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Klc  -  Kinesin light chain

Drosophila melanogaster

Synonyms: CG5433, DKLC, Dmel\CG5433, KLC, kinesin, ...
 
 
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Disease relevance of Klc

  • KLP68D protein produced in Escherichia coli is, like kinesin itself, a plus-end directed microtubule motor [1].
  • Antibodies purified from these sera by their affinity for brain kinesin react with a polypeptide of approximately 120 kD in extracts from bovine brain, PtK1 cells, and mouse neuroblastoma cells [2].
  • When BDTC (a 1.3-S subunit of Propionibacterium shermanii transcarboxylase, which binds weakly to a microtubule), was fused to the tail (C-terminus) of K351, its movement was enhanced, smooth, and unidirectional, similar to that of the two-headed kinesin fragment, K411 [3].
 

Psychiatry related information on Klc

  • Loss of Klc function results in progressive lethargy, crawling defects, and paralysis followed by death at the end of the second larval instar [4].
  • The microtubule motor activity of kinesin is performed by the heavy chains, but the functions of the light chains are poorly understood [4].
 

High impact information on Klc

  • Mutations in sunday driver (syd) and the axonal transport motor kinesin-I cause similar phenotypes in Drosophila, including aberrant accumulations of axonal cargoes [5].
  • We propose that SYD mediates the axonal transport of at least one class of vesicles by interacting directly with KLC [5].
  • Members of the kinesin superfamily share a similar motor catalytic domain yet move either toward the plus end (e.g., conventional kinesin) or the minus end (e.g., Ncd) of microtubules [6].
  • The directional preference of kinesin motors is specified by an element outside of the motor catalytic domain [6].
  • Hedgehog elicits signal transduction by means of a large complex containing the kinesin-related protein costal2 [7].
 

Biological context of Klc

 

Anatomical context of Klc

  • Klc mutant axons contain large aggregates of membranous organelles in segmental nerve axons [4].
  • We find that milton, a gene whose product was previously shown to associate with Kinesin and to mediate axonal transport of mitochondria, is needed to form a normal Balbiani body [11].
  • BACKGROUND: Motor proteins of the minus end-directed cytoplasmic dynein and plus end-directed kinesin families provide the principal means for microtubule-based transport in eukaryotic cells [12].
  • Kinesin mutations cause motor neuron disease phenotypes by disrupting fast axonal transport in Drosophila [13].
  • An epitope-tagged YETI fusion protein, when expressed in Drosophila S2 cultured cells, binds to kinesin-I in copurification assays, suggesting that YETI-kinesin-I interactions are context-independent [14].
 

Associations of Klc with chemical compounds

 

Physical interactions of Klc

 

Regulatory relationships of Klc

  • When not bound to cargo, the motor protein kinesin is in an inhibited state that has low microtubule-stimulated ATPase activity [20].
 

Other interactions of Klc

 

Analytical, diagnostic and therapeutic context of Klc

References

  1. Characterization of the KLP68D kinesin-like protein in Drosophila: possible roles in axonal transport. Pesavento, P.A., Stewart, R.J., Goldstein, L.S. J. Cell Biol. (1994) [Pubmed]
  2. Localization of kinesin in cultured cells. Neighbors, B.W., Williams, R.C., McIntosh, J.R. J. Cell Biol. (1988) [Pubmed]
  3. Motility of single one-headed kinesin molecules along microtubules. Inoue, Y., Iwane, A.H., Miyai, T., Muto, E., Yanagida, T. Biophys. J. (2001) [Pubmed]
  4. Kinesin light chains are essential for axonal transport in Drosophila. Gindhart, J.G., Desai, C.J., Beushausen, S., Zinn, K., Goldstein, L.S. J. Cell Biol. (1998) [Pubmed]
  5. Kinesin-dependent axonal transport is mediated by the sunday driver (SYD) protein. Bowman, A.B., Kamal, A., Ritchings, B.W., Philp, A.V., McGrail, M., Gindhart, J.G., Goldstein, L.S. Cell (2000) [Pubmed]
  6. The directional preference of kinesin motors is specified by an element outside of the motor catalytic domain. Case, R.B., Pierce, D.W., Hom-Booher, N., Hart, C.L., Vale, R.D. Cell (1997) [Pubmed]
  7. Hedgehog elicits signal transduction by means of a large complex containing the kinesin-related protein costal2. Robbins, D.J., Nybakken, K.E., Kobayashi, R., Sisson, J.C., Bishop, J.M., Thérond, P.P. Cell (1997) [Pubmed]
  8. Disruption of axonal transport and neuronal viability by amyloid precursor protein mutations in Drosophila. Gunawardena, S., Goldstein, L.S. Neuron (2001) [Pubmed]
  9. Kinesin light chain-independent function of the Kinesin heavy chain in cytoplasmic streaming and posterior localisation in the Drosophila oocyte. Palacios, I.M., St Johnston, D. Development (2002) [Pubmed]
  10. The Drosophila kinesin light chain. Primary structure and interaction with kinesin heavy chain. Gauger, A.K., Goldstein, L.S. J. Biol. Chem. (1993) [Pubmed]
  11. Milton controls the early acquisition of mitochondria by Drosophila oocytes. Cox, R.T., Spradling, A.C. Development (2006) [Pubmed]
  12. The cytoplasmic dynein and kinesin motors have interdependent roles in patterning the Drosophila oocyte. Duncan, J.E., Warrior, R. Curr. Biol. (2002) [Pubmed]
  13. Kinesin mutations cause motor neuron disease phenotypes by disrupting fast axonal transport in Drosophila. Hurd, D.D., Saxton, W.M. Genetics (1996) [Pubmed]
  14. The Drosophila kinesin-I associated protein YETI binds both kinesin subunits. Wisniewski, T.P., Tanzi, C.L., Gindhart, J.G. Biol. Cell (2003) [Pubmed]
  15. Microtubule-kinesin interface mutants reveal a site critical for communication. Klumpp, L.M., Brendza, K.M., Gatial, J.E., Hoenger, A., Saxton, W.M., Gilbert, S.P. Biochemistry (2004) [Pubmed]
  16. Minus-end-directed motion of kinesin-coated microspheres driven by microtubule depolymerization. Lombillo, V.A., Stewart, R.J., McIntosh, J.R. Nature (1995) [Pubmed]
  17. Nucleotide-dependent angular change in kinesin motor domain bound to tubulin. Hirose, K., Lockhart, A., Cross, R.A., Amos, L.A. Nature (1995) [Pubmed]
  18. Chromophore-assisted light inactivation and self-organization of microtubules and motors. Surrey, T., Elowitz, M.B., Wolf, P.E., Yang, F., Nédélec, F., Shokat, K., Leibler, S. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  19. Mechanical and chemical properties of cysteine-modified kinesin molecules. Iwatani, S., Iwane, A.H., Higuchi, H., Ishii, Y., Yanagida, T. Biochemistry (1999) [Pubmed]
  20. Kinesin's tail domain is an inhibitory regulator of the motor domain. Coy, D.L., Hancock, W.O., Wagenbach, M., Howard, J. Nat. Cell Biol. (1999) [Pubmed]
  21. Kinesin-II is required for axonal transport of choline acetyltransferase in Drosophila. Ray, K., Perez, S.E., Yang, Z., Xu, J., Ritchings, B.W., Steller, H., Goldstein, L.S. J. Cell Biol. (1999) [Pubmed]
  22. An N-terminal truncation of the ncd motor protein supports diffusional movement of microtubules in motility assays. Chandra, R., Endow, S.A., Salmon, E.D. J. Cell. Sci. (1993) [Pubmed]
  23. Expression, purification, and characterization of the Drosophila kinesin motor domain produced in Escherichia coli. Gilbert, S.P., Johnson, K.A. Biochemistry (1993) [Pubmed]
  24. Enhancement of the ncdD microtubule motor mutant by mutants of alpha Tub67C. Komma, D.J., Endow, S.A. J. Cell. Sci. (1997) [Pubmed]
  25. Crystal structure of the motor domain of the kinesin-related motor ncd. Sablin, E.P., Kull, F.J., Cooke, R., Vale, R.D., Fletterick, R.J. Nature (1996) [Pubmed]
  26. Purification of novel kinesins from embryonic systems. Meyer, D., Rines, D.R., Kashina, A., Cole, D.G., Scholey, J.M. Meth. Enzymol. (1998) [Pubmed]
  27. Structures of kinesin and kinesin-microtubule interactions. Mandelkow, E., Hoenger, A. Curr. Opin. Cell Biol. (1999) [Pubmed]
 
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