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RNR1  -  ribonucleotide-diphosphate reductase...

Saccharomyces cerevisiae S288c

Synonyms: CRT7, RIR1, Ribonucleoside-diphosphate reductase large chain 1, Ribonucleotide reductase R1 subunit 1, Ribonucleotide reductase large subunit 1, ...
 
 
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High impact information on RNR1

  • In the presence of DNA damage this association is disrupted and Rnr2/Rnr4 become cytoplasmic, where they join with Rnr1 to form an intact complex [1].
  • RNR1 is inducible 3- to 5-fold, and RNR3 is inducible greater than 100-fold [2].
  • Analysis of mRNA levels in cell-cycle-synchronized cultures reveals that the RNR1 mRNA is tightly cell-cycle regulated, fluctuating 15- to 30-fold, and is coordinately regulated with the POL1 mRNA, being expressed in the late G1 and S phases of the cell cycle [2].
  • Progression from the alpha-factor-induced G1 block to induction of RNR1 mRNA is blocked by cycloheximide, further defining the requirement for protein synthesis in the G1- to S-phase transition [2].
  • In addition, sml1 affects various cellular processes analogous to overproducing the large subunit of ribonucleotide reductase, RNR1 [3].
 

Biological context of RNR1

  • It is encoded by three genes, RNR1, RNR2 and RNR3, each of which is inducible by agents that damage DNA or block DNA replication [4].
  • Class I RNRs are composed of two types of subunits: RNR1 contains the active site for reduction and the binding sites for the nucleotide allosteric effectors [5].
  • The cell cycle-dependent transcription of the RNR1 and CLN1 genes is also described and the implications for cell cycle control, in G1, are discussed with reference to these two genes [6].
  • We find that during meiotic development a subset of DNA replication genes exemplified by TMP1 and RNR1 are regulated by Mbp1 [7].
  • In addition, we found that over-expression of RNR1, encoding a large subunit of ribonucleotide reductase (RNR), rescued the petite-induction phenotype of a pif1Delta mutation to a similar extent as deletion of RRM3 [8].
 

Associations of RNR1 with chemical compounds

 

Physical interactions of RNR1

 

Regulatory relationships of RNR1

  • Yeast DNA damage-inducible Rnr3 has a very low catalytic activity strongly stimulated after the formation of a cross-talking Rnr1/Rnr3 complex [10].
 

Other interactions of RNR1

  • The in vitro activity of Rnr3 was less than 1% of the Rnr1 activity [10].
  • An impact on ribonucleotide reduction was seen by an increased accumulation of RNR1 and RNR2 transcripts in the thioredoxin mutant (4.3- and 6.8-fold, respectively) [11].
  • In some crt groups, the expression of RNR1 and RNR2 are also elevated, suggesting that all three RNR genes share a common regulatory pathway [4].
  • In a cln1 cln2 background, a prolonged period of expression of genes turned on at the G1-S border, such as RNR1, has been observed [12].
  • Northern analysis demonstrated that RNR1 expression is reduced by CLN1 or CLN2 overexpression [12].

References

  1. Control of ribonucleotide reductase localization through an anchoring mechanism involving Wtm1. Lee, Y.D., Elledge, S.J. Genes Dev. (2006) [Pubmed]
  2. Two genes differentially regulated in the cell cycle and by DNA-damaging agents encode alternative regulatory subunits of ribonucleotide reductase. Elledge, S.J., Davis, R.W. Genes Dev. (1990) [Pubmed]
  3. A suppressor of two essential checkpoint genes identifies a novel protein that negatively affects dNTP pools. Zhao, X., Muller, E.G., Rothstein, R. Mol. Cell (1998) [Pubmed]
  4. Isolation of crt mutants constitutive for transcription of the DNA damage inducible gene RNR3 in Saccharomyces cerevisiae. Zhou, Z., Elledge, S.J. Genetics (1992) [Pubmed]
  5. Purification of ribonucleotide reductase subunits Y1, Y2, Y3, and Y4 from yeast: Y4 plays a key role in diiron cluster assembly. Nguyen, H.H., Ge, J., Perlstein, D.L., Stubbe, J. Proc. Natl. Acad. Sci. U.S.A. (1999) [Pubmed]
  6. A general approach to the isolation of cell cycle-regulated genes in the budding yeast, Saccharomyces cerevisiae. Price, C., Nasmyth, K., Schuster, T. J. Mol. Biol. (1991) [Pubmed]
  7. Meiosis-specific regulation of the Saccharomyces cerevisiae S-phase cyclin CLB5 is dependent on MluI cell cycle box (MCB) elements in its promoter but is independent of MCB-binding factor activity. Raithatha, S.A., Stuart, D.T. Genetics (2005) [Pubmed]
  8. Differential involvement of the related DNA helicases Pif1p and Rrm3p in mtDNA point mutagenesis and stability. O'Rourke, T.W., Doudican, N.A., Zhang, H., Eaton, J.S., Doetsch, P.W., Shadel, G.S. Gene (2005) [Pubmed]
  9. Photochemical surface mapping of C14S-Sml1p for constrained computational modeling of protein structure. Sharp, J.S., Guo, J.T., Uchiki, T., Xu, Y., Dealwis, C., Hettich, R.L. Anal. Biochem. (2005) [Pubmed]
  10. Yeast DNA damage-inducible Rnr3 has a very low catalytic activity strongly stimulated after the formation of a cross-talking Rnr1/Rnr3 complex. Domkin, V., Thelander, L., Chabes, A. J. Biol. Chem. (2002) [Pubmed]
  11. Deoxyribonucleotides are maintained at normal levels in a yeast thioredoxin mutant defective in DNA synthesis. Muller, E.G. J. Biol. Chem. (1994) [Pubmed]
  12. Interaction between the MEC1-dependent DNA synthesis checkpoint and G1 cyclin function in Saccharomyces cerevisiae. Vallen, E.A., Cross, F.R. Genetics (1999) [Pubmed]
 
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