The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

phoB  -  transcriptional regulator PhoB

Escherichia coli O157:H7 str. EDL933

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of phoB


High impact information on phoB

  • PhoB protein binding sites on the promoter regions of pstS and phoB were determined by footprinting experiments with DNase I and a methylating agent [6].
  • This plasmid enabled us to study phoB gene expression by measuring the beta-galactosidase level in the cells [7].
  • A mutation in phoB was constructed in the S. flexneri pst mutant, and the phoB mutation suppressed the small plaque phenotype of the pst mutant [8].
  • While phoB was not required for stalk synthesis or for the cell cycle timing of stalk synthesis initiation, it was required for stalk elongation in response to phosphate starvation [9].
  • Introduction of a phoB mutation in the wild-type strain eliminated elevated levels of acetate kinase and phosphotransacetylase activities in response to phosphate limitation and also eliminated the phosphate stress-induced up-regulation of the ackA::lacZ fusion [1].

Biological context of phoB


Associations of phoB with chemical compounds

  • Insertion of a kanamycin resistance cassette into phoB led to impaired growth of the B. japonicum mutant in media containing approximately 25 microM phosphate or less [4].
  • Three classes of TC45-resistant strains were found: (i) mutants in phoB, a regulatory gene for protein e, (ii) mutants with an altered lipopolysaccharide, and (iii) mutants unaltered in the regulation of the pho regulon and producing an apparently normal lipopolysaccharide [11].
  • A phoB-cat fusion that expressed chloramphenicol transacetylase from the phoB promoter was constructed [12].

Other interactions of phoB

  • The transcriptome profiles of the wild-type and the phoB mutant strains were compared at the time point showing the highest expression levels of the phoB and phoR genes under a P-limiting condition [13].


  1. Genes coding for phosphotransacetylase and acetate kinase in Sinorhizobium meliloti are in an operon that is inducible by phosphate stress and controlled by phoB. Summers, M.L., Denton, M.C., McDermott, T.R. J. Bacteriol. (1999) [Pubmed]
  2. Inorganic polyphosphate in Escherichia coli: the phosphate regulon and the stringent response. Rao, N.N., Liu, S., Kornberg, A. J. Bacteriol. (1998) [Pubmed]
  3. Use of a phoB'-'lacZ fusion gene to determine the N-terminal amino acid sequence of the phoB protein and to prepare antiserum against the protein. Sugita, T., Shinagawa, H., Makino, K., Nakata, A. J. Biochem. (1985) [Pubmed]
  4. The Bradyrhizobium japonicum phoB gene is required for phosphate-limited growth but not for symbiotic nitrogen fixation. Minder, A.C., Narberhaus, F., Fischer, H.M., Hennecke, H. FEMS Microbiol. Lett. (1998) [Pubmed]
  5. Nucleotide sequence of the Pseudomonas aeruginosa phoB gene, the regulatory gene for the phosphate regulon. Anba, J., Bidaud, M., Vasil, M.L., Lazdunski, A. J. Bacteriol. (1990) [Pubmed]
  6. Regulation of the phosphate regulon of Escherichia coli. Activation of pstS transcription by PhoB protein in vitro. Makino, K., Shinagawa, H., Amemura, M., Kimura, S., Nakata, A., Ishihama, A. J. Mol. Biol. (1988) [Pubmed]
  7. Regulation of the pho regulon in Escherichia coli K-12. Genetic and physiological regulation of the positive regulatory gene phoB. Shinagawa, H., Makino, K., Nakata, A. J. Mol. Biol. (1983) [Pubmed]
  8. Role of the Pst system in plaque formation by the intracellular pathogen Shigella flexneri. Runyen-Janecky, L.J., Boyle, A.M., Kizzee, A., Liefer, L., Payne, S.M. Infect. Immun. (2005) [Pubmed]
  9. Regulation of stalk elongation by phosphate in Caulobacter crescentus. Gonin, M., Quardokus, E.M., O'Donnol, D., Maddock, J., Brun, Y.V. J. Bacteriol. (2000) [Pubmed]
  10. Control of the synthesis of alkaline phosphatase and the phosphate-binding protein in Escherichia coli. Willsky, G.R., Malamy, M.H. J. Bacteriol. (1976) [Pubmed]
  11. Localization of phoE, the structural gene for outer membrane protein e in Escherichia coli K-12. Tommassen, J., Lugtenberg, B. J. Bacteriol. (1981) [Pubmed]
  12. Analysis of regulation of phoB expression using a phoB-cat fusion. Guan, C.D., Wanner, B., Inouye, H. J. Bacteriol. (1983) [Pubmed]
  13. Novel gene members in the Pho regulon of Escherichia coli. Baek, J.H., Lee, S.Y. FEMS Microbiol. Lett. (2006) [Pubmed]
WikiGenes - Universities