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Gene Review

TIP1  -  Tip1p

Saccharomyces cerevisiae S288c

Synonyms: Temperature shock-inducible protein 1, YBR0622, YBR067C
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Disease relevance of TIP1


High impact information on TIP1

  • Like SEC20, TIP1 is required for ER to Golgi transport and depletion of Tip1p results in accumulation of an extensive network of ER plus small transport vesicles [2].
  • We therefore propose that Sec20p and Tip1p act together as a functional unit in the ER to Golgi transport step [2].
  • Tip1p was found only in mother cells, whereas Cwp2p was incorporated in small-to-medium-sized buds [3].
  • Southern hybridization analysis demonstrated that there are several genes homologous to the TIP 1 gene on the yeast genome [4].
  • The TIP 1 gene encodes a protein of 210 amino acid residues with a molecular weight of 20,727, containing 20.0% alanine and 23.3% serine [4].

Biological context of TIP1


Anatomical context of TIP1

  • The TIP1 product interacts with Sec20p and is thus involved in transport from endoplasmic reticulum to Golgi [7].
  • Homology between the CWP1 protein and TIP1 family of cold shock proteins suggests that they belong to a new family of cell wall proteins [8].
  • The TIP 1 protein is thus likely to be secreted across the membrane and anchored on the outside surface of the plasma membrane [4].

Associations of TIP1 with chemical compounds

  • The glucan-binding sugar chain of Tip1p was released by hydrazinolysis and isolated [1].
  • On the other hand, Tip1p contained no glucosamine, which is a component of the GPI anchor [1].

Other interactions of TIP1

  • Furthermore, Cwp1p and Tip1p were shown to carry a beta 1,6-glucose-containing side chain [9].
  • A 80-kDa mannoprotein was identified as the product of the TIP1 gene, and a 180-kDa mannoprotein corresponded to the product of the ORF YKL444, which we named CWP2 [9].

Analytical, diagnostic and therapeutic context of TIP1

  • A fragment involved in the cold-shock induction of TIP1 was used as a probe in gel retardation assays to identify the cold shock-factor [10].
  • Northern blot hybridization analysis of a series of 5' end, 3' end and internal deletions has revealed that at least four different regions are involved in the regulation of the expression of TIP1, a cold shock-inducible gene of Saccharomyces cerevisiae [10].
  • Coimmunoprecipitation and immunofluorescence using Tip1p and Sec20p or its cytoplasmic domain showed that the two proteins physically interact to form a stable complex [2].


  1. Structure of the glucan-binding sugar chain of Tip1p, a cell wall protein of Saccharomyces cerevisiae. Fujii, T., Shimoi, H., Iimura, Y. Biochim. Biophys. Acta (1999) [Pubmed]
  2. The TIP1 gene of Saccharomyces cerevisiae encodes an 80 kDa cytoplasmic protein that interacts with the cytoplasmic domain of Sec20p. Sweet, D.J., Pelham, H.R. EMBO J. (1993) [Pubmed]
  3. Role of cell cycle-regulated expression in the localized incorporation of cell wall proteins in yeast. Smits, G.J., Schenkman, L.R., Brul, S., Pringle, J.R., Klis, F.M. Mol. Biol. Cell (2006) [Pubmed]
  4. TIP 1, a cold shock-inducible gene of Saccharomyces cerevisiae. Kondo, K., Inouye, M. J. Biol. Chem. (1991) [Pubmed]
  5. Cold-shock induction of a family of TIP1-related proteins associated with the membrane in Saccharomyces cerevisiae. Kowalski, L.R., Kondo, K., Inouye, M. Mol. Microbiol. (1995) [Pubmed]
  6. A novel esterase from Saccharomyces carlsbergensis, a possible function for the yeast TIP1 gene. Horsted, M.W., Dey, E.S., Holmberg, S., Kielland-Brandt, M.C. Yeast (1998) [Pubmed]
  7. The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames. Voet, M., Defoor, E., Verhasselt, P., Riles, L., Robben, J., Volckaert, G. Yeast (1997) [Pubmed]
  8. Molecular cloning of CWP1: a gene encoding a Saccharomyces cerevisiae cell wall protein solubilized with Rarobacter faecitabidus protease I. Shimoi, H., Iimura, Y., Obata, T. J. Biochem. (1995) [Pubmed]
  9. Identification of three mannoproteins in the cell wall of Saccharomyces cerevisiae. van der Vaart, J.M., Caro, L.H., Chapman, J.W., Klis, F.M., Verrips, C.T. J. Bacteriol. (1995) [Pubmed]
  10. Identification of cis- and trans-acting elements involved in the expression of cold shock-inducible TIP1 gene of yeast Saccharomyces cerevisiae. Muñoz-Dorado, J., Kondo, K., Inouye, M., Sone, H. Nucleic Acids Res. (1994) [Pubmed]
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