The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

Links

 

Gene Review

ASC1  -  guanine nucleotide-binding protein subunit...

Saccharomyces cerevisiae S288c

Synonyms: ASU9, CPC2, Guanine nucleotide-binding protein subunit beta-like protein, NAD1, RACK1, ...
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

High impact information on ASC1

  • We also show that purified Asc1p-deficient ribosomes have increased translational activity compared to that of wild-type yeast ribosomes [1].
  • Further, we demonstrate that asc1Delta null strains have increased levels of specific proteins in vivo and that this molecular phenotype is complemented by either Asc1p or RACK1 [1].
  • Yeast Asc1p and mammalian RACK1 are functionally orthologous core 40S ribosomal proteins that repress gene expression [1].
  • Mutations that activate Gcn4p function, such as gcd7-201, cpc2, and deletion of the translational regulatory region of the GCN4 gene, also cause salt sensitivity [2].
  • RACK1 recruits activated protein kinase C to the ribosome, which leads to the stimulation of translation through the phosphorylation of initiation factor 6 and, potentially, of mRNA-associated proteins [3].
 

Biological context of ASC1

 

Anatomical context of ASC1

  • In addition, the fact that RACK1 associates with membrane-bound receptors indicates that it promotes the docking of ribosomes at sites where local translation is required, such as focal adhesions [3].
  • Asc1p, a WD40-domain containing adaptor protein, is required for the interaction of the RNA-binding protein Scp160p with polysomes [4].
 

Associations of ASC1 with chemical compounds

  • Here, we show that CPC2 transcription depends on the carbon source and is induced during utilization of the sugar glucose [5].
  • Increased amounts of the Fhl1p co-regulator Ifh1p induce CPC2 transcription even when ethanol is utilized [5].
  • Whereas Gpa2 promotes glucose signaling through elevated production of cAMP, Asc1 has opposing effects on these same processes [6].
 

Regulatory relationships of ASC1

  • Cpc2p seems to be part of an additional control of Gcn4p activity, independent of its translational regulation [7].
  • Loss of CPC2 gene function suppresses a deletion of the GCN2 gene encoding the general control sensor kinase, but not a deletion in the GCN4 gene [7].
  • Deletion of CPC2/ASC1 abolished amino acid starvation-induced adhesive growth and impaired basal expression of FLO11 and its activation upon starvation in haploid cells [8].
 

Other interactions of ASC1

  • The WD protein Cpc2p is required for repression of Gcn4 protein activity in yeast in the absence of amino-acid starvation [7].
  • Deletion of FHL1 reduces CPC2 transcription significantly in presence of glucose, but has no effect when the non-fermentable carbon source ethanol is provided [5].
  • We propose that the absence of functional Asc1p allows the growth of hap1-hem1- cells by reducing the efficiency of translation [9].
  • Two of these associated proteins, Mpt4p and Asc1p, were nearly stoichiometrically bound to the ribosome [10].
  • The interaction of Scp160p with ribosomes depends on Asc1p [4].
 

Analytical, diagnostic and therapeutic context of ASC1

  • Interestingly, RACK1 is also a constituent of the eukaryotic ribosome, and a recent cryo-electron microscopy study localized it to the head region of the 40S subunit in the vicinity of the messenger RNA (mRNA) exit channel [3].

References

  1. Yeast Asc1p and mammalian RACK1 are functionally orthologous core 40S ribosomal proteins that repress gene expression. Gerbasi, V.R., Weaver, C.M., Hill, S., Friedman, D.B., Link, A.J. Mol. Cell. Biol. (2004) [Pubmed]
  2. The protein kinase Gcn2p mediates sodium toxicity in yeast. Goossens, A., Dever, T.E., Pascual-Ahuir, A., Serrano, R. J. Biol. Chem. (2001) [Pubmed]
  3. Regulation of eukaryotic translation by the RACK1 protein: a platform for signalling molecules on the ribosome. Nilsson, J., Sengupta, J., Frank, J., Nissen, P. EMBO Rep. (2004) [Pubmed]
  4. Asc1p, a WD40-domain containing adaptor protein, is required for the interaction of the RNA-binding protein Scp160p with polysomes. Baum, S., Bittins, M., Frey, S., Seedorf, M. Biochem. J. (2004) [Pubmed]
  5. The yeast CPC2/ASC1 gene is regulated by the transcription factors Fhl1p and Ifh1p. Kleinschmidt, M., Schulz, R., Braus, G.H. Curr. Genet. (2006) [Pubmed]
  6. The RACK1 ortholog Asc1 functions as a G-protein beta subunit coupled to glucose responsiveness in yeast. Zeller, C.E., Parnell, S.C., Dohlman, H.G. J. Biol. Chem. (2007) [Pubmed]
  7. The WD protein Cpc2p is required for repression of Gcn4 protein activity in yeast in the absence of amino-acid starvation. Hoffmann, B., Mösch, H.U., Sattlegger, E., Barthelmess, I.B., Hinnebusch, A., Braus, G.H. Mol. Microbiol. (1999) [Pubmed]
  8. The Saccharomyces homolog of mammalian RACK1, Cpc2/Asc1p, is required for FLO11-dependent adhesive growth and dimorphism. Valerius, O., Kleinschmidt, M., Rachfall, N., Schulze, F., López Marín, S., Hoppert, M., Streckfuss-Bömeke, K., Fischer, C., Braus, G.H. Mol. Cell Proteomics (2007) [Pubmed]
  9. The transcriptional regulator Hap1p (Cyp1p) is essential for anaerobic or heme-deficient growth of Saccharomyces cerevisiae: Genetic and molecular characterization of an extragenic suppressor that encodes a WD repeat protein. Chantrel, Y., Gaisne, M., Lions, C., Verdière, J. Genetics (1998) [Pubmed]
  10. One-step affinity purification of the yeast ribosome and its associated proteins and mRNAs. Inada, T., Winstall, E., Tarun, S.Z., Yates, J.R., Schieltz, D., Sachs, A.B. RNA (2002) [Pubmed]
 
WikiGenes - Universities