteins and analysis of protein-polysaccharide linkages of the human pathogen Candida glabrata. Weig, M., Jänsch, L., Gross, U., De Koster, C.G., Klis, F.M., De Groot, P.W. Microbiology (Reading, Engl.) (2004) [Pubmed]
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