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Gene Review

MOB1  -  Mob1p

Saccharomyces cerevisiae S288c

Synonyms: DBF2 kinase activator protein MOB1, MPS1 binder 1, Maintenance of ploidy protein MOB1, YIL106W
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High impact information on MOB1

  • We investigated the function of two S. cerevisiae proteins related to the MEN proteins Mob1p and Dbf2p kinase [1].
  • The simultaneous binding of Cdc14p and Mob1p to the SPB in early anaphase suggests that Cdc14p initially activates the MEN [2].
  • We propose that Mob1 promotes this activation process by enabling Cdc15 to phosphorylate the critical Ser-374 and Thr-544 phosphoacceptor sites of Dbf2 [3].
  • Saccharomyces cerevisiae Mob1p is required for cytokinesis and mitotic exit [4].
  • We found that the expression of MOB1 is also cell cycle regulated, its expression peaking slightly before that of DBF2 at the G2/M boundary [5].

Biological context of MOB1

  • MOB1, an essential yeast gene required for completion of mitosis and maintenance of ploidy [6].
  • Conditional alleles of MOB1 cause a late nuclear division arrest at restrictive temperature [6].
  • We also examined the subcellular distribution of Mob1p during the cell cycle and found that Mob1p first localized to the spindle pole bodies during mid-anaphase and then localized to a ring at the bud neck just before and during cytokinesis [4].
  • One mob1 strain, which maintains stable haploidy at both permissive and restrictive temperature, diploidizes at permissive temperature when combined with the mps1-1 mutation [6].
  • In contrast, overexpression of MOB1 suppressed phenotypes associated with a dbf2-deleted strain and suppressed the lethality associated with a dbf2 dbf20 double deletion [5].

Associations of MOB1 with chemical compounds


Physical interactions of MOB1


Regulatory relationships of MOB1

  • We also demonstrate that mob1p can be precipitated from cells in a complex with the septation regulating kinase sid2p [9].

Other interactions of MOB1

  • Mob1p contains no known structural motifs; however MOB1 is a member of a conserved gene family and shares sequence similarity with a nonessential yeast gene, MOB2 [6].
  • Mob1p is a phosphoprotein in vivo and a substrate for the Mps1p kinase in vitro [6].
  • These results are consistent with DBF2 acting through MOB1 and aiding in its function [5].
  • Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex [3].

Analytical, diagnostic and therapeutic context of MOB1


  1. The Saccharomyces cerevisiae Mob2p-Cbk1p kinase complex promotes polarized growth and acts with the mitotic exit network to facilitate daughter cell-specific localization of Ace2p transcription factor. Weiss, E.L., Kurischko, C., Zhang, C., Shokat, K., Drubin, D.G., Luca, F.C. J. Cell Biol. (2002) [Pubmed]
  2. Regulation of the Bfa1p-Bub2p complex at spindle pole bodies by the cell cycle phosphatase Cdc14p. Pereira, G., Manson, C., Grindlay, J., Schiebel, E. J. Cell Biol. (2002) [Pubmed]
  3. Protein kinase Cdc15 activates the Dbf2-Mob1 kinase complex. Mah, A.S., Jang, J., Deshaies, R.J. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  4. Saccharomyces cerevisiae Mob1p is required for cytokinesis and mitotic exit. Luca, F.C., Mody, M., Kurischko, C., Roof, D.M., Giddings, T.H., Winey, M. Mol. Cell. Biol. (2001) [Pubmed]
  5. DBF2 protein kinase binds to and acts through the cell cycle-regulated MOB1 protein. Komarnitsky, S.I., Chiang, Y.C., Luca, F.C., Chen, J., Toyn, J.H., Winey, M., Johnston, L.H., Denis, C.L. Mol. Cell. Biol. (1998) [Pubmed]
  6. MOB1, an essential yeast gene required for completion of mitosis and maintenance of ploidy. Luca, F.C., Winey, M. Mol. Biol. Cell (1998) [Pubmed]
  7. The mitotic exit network Mob1p-Dbf2p kinase complex localizes to the nucleus and regulates passenger protein localization. Stoepel, J., Ottey, M.A., Kurischko, C., Hieter, P., Luca, F.C. Mol. Biol. Cell (2005) [Pubmed]
  8. Substrate specificity analysis of protein kinase complex Dbf2-Mob1 by peptide library and proteome array screening. Mah, A.S., Elia, A.E., Devgan, G., Ptacek, J., Schutkowski, M., Snyder, M., Yaffe, M.B., Deshaies, R.J. BMC Biochem. (2005) [Pubmed]
  9. The S. pombe orthologue of the S. cerevisiae mob1 gene is essential and functions in signalling the onset of septum formation. Salimova, E., Sohrmann, M., Fournier, N., Simanis, V. J. Cell. Sci. (2000) [Pubmed]
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