High impact information on DAL7

• During this work a new gene (DAL7) was identified and found to be regulated in the manner expected for an allantoin pathway gene [1].
• The purpose of this work is to extend our understanding of these elements and their roles to inducible allantoin pathway genes using the DAL7 (encoding malate synthase) as a model [2].
• Overlapping positive and negative GATA factor binding sites mediate inducible DAL7 gene expression in Saccharomyces cerevisiae [2].
• Operation of the DAL7 UIS required functional DAL81 and DAL82 gene products [3].
• We have isolated a second gene (MLS1), which in addition to DAL7, encodes malate synthase from S. cerevisiae [4].

Biological context of DAL7

• We also demonstrate that transcriptional activation, mediated by the DAL5 and DAL7 upstream activation sequences, requires a functional GLN3 gene product [5].
• Nucleotide sequence analysis of a cDNA clone encoding malate synthase of castor bean (Ricinus communis) reveals homology to DAL7, a gene involved in allantoin degradation in Saccharomyces cerevisiae [6].
• Missing from the DAL1 5' flanking region were any sequences with significant homology to the DAL7 UIS element required for response to inducer [7].

Other interactions of DAL7

• Also present upstream from DAL2 is a mutant form of the upstream induction sequence required for response of DAL7 to induction [8].
• Missing from the DAL3 upstream region were any sequences related to those shown to be required for a DAL7 response to inducer, the UIS element [9].

References