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Chordoma: Other Cell Lines

GB60 and CH8
  • MTT assay found that cells from the CH8 and GB60 chordoma cell lines proliferated more rapidly in IMDM and DMEM media, but less rapidly in RPMI 1640. [1]
  • The novel CH8 chordoma cell line contained cells with chordoma-like morphology and gene transcripts that clustered close to chordoma tissue and cell lines upon gene array analysis. [1]
  • A cell line (EACH-1) was established from a human extra-axial chordoma after culturing the primary tumor, transplanting the cultured cells into the posterior abdominal flank of an immunocompromised nude mouse, removing the newly grown tumor 12 weeks post-transplantation, and growing the secondary tumor cells into a cell line. The doubling time for EACH-1 cultures was observed to be 37 hours. [2]
  • Western blot analysis showed that cells from the EACH-1 cell line exhibited decreased brachyury expression with the number of passages. After 30 passages, the cell line entered into crisis. [2]
  • FACS analysis on cells from the EACH-1 cell line found 91% positive for CD49f, 95% positive for CD49b, 92% positive for CD29, 96% positive for CD44, 93% positive for CD49c, 95% positive for CD59, 96% positive for CD73a, 97% positive for CD105, 94% positive for CD166, 5% positive for CD90, 27% positive for CD147, 2% positive for CD36, 3% positive for CD34, 1% positive for CD11b, 3% positive for CD19, 3% positive for CD45, 5% positive for CD117, 20% positive for CD3, 5% positive for CD184, 7% positive for CD79a, 7% positive for CD106, and 4% positive for CD133. [2]
  • Cytogenetic analysis by the G-bands method using trypsin and Wright staining on 21 metaphases for the EACH-1 cell line revealed unrelated diploid or hypotetraploid clones with aberrations. These aberrations included chromosomal rearrangements and loss (1p and 2p) or gain (5q and 20) of a part or the whole of multiple chromosomes. [2]
  • EACH-1 cells were observed to form colonies after 3 weeks of growth under non-adherent conditions, and this ability to form colonies was retained when the cells were incubated in suspension during 6 or 12 days. [2]
  • Serial subcutaneous transplantation of EACH-1 cells into nude mice resulted in tumor formation after 3-4 weeks. These tumor cells resembled the primary tumor cells that were used to establish the EACH-1 cell line. [2]
  • RT-PCR analysis revealed expression of MRP1 and HIF-1α in the CM-319 chordoma cell line–expression of MDR1 was not detected. [3]
  • Western blot analysis revealed expression of MDR1, MRP1, and HIF-1α in the CM-319 chordoma cell line. [3]
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  1. Characterization and analysis of human chordoma cell lines. Yang, C., Hornicek, F.J., Wood, K.B., Schwab, J.H., Choy, E., Iafrate, J., Rosenberg, A., Nielsen, G.P., Xavier, R.J., Mankin, H., Duan, Z. Spine. (Phila. Pa. 1976). (2010) [Pubmed]
  2. Derivation and characterization of an extra-axial chordoma cell line (EACH-1) from a scapular tumor. DeComas, A.M., Penfornis, P., Harris, M.R., Meyer, M.S., Pochampally, R.R. J. Bone. Joint. Surg. Am. (2010) [Pubmed]
  3. Expression of MDR1, HIF-1α and MRP1 in sacral chordoma and chordoma cell line CM-319. Ji, Z., Long, H., Hu, Y., Qiu, X., Chen, X., Li, Z., Fan, D., Ma, B., Fan, Q. J. Exp. Clin. Cancer. Res. (2010) [Pubmed]
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