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Gene Review

CAP1  -  CAP, adenylate cyclase-associated protein...

Homo sapiens

Synonyms: Adenylyl cyclase-associated protein 1, CAP, CAP 1, CAP1-PEN
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Disease relevance of CAP1

  • Treatment of male rats with ornidazole results in reversible infertility, which is associated with the detection of the contraception-associated protein 1 (CAP1) in epididymal fluid [1].
  • These results indicate that the inhibition ELISA with MAb CAP1 is effective in detection of circulating SAP antigen and that this assay may be useful for diagnosis and treatment monitoring of invasive candidiasis [2].
  • We have examined the susceptibility of adenylyl cyclase-associated Gi and 'Gp'-activated phospholipase C to inhibitory ADP-ribosylation by pertussis toxin-derived enzyme (S1 protomer) administered to saponin-permeabilized platelets [3].

High impact information on CAP1


Biological context of CAP1

  • SDS-PAGE analysis revealed that the migration pattern for hamster CAP1 compared to the other rodent counterparts, rat and mouse was different; indicating species-specific differences in the protein (possibly due to post-translational modifications) [7].
  • Results of the present studies suggest that MoAb CAP1 recognizes a receptor-induced binding site on canine fibrinogen [8].
  • A monoclonal antibody to human platelet glycoprotein IIIa (GPIIIa), which cross-reacts with canine platelet GPIIIa regardless of platelet activation status, did not inhibit binding of MoAb CAP1 [8].
  • Using mouse expressed sequence tag sequences that are highly homologous to the published human prostasin sequence as templates, reverse transcription-polymerase chain reaction and RACE (rapid amplification of cDNA ends) were used to sequence mouse prostasin mRNA, which shows 99% identical to published mouse CAP1 sequence [9].
  • CAP-1 had a Rebers-Riddiford consensus sequence, which is conserved in cuticle proteins from many arthropods [10].

Anatomical context of CAP1

  • It was noticed that hamster CAP1 was lost from the mid piece of spermatozoa during capacitation [7].
  • Flow cytometric analysis of sperm from ornidazole-fed rats revealed a significant decline (of 22%-24%) in the amount of sperm surface CAP1 compared with controls, which is associated with an altered location on the sperm head [1].
  • Immunolocalization of CAP1 in rat testis showed a strong staining of elongating spermatids (stages VI-VIII), indicating a translational delay of CAP1 expression [1].
  • Detection of RNA expression in rats at the cellular level by in situ hybridization revealed a stage-specific CAP1 expression in the cytoplasm of pachytene spermatocytes (stages IX-XIII), secondary spermatocytes (stage XIV), and round spermatids (stages I-VII) [1].
  • Binding of MoAb CAP1 could be partially restored by activating gel-filtered platelets with PAF, suggesting that the antigen was either present within platelet granule membranes or was exposed after binding of released proteins(s) with a platelet receptor [8].

Associations of CAP1 with chemical compounds

  • MoAb CAP1 binding was diminished by ethylenediamine-tetraacetic acid, suggesting that the antigen was either stabilized by calcium or antigen binding to the platelet surface was mediated by calcium [8].
  • MoAb CAP1 bound to isolated canine fibrinogen captured on polystyrene microtiter plates in the absence of platelet proteins [8].
  • ADP-activated gel-filtered platelets also demonstrated reduced binding of MoAB CAP1 even in the presence of 1 mM CaCl2 [8].
  • An apical serine protease, channel-activating protease 1 (CAP1), augments sodium transport in A6 cells [9].
  • Two different schedules of cisplatin were used: 20 mg/m2 day 1-->5 (CAP 5), or 80 mg/m2 on day 1 (CAP 1) [11].

Other interactions of CAP1

  • On the other hand, amino terminal sequences of the isoenzymes were found to be 79% and 87% homologous with CAP1 and CPA2 of the rat, respectively [12].
  • A monoclonal antibody (MAb; MAb CAP1) that was reactive with extracellular aspartic proteinase of Candida albicans (CAP) was produced [13].
  • Prostasin, a novel serine protease originally purified from seminal fluid, has been proposed to be the mammalian ortholog of CAP1 [9].

Analytical, diagnostic and therapeutic context of CAP1


  1. Expression and cellular localization of contraception-associated protein. Wagenfeld, A., Yeung, C.H., Shivaji, S., Sundareswaran, V.R., Ariga, H., Cooper, T.G. J. Androl. (2000) [Pubmed]
  2. Use of monoclonal antibody in diagnosis of candidiasis caused by Candida albicans: detection of circulating aspartyl proteinase antigen. Na, B.K., Song, C.Y. Clin. Diagn. Lab. Immunol. (1999) [Pubmed]
  3. Regulation of platelet phospholipase C. Rittenhouse, S.E., Banga, H.S., Sasson, J.P., King, W.G., Tarver, A.P. Philos. Trans. R. Soc. Lond., B, Biol. Sci. (1988) [Pubmed]
  4. Antisera against a guanine nucleotide binding protein from retina cross-react with the beta subunit of the adenylyl cyclase-associated guanine nucleotide binding proteins, Ns and Ni. Gierschik, P., Codina, J., Simons, C., Birnbaumer, L., Spiegel, A. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  5. Identification of an enhancer agonist cytotoxic T lymphocyte peptide from human carcinoembryonic antigen. Zaremba, S., Barzaga, E., Zhu, M., Soares, N., Tsang, K.Y., Schlom, J. Cancer Res. (1997) [Pubmed]
  6. Different expression patterns of calpain isozymes 1 and 2 (CAPN1 and 2) in squamous cell carcinomas (SCC) and basal cell carcinomas (BCC) of human skin. Reichrath, J., Welter, C., Mitschele, T., Classen, U., Meineke, V., Tilgen, W., Seifert, M. J. Pathol. (2003) [Pubmed]
  7. Hamster contraception associated protein 1 (CAP1). Siva, A.B., Sundareswaran, V.R., Yeung, C.H., Cooper, T.G., Shivaji, S. Mol. Reprod. Dev. (2004) [Pubmed]
  8. A platelet activation-specific monoclonal antibody that recognizes a receptor-induced binding site on canine fibrinogen. Boudreaux, M.K., Panangala, V.S., Bourne, C. Vet. Pathol. (1996) [Pubmed]
  9. Serine protease activity in m-1 cortical collecting duct cells. Liu, L., Hering-Smith, K.S., Schiro, F.R., Hamm, L.L. Hypertension (2002) [Pubmed]
  10. Purification and structural determination of a phosphorylated peptide with anti-calcification and chitin-binding activities in the exoskeleton of the crayfish, Procambarus clarkii. Inoue, H., Ozaki, N., Nagasawa, H. Biosci. Biotechnol. Biochem. (2001) [Pubmed]
  11. Results of the combination of cisplatin, adriamycin and cyclophosphamide in the treatment of ovarian carcinoma. Russo, A., Gebbia, V., Palmeri, S., Geraci, P., Maneschi, F., Guarnieri, G., Carollo, F., Leonardi, V., Meli, M., Gebbia, N. Eur. J. Gynaecol. Oncol. (1993) [Pubmed]
  12. Separation of human pancreatic carboxypeptidase A isoenzymes by high performance liquid chromatography. Linder, D., Linder, M., Schade, H., Sziegoleit, A. Biomed. Chromatogr. (1993) [Pubmed]
  13. Production, characterization, and epitope mapping of a monoclonal antibody against aspartic proteinase of Candida albicans. Na, B.K., Chung, G.T., Song, C.Y. Clin. Diagn. Lab. Immunol. (1999) [Pubmed]
  14. Specific cytolytic T-cell responses to human CEA from patients immunized with recombinant avipox-CEA vaccine. Zhu, M.Z., Marshall, J., Cole, D., Schlom, J., Tsang, K.Y. Clin. Cancer Res. (2000) [Pubmed]
  15. Chemotherapy consisting of cisplatin, epirubicin, and cyclophosphamide in advanced ovarian carcinoma. The Gynecologic Oncology Group of the Comprehensive Cancer Center, Limburg, The Netherlands. Stoot, J., Wils, J., von Geuns, H., Smeets, J., Schouten, L. Cancer Invest. (1993) [Pubmed]
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