Disease relevance of TIP1

• One mol of the C-terminal peptide of Tip1p digested with Achromobacter protease I also contained 7.9 mol of glucose and 1 mol of ethanolamine [1].

High impact information on TIP1

• Like SEC20, TIP1 is required for ER to Golgi transport and depletion of Tip1p results in accumulation of an extensive network of ER plus small transport vesicles [2].
• We therefore propose that Sec20p and Tip1p act together as a functional unit in the ER to Golgi transport step [2].
• Tip1p was found only in mother cells, whereas Cwp2p was incorporated in small-to-medium-sized buds [3].
• Southern hybridization analysis demonstrated that there are several genes homologous to the TIP 1 gene on the yeast genome [4].
• The TIP 1 gene encodes a protein of 210 amino acid residues with a molecular weight of 20,727, containing 20.0% alanine and 23.3% serine [4].

Biological context of TIP1

• Cell fractionation analysis as well as studies using a yeast strain that is conditionally deficient in glycosylation demonstrate that TIP1 is a heavily modified membrane-associated protein [5].
• N-terminal sequence analysis of the esterase yielded a sequence which was identical to the deduced amino acid sequence of the S. cerevisiae TIP1 gene [6].

Anatomical context of TIP1

• The TIP1 product interacts with Sec20p and is thus involved in transport from endoplasmic reticulum to Golgi [7].
• Homology between the CWP1 protein and TIP1 family of cold shock proteins suggests that they belong to a new family of cell wall proteins [8].
• The TIP 1 protein is thus likely to be secreted across the membrane and anchored on the outside surface of the plasma membrane [4].

Associations of TIP1 with chemical compounds

• The glucan-binding sugar chain of Tip1p was released by hydrazinolysis and isolated [1].
• On the other hand, Tip1p contained no glucosamine, which is a component of the GPI anchor [1].

Other interactions of TIP1

• Furthermore, Cwp1p and Tip1p were shown to carry a beta 1,6-glucose-containing side chain [9].
• A 80-kDa mannoprotein was identified as the product of the TIP1 gene, and a 180-kDa mannoprotein corresponded to the product of the ORF YKL444, which we named CWP2 [9].

Analytical, diagnostic and therapeutic context of TIP1

• A fragment involved in the cold-shock induction of TIP1 was used as a probe in gel retardation assays to identify the cold shock-factor [10].
• Northern blot hybridization analysis of a series of 5' end, 3' end and internal deletions has revealed that at least four different regions are involved in the regulation of the expression of TIP1, a cold shock-inducible gene of Saccharomyces cerevisiae [10].
• Coimmunoprecipitation and immunofluorescence using Tip1p and Sec20p or its cytoplasmic domain showed that the two proteins physically interact to form a stable complex [2].

References