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Gene Review

rph  -  pseudo

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3633, JW3618, orfE
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Disease relevance of rph


High impact information on rph


Chemical compound and disease context of rph


Biological context of rph

  • DNA sequencing revealed that a GC base pair was missing near the end of the rph gene of W3110 [1].
  • The cloned B. subtilis rph gene also suppresses the cold-sensitive phenotype of other unrelated cold-sensitive mutants of E. coli, but not the temperature-sensitive phenotype of three temperature-sensitive mutants, including the nusA11(Ts) mutant, that were tested [9].
  • Attenuation in the rph-pyrE operon of Escherichia coli and processing of the dicistronic mRNA [10].
  • The responsible gene encodes an open reading frame that is greater than 50% identical at the amino acid level to the E. coli rph gene, which was formerly called orfE [9].
  • Inactivation of these genes (designated orfE, orfX and orfY) by crossing the KmR-cassette-disrupted orf into the chromosome indicated that they were not required during exponential growth (Poulsen et al., Mol., Microbiol., 1989 b) [11].

Anatomical context of rph

  • Expression of the Escherichia coli pyrE gene is regulated by transcription attenuation in the intercistronic orfE-pyrE region and modulated by the distance between the transcribing RNA polymerase and the leading ribosome as a function of the supply of UTP and GTP [12].


  1. The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels. Jensen, K.F. J. Bacteriol. (1993) [Pubmed]
  2. Amplification of the Bacillus subtilis maf gene results in arrested septum formation. Butler, Y.X., Abhayawardhane, Y., Stewart, G.C. J. Bacteriol. (1993) [Pubmed]
  3. Identification of a new inhibitor of essential division gene ftsZ as the kil gene of defective prophage Rac. Conter, A., Bouché, J.P., Dassain, M. J. Bacteriol. (1996) [Pubmed]
  4. Conserved domains in polynucleotide phosphorylase among eubacteria. Bermúdez-Cruz, R.M., Ramírez, F., Kameyama-Kawabe, L., Montañez, C. Biochimie (2005) [Pubmed]
  5. Identification of the gene encoding the Escherichia coli lipid A 4'-kinase. Facile phosphorylation of endotoxin analogs with recombinant LpxK. Garrett, T.A., Kadrmas, J.L., Raetz, C.R. J. Biol. Chem. (1997) [Pubmed]
  6. Overexpression and rapid purification of the orfE/rph gene product, RNase PH of Escherichia coli. Jensen, K.F., Andersen, J.T., Poulsen, P. J. Biol. Chem. (1992) [Pubmed]
  7. The essential Escherichia coli msbA gene, a multicopy suppressor of null mutations in the htrB gene, is related to the universally conserved family of ATP-dependent translocators. Karow, M., Georgopoulos, C. Mol. Microbiol. (1993) [Pubmed]
  8. Molecular and mutational analysis of three genes preceding pyrE on the Escherichia coli chromosome. Poulsen, P., Andersen, J.T., Jensen, K.F. Mol. Microbiol. (1989) [Pubmed]
  9. Identification of the rph (RNase PH) gene of Bacillus subtilis: evidence for suppression of cold-sensitive mutations in Escherichia coli. Craven, M.G., Henner, D.J., Alessi, D., Schauer, A.T., Ost, K.A., Deutscher, M.P., Friedman, D.I. J. Bacteriol. (1992) [Pubmed]
  10. Attenuation in the rph-pyrE operon of Escherichia coli and processing of the dicistronic mRNA. Andersen, J.T., Poulsen, P., Jensen, K.F. Eur. J. Biochem. (1992) [Pubmed]
  11. Three genes preceding pyrE on the Escherichia coli chromosome are essential for survival and normal cell morphology in stationary culture and at high temperature. Poulsen, P., Jensen, K.F. Res. Microbiol. (1991) [Pubmed]
  12. Role of transcription pausing in the control of the pyrE attenuator in Escherichia coli. Andersen, J.T., Jensen, K.F., Poulsen, P. Mol. Microbiol. (1991) [Pubmed]
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