The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

RTS1  -  protein phosphatase 2A regulatory subunit...

Saccharomyces cerevisiae S288c

Synonyms: OR26.04, PP2A, B subunit, B' delta isoform, Protein RTS1, Protein SCS1, SCS1, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of RTS1


High impact information on RTS1

  • In the absence of Ipl1, Rts1 can initially bind to centromeric regions but disappears from these regions after anaphase I onset [3].
  • Glucose effects on V-ATPase assembly did not involve the Ras-cyclic AMP pathway, Snf1p, protein kinase C, or the general stress response protein Rts1p [4].
  • Strains in which RTS1 has been disrupted are temperature sensitive (ts) for growth, are hypersensitive to ethanol, are unable to grow with glycerol as their only carbon source, and accumulate at nonpermissive temperatures predominantly as large-budded cells with a 2N DNA content and a nondivided nucleus [1].
  • The Saccharomyces cerevisiae gene RTS1 encodes a protein homologous to a variable B-type regulatory subunit of the mammalian heterotrimeric serine/threonine protein phosphatase 2A (PP2A) [1].
  • However, CLB2 overexpression has no suppressive effects on other aspects of the rts1-null phenotype [1].

Biological context of RTS1

  • Molecular genetic analysis of Rts1p, a B' regulatory subunit of Saccharomyces cerevisiae protein phosphatase 2A [1].
  • This gene, SCS1 (suppressor of chaperonin sixty-1), encodes a 757-amino-acid protein of as yet unknown function which, nonetheless, has human, rice, and Caenorhabditis elegans homologs with high degrees (ca. 60%) of amino acid sequence identity [2].
  • SCS1 is not an essential gene, but SCS1-null strains do not grow above 37 degrees C and show some growth-related defects at 30 degrees C as well [2].
  • This cell cycle arrest can be overcome and partial suppression of the ts phenotype of rts1-null cells occurs if the gene CLB2, encoding a Cdc28 kinase-associated B-type cyclin, is expressed on a high-copy-number plasmid [1].
  • We found the following: the level of each subunit remained constant throughout the cell cycle; there is at least 10 times more of one of the regulatory subunits (Rts1p) than the other (Cdc55p); Tpd3p, the structural subunit, is limiting for both catalytic and regulatory subunit binding [5].

Associations of RTS1 with chemical compounds

  • Immunoprecipitated Rts1p was found associated with type 2A-specific protein phosphatase activity that is sensitive to 2 nM okadaic acid, but not to 100 nM phosphatase inhibitor-2, and to be phosphorylated in vivo [6].

Other interactions of RTS1

  • A selection for high-copy suppressors of a ROX3 temperature-sensitive allele resulted in the isolation of RTS1, encoding a protein with homology to the B' regulatory subunit of protein phosphatase 2A0 [7].
  • Deletion of RTS1 caused temperature and osmotic sensitivity and increased accumulation of CYC7 RNA under all conditions [7].
  • E4orf4 required CDC55 to elicit its effect, whereas RTS1 was dispensable [8].
  • Deletion of RTS1, encoding a regulatory subunit of protein phosphatase 2A, results in constitutive amino acid signaling via increased Stp1p processing [9].


  1. Molecular genetic analysis of Rts1p, a B' regulatory subunit of Saccharomyces cerevisiae protein phosphatase 2A. Shu, Y., Yang, H., Hallberg, E., Hallberg, R. Mol. Cell. Biol. (1997) [Pubmed]
  2. SCS1, a multicopy suppressor of hsp60-ts mutant alleles, does not encode a mitochondrially targeted protein. Shu, Y., Hallberg, R.L. Mol. Cell. Biol. (1995) [Pubmed]
  3. The Aurora kinase Ipl1 maintains the centromeric localization of PP2A to protect cohesin during meiosis. Yu, H.G., Koshland, D. J. Cell Biol. (2007) [Pubmed]
  4. Reversible association between the V1 and V0 domains of yeast vacuolar H+-ATPase is an unconventional glucose-induced effect. Parra, K.J., Kane, P.M. Mol. Cell. Biol. (1998) [Pubmed]
  5. Localization of Saccharomyces cerevisiae protein phosphatase 2A subunits throughout mitotic cell cycle. Gentry, M.S., Hallberg, R.L. Mol. Biol. Cell (2002) [Pubmed]
  6. Saccharomyces cerevisiae homologs of mammalian B and B' subunits of protein phosphatase 2A direct the enzyme to distinct cellular functions. Zhao, Y., Boguslawski, G., Zitomer, R.S., DePaoli-Roach, A.A. J. Biol. Chem. (1997) [Pubmed]
  7. Rox3 and Rts1 function in the global stress response pathway in baker's yeast. Evangelista, C.C., Rodriguez Torres, A.M., Limbach, M.P., Zitomer, R.S. Genetics (1996) [Pubmed]
  8. Toxicity of human adenovirus E4orf4 protein in Saccharomyces cerevisiae results from interactions with the Cdc55 regulatory B subunit of PP2A. Roopchand, D.E., Lee, J.M., Shahinian, S., Paquette, D., Bussey, H., Branton, P.E. Oncogene (2001) [Pubmed]
  9. Deletion of RTS1, encoding a regulatory subunit of protein phosphatase 2A, results in constitutive amino acid signaling via increased Stp1p processing. Eckert-Boulet, N., Larsson, K., Wu, B., Poulsen, P., Regenberg, B., Nielsen, J., Kielland-Brandt, M.C. Eukaryotic Cell (2006) [Pubmed]
WikiGenes - Universities