The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

trpB  -  tryptophan synthase, beta subunit

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1255, JW1253
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of trpB


High impact information on trpB


Chemical compound and disease context of trpB

  • The trpB and trpA coding regions of the polycistronic trp mRNA of Escherichia coli are separated by overlapping translation stop and start codons [8].

Biological context of trpB

  • Most of the nucleotide sequences can be assigned to specific locations in the structural genes, based on the amino-acid sequences of the trpB and trpA proteins [7].
  • The resulting phylogeny was congruent with trees derived from sequences of two chromosome-located bacterial genes (part of trpB and 16S ribosomal DNA) [9].
  • This modification avoids the polar effects encountered when either an untranslated sequence, or a sequence translated in a different reading frame is fused to trpB [10].
  • The expression of the trpB gene was remarkably enhanced (>12-fold) by the introduction of an overlapping leader open reading frame [11].
  • Transformants were obtained with circular forms of the SAG1/trpB plasmid with efficiencies of 10(-4) per cell [5].

Associations of trpB with chemical compounds

  • The trpA and trpB genes encoding the tryptophan synthase subunits were cloned from the hyperthermophilic archaeon Pyrococcus furiosus [11].
  • The trp poL was used successfully to control the expression of lac alpha, luxAB, trpB and trpA [12].

Other interactions of trpB

  • In N. gonorrhoeae, the genes trpB and ksgA were found immediately downstream of iga [3].

Analytical, diagnostic and therapeutic context of trpB


  1. Nucleotide sequence of the trpB gene in Escherichia coli and Salmonella typhimurium. Crawford, I.P., Nichols, B.P., Yanofsky, C. J. Mol. Biol. (1980) [Pubmed]
  2. Ordering tryptophan synthase genes of Pseudomonas aeruginosa by cloning in Escherichia coli. Manch, J.N., Crawford, I.P. J. Bacteriol. (1981) [Pubmed]
  3. The integration site of the iga gene in commensal Neisseria sp. Jose, J., Otto, G.W., Meyer, T.F. Mol. Genet. Genomics (2003) [Pubmed]
  4. Synechocystis PCC 6803 contains a single gene for the beta subunit of tryptophan synthase with strong homology to the trpB genes of Arabidopsis and maize (Zea mays L.). Zhao, G.P., Somerville, R.L., Chitnis, P.R. Plant Physiol. (1994) [Pubmed]
  5. Stable DNA transformation in the obligate intracellular parasite Toxoplasma gondii by complementation of tryptophan auxotrophy. Sibley, L.D., Messina, M., Niesman, I.R. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  6. Two dominant-acting selectable markers for gene transfer studies in mammalian cells. Hartman, S.C., Mulligan, R.C. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  7. An intercistronic region and ribosome-binding site in bacterial messenger RNA. Platt, T., Yanofsky, C. Proc. Natl. Acad. Sci. U.S.A. (1975) [Pubmed]
  8. Restoration of a translational stop-start overlap reinstates translational coupling in a mutant trpB'-trpA gene pair of the Escherichia coli tryptophan operon. Das, A., Yanofsky, C. Nucleic Acids Res. (1989) [Pubmed]
  9. The tryptophan biosynthetic pathway of aphid endosymbionts (Buchnera): genetics and evolution of plasmid-associated anthranilate synthase (trpEG) within the aphididae. Rouhbakhsh, D., Lai, C.Y., von Dohlen, C.D., Clark, M.A., Baumann, L., Baumann, P., Moran, N.A., Voegtlin, D.J. J. Mol. Evol. (1996) [Pubmed]
  10. A versatile multiple- and single-copy vector system for the in vitro construction of transcriptional fusions to lacZ. Linn, T., Ralling, G. Plasmid (1985) [Pubmed]
  11. Overexpression in Escherichia coli of the AT-rich trpA and trpB genes from the hyperthermophilic archaeon Pyrococcus furiosus. Ishida, M., Oshima, T., Yutani, K. FEMS Microbiol. Lett. (2002) [Pubmed]
  12. PCR based gene engineering of the Vibrio harveyi lux operon and the Escherichia coli trp operon provides for biochemically functional native and fused gene products. Hill, P.J., Swift, S., Stewart, G.S. Mol. Gen. Genet. (1991) [Pubmed]
WikiGenes - Universities