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Chemical Compound Review

Ketobutyrate     2-oxobutanoic acid

Synonyms: a-Oxobutyrate, a-Ketobutyrate, K401_ALDRICH, CHEMBL171246, ACMC-1AW4C, ...
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Disease relevance of oxobutyrate


High impact information on oxobutyrate


Chemical compound and disease context of oxobutyrate


Biological context of oxobutyrate


Anatomical context of oxobutyrate


Associations of oxobutyrate with other chemical compounds

  • Previous studies have proposed that some HI0719 homologues may act on small molecules in the isoleucine biosynthetic path and, if this is correct, the ligand screening results presented here suggest that the interaction most likely occurs with 2-ketobutyrate and/or its unstable enamine precursor [21].
  • We overcome this problem by preparing a protein using a 98% D2O cell culture medium containing 3-(13)C pyruvic acid, 50-60% deuterated at the 3-position, and 4-(13)C 2-ketobutyric acid, 98% and 62% deuterated at the 3- and 4-positions, respectively [22].
  • Inhibitory antibodies to BCODH and an inhibitor of PDH (3-fluoropyruvate) were used with mitochondrial extracts to determine the relative contribution of both complexes to oxidative decarboxylation of 2-oxobutyrate [16].
  • Importantly, much smaller effects were observed for the kinetic parameters of reactions with the alternate substrates L-vinylglycine (L-VG) (deamination to form alpha-ketobutyrate and ammonia) and L-alanine (transamination to form pyruvate), which have uncharged side chains [23].
  • Alternatively, in the threonine dehydratase pathway, the 1-carbon ends up in alpha-ketobutyrate, which is oxidized in the mitochondria to CO(2) [24].

Gene context of oxobutyrate


Analytical, diagnostic and therapeutic context of oxobutyrate


  1. Crystal Structures of Salmonella typhimurium Biodegradative Threonine Deaminase and Its Complex with CMP Provide Structural Insights into Ligand-induced Oligomerization and Enzyme Activation. Simanshu, D.K., Savithri, H.S., Murthy, M.R. J. Biol. Chem. (2006) [Pubmed]
  2. Cloning, expression, purification, and characterization of biosynthetic threonine deaminase from Escherichia coli. Eisenstein, E. J. Biol. Chem. (1991) [Pubmed]
  3. Threonine dehydratases of Corynebacterium glutamicum with altered allosteric control: their generation and biochemical and structural analysis. Möckel, B., Eggeling, L., Sahm, H. Mol. Microbiol. (1994) [Pubmed]
  4. Structural analysis of Pseudomonas 1-aminocyclopropane-1-carboxylate deaminase complexes: insight into the mechanism of a unique pyridoxal-5'-phosphate dependent cyclopropane ring-opening reaction. Karthikeyan, S., Zhou, Q., Zhao, Z., Kao, C.L., Tao, Z., Robinson, H., Liu, H.W., Zhang, H. Biochemistry (2004) [Pubmed]
  5. On the mechanism of the glucose-induced ATP catabolism in ascites tumour cells and its reversal by pyruvate. Glaser, G., Giloh, H., Kasir, J., Gross, M., Mager, J. Biochem. J. (1980) [Pubmed]
  6. Metabolic activation and detoxication of nephrotoxic cysteine and homocysteine S-conjugates. Elfarra, A.A., Lash, L.H., Anders, M.W. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
  7. Energetics of cooperative ligand binding to the active sites of biosynthetic threonine deaminase from Escherichia coli. Eisenstein, E. J. Biol. Chem. (1994) [Pubmed]
  8. Enzymatic reactions of methionine sulfoximine. Conversion to the corresponding alpha-imino and alpha-keto acids and to alpha-ketobutyrate and methane sulfinimide. Cooper, A.J., Stephani, R.A., Meister, A. J. Biol. Chem. (1976) [Pubmed]
  9. Novel keto acid formate-lyase and propionate kinase enzymes are components of an anaerobic pathway in Escherichia coli that degrades L-threonine to propionate. Hesslinger, C., Fairhurst, S.A., Sawers, G. Mol. Microbiol. (1998) [Pubmed]
  10. Recognition of structurally diverse substrates by type II 3-hydroxyacyl-CoA dehydrogenase (HADH II)/amyloid-beta binding alcohol dehydrogenase (ABAD). Powell, A.J., Read, J.A., Banfield, M.J., Gunn-Moore, F., Yan, S.D., Lustbader, J., Stern, A.R., Stern, D.M., Brady, R.L. J. Mol. Biol. (2000) [Pubmed]
  11. Conversion of Escherichia coli pyruvate oxidase to an 'alpha-ketobutyrate oxidase'. Chang, Y.Y., Cronan, J.E. Biochem. J. (2000) [Pubmed]
  12. Reaction mechanism of phosphoenolpyruvate carboxylase. Bicarbonate-dependent dephosphorylation of phosphoenol-alpha-ketobutyrate. Fujita, N., Izui, K., Nishino, T., Katsuki, H. Biochemistry (1984) [Pubmed]
  13. Toxic accumulation of alpha-ketobutyrate caused by inhibition of the branched-chain amino acid biosynthetic enzyme acetolactate synthase in Salmonella typhimurium. LaRossa, R.A., Van Dyk, T.K., Smulski, D.R. J. Bacteriol. (1987) [Pubmed]
  14. Mutations in two distinct regions of acetolactate synthase regulatory subunit from Streptomyces cinnamonensis result in the lack of sensitivity to end-product inhibition. Kopecký, J., Janata, J., Pospísil, S., Felsberg, J., Spízek, J. Biochem. Biophys. Res. Commun. (1999) [Pubmed]
  15. Conversion of DL-threonine, D-threonine and 2-oxobutyrate into propionate and 2-hydroxybutyrate by Fusobacterium species. Carlier, J.P., Henry, C., Lorin, V., Rouffignat, K. Lett. Appl. Microbiol. (1997) [Pubmed]
  16. Role of branched-chain 2-oxo acid dehydrogenase and pyruvate dehydrogenase in 2-oxobutyrate metabolism. Paxton, R., Scislowski, P.W., Davis, E.J., Harris, R.A. Biochem. J. (1986) [Pubmed]
  17. Characterization of alpha-ketobutyrate metabolism in rat tissues: effects of dietary protein and fasting. Steele, R.D., Weber, H., Patterson, J.I. J. Nutr. (1984) [Pubmed]
  18. 2-Hydroxyacid dehydrogenase from Haloferax mediterranei, a D-isomer-specific member of the 2-hydroxyacid dehydrogenase family. Bonete, M.J., Ferrer, J., Pire, C., Penades, M., Ruiz, J.L. Biochimie (2000) [Pubmed]
  19. Normal 2-aminobutyrate oxidation and increased valine oxidation in fibroblasts deficient in pyruvate dehydrogenase. Borud, O., Pettersen, J.E. J. Inherit. Metab. Dis. (1982) [Pubmed]
  20. Effect of alpha-ketobutyrate on palmitic acid and pyruvate metabolism in isolated rat hepatocytes. Brass, E.P. Biochim. Biophys. Acta (1986) [Pubmed]
  21. Solution structure and functional ligand screening of HI0719, a highly conserved protein from bacteria to humans in the YjgF/YER057c/UK114 family. Parsons, L., Bonander, N., Eisenstein, E., Gilson, M., Kairys, V., Orban, J. Biochemistry (2003) [Pubmed]
  22. Optimized labeling of 13CHD2 methyl isotopomers in perdeuterated proteins: potential advantages for 13C relaxation studies of methyl dynamics of larger proteins. Ishima, R., Louis, J.M., Torchia, D.A. J. Biomol. NMR (2001) [Pubmed]
  23. Glutamate 47 in 1-aminocyclopropane-1-carboxylate synthase is a major specificity determinant. McCarthy, D.L., Capitani, G., Feng, L., Gruetter, M.G., Kirsch, J.F. Biochemistry (2001) [Pubmed]
  24. Threonine metabolism in isolated rat hepatocytes. House, J.D., Hall, B.N., Brosnan, J.T. Am. J. Physiol. Endocrinol. Metab. (2001) [Pubmed]
  25. Synthesis of the isoleucyl- and valyl-tRNA synthetases and the isoleucine-valine biosynthetic enzymes in a threonine deaminase regulatory mutant of Escherichia coli K-12. Singer, P.A., Levinthal, M., Williams, L.S. J. Mol. Biol. (1984) [Pubmed]
  26. Crystal structure of the pyridoxal-5'-phosphate-dependent serine dehydratase from human liver. Sun, L., Bartlam, M., Liu, Y., Pang, H., Rao, Z. Protein Sci. (2005) [Pubmed]
  27. The purification and properties of urocanase from Pseudomonas testosteroni. Hacking, A.J., Bell, M.V., Hassall, H. Biochem. J. (1978) [Pubmed]
  28. Anaplerotic reactions in tumour proliferation and apoptosis. Quash, G., Fournet, G., Reichert, U. Biochem. Pharmacol. (2003) [Pubmed]
  29. Metabolic effects of inhibitors of two enzymes of the branched-chain amino acid pathway in Salmonella typhimurium. Epelbaum, S., Chipman, D.M., Barak, Z. J. Bacteriol. (1996) [Pubmed]
  30. High performance liquid chromatography determination of cystathionase activity in human and rabbit lenses. Bergad, P.L., Rathbun, W.B. Ophthalmic Res. (1986) [Pubmed]
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