The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

RNR1  -  ribonucleotide-diphosphate reductase...

Saccharomyces cerevisiae S288c

Synonyms: CRT7, RIR1, Ribonucleoside-diphosphate reductase large chain 1, Ribonucleotide reductase R1 subunit 1, Ribonucleotide reductase large subunit 1, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on RNR1

  • In the presence of DNA damage this association is disrupted and Rnr2/Rnr4 become cytoplasmic, where they join with Rnr1 to form an intact complex [1].
  • RNR1 is inducible 3- to 5-fold, and RNR3 is inducible greater than 100-fold [2].
  • Analysis of mRNA levels in cell-cycle-synchronized cultures reveals that the RNR1 mRNA is tightly cell-cycle regulated, fluctuating 15- to 30-fold, and is coordinately regulated with the POL1 mRNA, being expressed in the late G1 and S phases of the cell cycle [2].
  • Progression from the alpha-factor-induced G1 block to induction of RNR1 mRNA is blocked by cycloheximide, further defining the requirement for protein synthesis in the G1- to S-phase transition [2].
  • In addition, sml1 affects various cellular processes analogous to overproducing the large subunit of ribonucleotide reductase, RNR1 [3].

Biological context of RNR1

  • It is encoded by three genes, RNR1, RNR2 and RNR3, each of which is inducible by agents that damage DNA or block DNA replication [4].
  • Class I RNRs are composed of two types of subunits: RNR1 contains the active site for reduction and the binding sites for the nucleotide allosteric effectors [5].
  • The cell cycle-dependent transcription of the RNR1 and CLN1 genes is also described and the implications for cell cycle control, in G1, are discussed with reference to these two genes [6].
  • We find that during meiotic development a subset of DNA replication genes exemplified by TMP1 and RNR1 are regulated by Mbp1 [7].
  • In addition, we found that over-expression of RNR1, encoding a large subunit of ribonucleotide reductase (RNR), rescued the petite-induction phenotype of a pif1Delta mutation to a similar extent as deletion of RRM3 [8].

Associations of RNR1 with chemical compounds


Physical interactions of RNR1


Regulatory relationships of RNR1

  • Yeast DNA damage-inducible Rnr3 has a very low catalytic activity strongly stimulated after the formation of a cross-talking Rnr1/Rnr3 complex [10].

Other interactions of RNR1

  • The in vitro activity of Rnr3 was less than 1% of the Rnr1 activity [10].
  • An impact on ribonucleotide reduction was seen by an increased accumulation of RNR1 and RNR2 transcripts in the thioredoxin mutant (4.3- and 6.8-fold, respectively) [11].
  • In some crt groups, the expression of RNR1 and RNR2 are also elevated, suggesting that all three RNR genes share a common regulatory pathway [4].
  • In a cln1 cln2 background, a prolonged period of expression of genes turned on at the G1-S border, such as RNR1, has been observed [12].
  • Northern analysis demonstrated that RNR1 expression is reduced by CLN1 or CLN2 overexpression [12].


  1. Control of ribonucleotide reductase localization through an anchoring mechanism involving Wtm1. Lee, Y.D., Elledge, S.J. Genes Dev. (2006) [Pubmed]
  2. Two genes differentially regulated in the cell cycle and by DNA-damaging agents encode alternative regulatory subunits of ribonucleotide reductase. Elledge, S.J., Davis, R.W. Genes Dev. (1990) [Pubmed]
  3. A suppressor of two essential checkpoint genes identifies a novel protein that negatively affects dNTP pools. Zhao, X., Muller, E.G., Rothstein, R. Mol. Cell (1998) [Pubmed]
  4. Isolation of crt mutants constitutive for transcription of the DNA damage inducible gene RNR3 in Saccharomyces cerevisiae. Zhou, Z., Elledge, S.J. Genetics (1992) [Pubmed]
  5. Purification of ribonucleotide reductase subunits Y1, Y2, Y3, and Y4 from yeast: Y4 plays a key role in diiron cluster assembly. Nguyen, H.H., Ge, J., Perlstein, D.L., Stubbe, J. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  6. A general approach to the isolation of cell cycle-regulated genes in the budding yeast, Saccharomyces cerevisiae. Price, C., Nasmyth, K., Schuster, T. J. Mol. Biol. (1991) [Pubmed]
  7. Meiosis-specific regulation of the Saccharomyces cerevisiae S-phase cyclin CLB5 is dependent on MluI cell cycle box (MCB) elements in its promoter but is independent of MCB-binding factor activity. Raithatha, S.A., Stuart, D.T. Genetics (2005) [Pubmed]
  8. Differential involvement of the related DNA helicases Pif1p and Rrm3p in mtDNA point mutagenesis and stability. O'Rourke, T.W., Doudican, N.A., Zhang, H., Eaton, J.S., Doetsch, P.W., Shadel, G.S. Gene (2005) [Pubmed]
  9. Photochemical surface mapping of C14S-Sml1p for constrained computational modeling of protein structure. Sharp, J.S., Guo, J.T., Uchiki, T., Xu, Y., Dealwis, C., Hettich, R.L. Anal. Biochem. (2005) [Pubmed]
  10. Yeast DNA damage-inducible Rnr3 has a very low catalytic activity strongly stimulated after the formation of a cross-talking Rnr1/Rnr3 complex. Domkin, V., Thelander, L., Chabes, A. J. Biol. Chem. (2002) [Pubmed]
  11. Deoxyribonucleotides are maintained at normal levels in a yeast thioredoxin mutant defective in DNA synthesis. Muller, E.G. J. Biol. Chem. (1994) [Pubmed]
  12. Interaction between the MEC1-dependent DNA synthesis checkpoint and G1 cyclin function in Saccharomyces cerevisiae. Vallen, E.A., Cross, F.R. Genetics (1999) [Pubmed]
WikiGenes - Universities