Disease relevance of SRV2

• C. albicans strains with deletions of both alleles of CAP1 were avirulent in a mouse model of systemic candidiasis [1].

High impact information on SRV2

• These results demonstrate that SRV2 protein is required for RAS-activated adenylate cyclase activity, but that it participates in other essential cellular functions as well [2].
• We have identified a gene, SRV2, mutations of which alleviate stress sensitivity in strains carrying an activated RAS gene [2].
• A high-affinity interaction with ADP-actin monomers underlies the mechanism and in vivo function of Srv2/cyclase-associated protein [3].
• Abp1p contains a single SH3 domain that has recently been shown to bind in vitro to the adenylyl cyclase-associated protein Srv2p [4].
• In addition we have isolated a recessive negative allele of SRV2 that is defective for endocytosis [5].

Biological context of SRV2

• In the yeast, Saccharomyces cerevisiae, adenylyl cyclase consists of a 200-kDa catalytic subunit (CYR1) and a 70-kDa subunit (CAP/SRV2) [6].
• This protein shares significant sequence homology with the adenylyl cyclase-associated CAP protein in the yeast Saccharomyces cerevisiae [7].
• The adenylyl-cyclase-associated protein, CAP, was originally identified in yeasts as a protein that functions in both signal transduction and cytoskeletal organization [8].
• Adenylyl cyclase-associated protein Aca1 regulates virulence and differentiation of Cryptococcus neoformans via the cyclic AMP-protein kinase A cascade [9].

Anatomical context of SRV2

• Evidence for physical and functional interactions among two Saccharomyces cerevisiae SH3 domain proteins, an adenylyl cyclase-associated protein and the actin cytoskeleton [4].
• Using three steps of screening, including differential hybridization, sequence analyses and in situ hybridization, a clone encoding a rat homolog of yeast adenylyl cyclase-associated protein (CAP) was identified to be highly expressed in a subset of taste bud cells [10].

Associations of SRV2 with chemical compounds

• Mutants with decreased kinase activity resulting from a srv2 mutation showed no alterations in cisplatin sensitivity [11].
• Association of elongation factor 1 alpha and ribosomal protein L3 with the proline-rich region of yeast adenylyl cyclase-associated protein CAP [12].

Physical interactions of SRV2

• In search for novel actin binding proteins in Dictyostelium discoideum we have isolated a cDNA clone coding for a protein of approximately 50 kDa that is highly homologous to the class of adenylyl cyclase-associated proteins (CAP) [13].

Other interactions of SRV2

• Furthermore, this domain has an endocytic function that is redundant with the function(s) of ABP1 and SRV2 [5].
• However, we found three classes of these proteins: WASP, Srv2/CAP and verprolin/WIP in yeast and animals [14].

References