High impact information on Peptide Biosynthesis

• Accordingly, we have investigated the effect of nicotinic receptor stimulation on Met-enkephalin peptide biosynthesis and expression of proenkephalin messenger RNA in primary cultures of bovine chromaffin cells [1].
• As axons and terminals have very little capacity for peptide biosynthesis, it is possible that substance P is synthesised and packaged in the perikaryon and transported to the terminals by an axoplasmic transport process [2].
• While there is some evidence that CPE may be regulated, it does not appear that regulation of CPE activity plays an important role in controlling peptide biosynthesis [3].
• Taken together, these results support a major role for PC2 as the pro-NPY converting enzyme, and they establish the cultured SCG neurons as a model to study neuronal peptide biosynthesis [4].
• The stimulatory effect on peptide biosynthesis appears to be specific for cAMP, as bag cell cGMP levels were not altered significantly by high potassium or forskolin, and 8-bromo-cGMP did not stimulate ELH synthesis [5].

Biological context of Peptide Biosynthesis

• These results indicate that glucocorticoid treatment is required for maximal proenkephalin gene expression and EC peptide biosynthesis in cultured glands [6].
• Regulation of cyclic peptide biosynthesis and pathogenicity in Cochliobolus carbonum by TOXEp, a novel protein with a bZIP basic DNA-binding motif and four ankyrin repeats [7].

Associations of Peptide Biosynthesis with chemical compounds

• Despite these similarities, conserved cysteine residues found in the latter synthetases and thought to be essential for the thiotemplate mechanism of peptide biosynthesis have not been detected in the ACVS sequence [8].
• The low efficiency of D-glucose as an insulin secretagogue contrasts with a marked stimulant action of the hexose on peptide biosynthesis [9].
• A role for the adenylate cyclase pathway in regulating GLI peptide biosynthesis is also suggested [10].
• Labeling with d10-Leu was also used to test whether altering the pH of the secretory pathway with chloroquine affected the rate of peptide biosynthesis [11].
• Two potential sites for drug targeting were identified: fatty acid synthesis, for all strains assayed, and peptide biosynthesis, particularly for Rg colony variants that possess previously identified phenylalanine-containing lipopeptides as potential targets for future drug development [12].

Gene context of Peptide Biosynthesis

• The results suggest that excitatory amino acid transmission mediated by the NMDA receptor serves as a tonic signal to stimulate neuroactive peptide biosynthesis [13].
• A eukaryotic alanine racemase gene involved in cyclic peptide biosynthesis [14].
• PAM catalyzes the final step in alpha-amidated peptide biosynthesis, and its proposed role in primary fatty acid amide biosynthesis has been controversial [15].
• The unique biosynthetic and enzymic patterns seen for PACE4 suggest a role for this neuroendocrine-specific subtilisin-like endoprotease outside the pathway for peptide biosynthesis [16].
• These results suggest that the decreases in hypothalamic POMC peptide levels after E administration reported previously may be due to a decrease in POMC peptide biosynthesis resulting from a decrease in hypothalamic POMC mRNA [17].

Analytical, diagnostic and therapeutic context of Peptide Biosynthesis

• Site-specific mutagenesis has been performed in thiolation, elongation, and epimerization motifs of some of the modules of surfactin synthetase from B. subtilis to clarify the function of prominent conserved amino acid residues in the intermediate steps of peptide biosynthesis [18].

References