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Gene Review

rcsB  -  response regulator in two-component...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK2210, JW2205, viaA
 
 
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Disease relevance of rcsB

  • Isolation and characterization of rcsB mutations that affect colanic acid capsule synthesis in Escherichia coli K-12 [1].
  • Characterization of the rcsB gene from Erwinia amylovora and its influence on exoploysaccharide synthesis and virulence of the fire blight pathogen [2].
 

High impact information on rcsB

 

Biological context of rcsB

  • Here, we report the cloning and DNA sequence of a 4.3-kb region containing rcsC and rcsB from E. coli O9:K30:H12 [5].
  • The rcsB and rcsC genes, which are oriented convergently with their stop codons 196 base pairs apart, are separated by a long direct repeat including two repetitive extragenic palindromic sequences [6].
  • Analysis of the 5' upstream sequence of rcsB revealed, in addition to the sigma 54 promoter sequence previously reported, a presumptive sigma 70 promoter and LexA-binding site plus an upstream sequence that is found to be essential for the expression of rcsB on a plasmid [7].
  • Regulation of osmC gene expression by the two-component system rcsB-rcsC in Escherichia coli [8].
 

Other interactions of rcsB

  • Characterization of rcsB and rcsC from Escherichia coli O9:K30:H12 and examination of the role of the rcs regulatory system in expression of group I capsular polysaccharides [5].
  • The rcsB gene, a positive regulator of colanic acid biosynthesis in Escherichia coli, is also an activator of ftsZ expression [7].
 

Analytical, diagnostic and therapeutic context of rcsB

  • The rcsB gene of the fire blight pathogen Erwinia amylovora was cloned by PCR amplification with consensus primers, and its role in exopolysaccharide (EPS) synthesis was investigated [2].
  • Inactivation of rcsB by site-directed mutagenesis created mutants that were deficient in amylovoran synthesis and avirulent on host plants [2].
  • All the spontaneous viaA mutants isolated from E. coli and S. typhi were identified as rcsB mutants by complementation tests using plasmid pJB100 [9].

References

  1. Isolation and characterization of rcsB mutations that affect colanic acid capsule synthesis in Escherichia coli K-12. Gupte, G., Woodward, C., Stout, V. J. Bacteriol. (1997) [Pubmed]
  2. Characterization of the rcsB gene from Erwinia amylovora and its influence on exoploysaccharide synthesis and virulence of the fire blight pathogen. Bereswill, S., Geider, K. J. Bacteriol. (1997) [Pubmed]
  3. Acetyl phosphate-sensitive regulation of flagellar biogenesis and capsular biosynthesis depends on the Rcs phosphorelay. Fredericks, C.E., Shibata, S., Aizawa, S., Reimann, S.A., Wolfe, A.J. Mol. Microbiol. (2006) [Pubmed]
  4. Dual regulatory pathways integrating the RcsC-RcsD-RcsB signalling system control enterohaemorrhagic Escherichia coli pathogenicity. Tobe, T., Ando, H., Ishikawa, H., Abe, H., Tashiro, K., Hayashi, T., Kuhara, S., Sugimoto, N. Mol. Microbiol. (2005) [Pubmed]
  5. Characterization of rcsB and rcsC from Escherichia coli O9:K30:H12 and examination of the role of the rcs regulatory system in expression of group I capsular polysaccharides. Jayaratne, P., Keenleyside, W.J., MacLachlan, P.R., Dodgson, C., Whitfield, C. J. Bacteriol. (1993) [Pubmed]
  6. RcsB and RcsC: a two-component regulator of capsule synthesis in Escherichia coli. Stout, V., Gottesman, S. J. Bacteriol. (1990) [Pubmed]
  7. The rcsB gene, a positive regulator of colanic acid biosynthesis in Escherichia coli, is also an activator of ftsZ expression. Gervais, F.G., Phoenix, P., Drapeau, G.R. J. Bacteriol. (1992) [Pubmed]
  8. Regulation of osmC gene expression by the two-component system rcsB-rcsC in Escherichia coli. Davalos-Garcia, M., Conter, A., Toesca, I., Gutierrez, C., Cam, K. J. Bacteriol. (2001) [Pubmed]
  9. Expression of Vi antigen in Escherichia coli K-12: characterization of ViaB from Citrobacter freundii and identity of ViaA with RcsB. Houng, H.S., Noon, K.F., Ou, J.T., Baron, L.S. J. Bacteriol. (1992) [Pubmed]
 
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