The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Chemical Compound Review

Carboxybiotin     (1S,2S,5S)-2-(4- carboxybutyl)-7-oxo-3-thia...

Synonyms: AG-J-18165, KST-1A8195, AC1Q5VZP, CTK2F6624, AR-1A4043, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of Carboxybiotin


High impact information on Carboxybiotin


Biological context of Carboxybiotin

  • The membrane-bound beta-subunit is responsible for the decarboxylation of carboxybiotin and the coupled translocation of Na+ ions across the membrane [7].
  • The results are consistent with the hypothesis that the location of the carboxybiotin in the active site is determined by the presence of Mg2+, acetyl-CoA and the oxo acid substrate [8].
  • The kinetics of the decay of the enzyme-[14C] carboxybiotin complex at 0 degree C in the absence of substrates are similar to the reaction with pyruvate except that the carboxybiotin is also unstable in the first sub-site, to some degree [9].
  • Biotin assay excluded potential contamination by traces of biotin as a cause of the observed ATP hydrolysis, and this was confirmed by the findings that carboxybiotin did not accumulate and that avidin was uninhibitory [10].
  • In many sequenced genomes from Bacteria and Archaea homologues of the carboxybiotin decarboxylase from A. fermentans with up to 80% sequence identity have been detected [11].

Associations of Carboxybiotin with other chemical compounds

  • The model predicts that asp203 in its dissociated form binds Na+ and promotes its translocation, while the protonated residue transfers the proton to the acid-labile carboxybiotin which initiates its decarboxylation [7].
  • Recently it has been shown that the proton required for the decarboxylation of carboxybiotin is taken up from the side to which Na+ ions are pumped, and a membrane-embedded aspartate residue that is probably involved both in Na+ and in H+ transport was identified [12].

Gene context of Carboxybiotin

  • The carboxybiotin complex of pyruvate carboxylase. A kinetic analysis of the effects of Mg2+ ions on its stability and on its reaction with pyruvate [9].
  • MadB is the integral membrane-bound carboxybiotin protein decarboxylase, MadC and MadD are the two subunits of the carboxyltransferase, MadE is the acyl carrier protein and MadF is the biotin protein [13].


  1. Essential role of tyrosine 229 of the oxaloacetate decarboxylase beta-subunit in the energy coupling mechanism of the Na(+) pump. Jockel, P., Schmid, M., Choinowski, T., Dimroth, P. Biochemistry (2000) [Pubmed]
  2. Crystal structure of the carboxyltransferase subunit of the bacterial sodium ion pump glutaconyl-coenzyme A decarboxylase. Wendt, K.S., Schall, I., Huber, R., Buckel, W., Jacob, U. EMBO J. (2003) [Pubmed]
  3. Molecular structure and intermolecular interactions of N1'-methoxycarbonylbiotin methyl ester: a model for carboxybiotin. Stallings, W.C., Monti, C.T., Lane, M.D., DeTitta, G.T. Proc. Natl. Acad. Sci. U.S.A. (1980) [Pubmed]
  4. A molecular coupling mechanism for the oxaloacetate decarboxylase Na+ pump as inferred from mutational analysis. Jockel, P., Schmid, M., Steuber, J., Dimroth, P. Biochemistry (2000) [Pubmed]
  5. Role of CO2 in proton activation by histidine decarboxylase (pyruvoyl). Rose, I.A., Kuo, D.J. Biochemistry (1992) [Pubmed]
  6. The carboxybiotin complex of chicken liver pyruvate carboxylase. A kinetic analysis of the effects of acetyl-CoA, Mg2+ ions and temperature on its stability and on its reaction with 2-oxobutyrate. Attwood, P.V., Wallace, J.C. Biochem. J. (1986) [Pubmed]
  7. Aspartate 203 of the oxaloacetate decarboxylase beta-subunit catalyses both the chemical and vectorial reaction of the Na+ pump. Di Berardino, M., Dimroth, P. EMBO J. (1996) [Pubmed]
  8. Factors that influence the translocation of the N-carboxybiotin moiety between the two sub-sites of pyruvate carboxylase. Goodall, G.J., Baldwin, G.S., Wallace, J.C., Keech, D.B. Biochem. J. (1981) [Pubmed]
  9. The carboxybiotin complex of pyruvate carboxylase. A kinetic analysis of the effects of Mg2+ ions on its stability and on its reaction with pyruvate. Attwood, P.V., Wallace, J.C., Keech, D.B. Biochem. J. (1984) [Pubmed]
  10. ATPase activity of biotin carboxylase provides evidence for initial activation of HCO3- by ATP in the carboxylation of biotin. Climent, I., Rubio, V. Arch. Biochem. Biophys. (1986) [Pubmed]
  11. Sodium ion-translocating decarboxylases. Buckel, W. Biochim. Biophys. Acta (2001) [Pubmed]
  12. Anaerobic citrate metabolism and its regulation in enterobacteria. Bott, M. Arch. Microbiol. (1997) [Pubmed]
  13. Sequence of a gene cluster from Malonomonas rubra encoding components of the malonate decarboxylase Na+ pump and evidence for their function. Berg, M., Hilbi, H., Dimroth, P. Eur. J. Biochem. (1997) [Pubmed]
WikiGenes - Universities