The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

MCM10  -  Mcm10p

Saccharomyces cerevisiae S288c

Synonyms: DNA43, Minichromosome maintenance protein 10, Protein DNA43, YIL150C
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on MCM10


Biological context of MCM10


Anatomical context of MCM10

  • Mcm10p is an abundant protein (approximately 4 x 10(4) copies per haploid cell) that is almost exclusively localized in the chromatin and/or nuclear matrix fractions during all phases of the cell cycle [6].

Physical interactions of MCM10

  • Mcm10p functionally interacts with components of the pre-replicative complex (Mcm2-Mcm7 complex and origin recognition complex) as well as the pre-initiation complex component (Cdc45p) suggesting that it may be a component of the pre-RC as well as the pre-IC [3].
  • Moreover, overexpressed Cdc17 that is normally subject to rapid degradation is stabilized by Mcm10 co-overexpression but not by co-overexpression of the B-subunit of pol-alpha, Pol12 [7].
  • A motif search revealed a match to the proliferating cell nuclear antigen (PCNA)-interacting protein (PIP) box in Mcm10 [8].

Regulatory relationships of MCM10

  • Furthermore, mutations in MCM10 inhibit the ability of GBD-SIR3 to restore silencing when tethered to a defective HMR-E [5].
  • Fission yeast Cdc23/Mcm10 functions after pre-replicative complex formation to promote Cdc45 chromatin binding [9].

Other interactions of MCM10

  • Consistent with a direct silencing function, Mcm10p shows a two-hybrid interaction with Sir2p and Sir3p that is destroyed by the mcm10-1 mutation and dependent on the C-terminal 108 amino acids [5].
  • We screened for mutations that are lethal in combination with mcm10-1 and obtained seven mutants named slm1-slm6 for synthetically lethal with mcm10 [3].
  • DNA sequence analysis suggested that DNA43 and DNA52 encode proteins of 59.6 and 80.6 kDa, respectively [10].
  • The high degree of evolutionary conservation of this domain implies that stabilizing Cdc17 may be a conserved function of Mcm10 [7].
  • A conserved Hsp10-like domain in Mcm10 is required to stabilize the catalytic subunit of DNA polymerase-alpha in budding yeast [7].

Analytical, diagnostic and therapeutic context of MCM10


  1. Mcm10 and the MCM2-7 complex interact to initiate DNA synthesis and to release replication factors from origins. Homesley, L., Lei, M., Kawasaki, Y., Sawyer, S., Christensen, T., Tye, B.K. Genes Dev. (2000) [Pubmed]
  2. Mcm10 regulates the stability and chromatin association of DNA polymerase-alpha. Ricke, R.M., Bielinsky, A.K. Mol. Cell (2004) [Pubmed]
  3. Budding yeast mcm10/dna43 mutant requires a novel repair pathway for viability. Araki, Y., Kawasaki, Y., Sasanuma, H., Tye, B.K., Sugino, A. Genes Cells (2003) [Pubmed]
  4. The Cdc23 (Mcm10) protein is required for the phosphorylation of minichromosome maintenance complex by the Dfp1-Hsk1 kinase. Lee, J.K., Seo, Y.S., Hurwitz, J. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  5. Mcm10 is required for the maintenance of transcriptional silencing in Saccharomyces cerevisiae. Liachko, I., Tye, B.K. Genetics (2005) [Pubmed]
  6. Interactions between Mcm10p and other replication factors are required for proper initiation and elongation of chromosomal DNA replication in Saccharomyces cerevisiae. Kawasaki, Y., Hiraga, S., Sugino, A. Genes Cells (2000) [Pubmed]
  7. A conserved Hsp10-like domain in Mcm10 is required to stabilize the catalytic subunit of DNA polymerase-alpha in budding yeast. Ricke, R.M., Bielinsky, A.K. J. Biol. Chem. (2006) [Pubmed]
  8. Interaction between PCNA and diubiquitinated Mcm10 is essential for cell growth in budding yeast. Das-Bradoo, S., Ricke, R.M., Bielinsky, A.K. Mol. Cell. Biol. (2006) [Pubmed]
  9. Fission yeast Cdc23/Mcm10 functions after pre-replicative complex formation to promote Cdc45 chromatin binding. Gregan, J., Lindner, K., Brimage, L., Franklin, R., Namdar, M., Hart, E.A., Aves, S.J., Kearsey, S.E. Mol. Biol. Cell (2003) [Pubmed]
  10. Genetic and molecular analysis of DNA43 and DNA52: two new cell-cycle genes in Saccharomyces cerevisiae. Solomon, N.A., Wright, M.B., Chang, S., Buckley, A.M., Dumas, L.B., Gaber, R.F. Yeast (1992) [Pubmed]
WikiGenes - Universities