Disease relevance of Oxanosine

• The mechanism of antitumor action of oxanosine was studied using a strain of rat kidney cells infected with a mutant Rous sarcoma virus, the src gene of which was temperature sensitive [1].
• Thus the differential toxicity of oxanosine and 5-azacytidine at the two temperatures is thought to be due to their increased transport via the thymidine-transport system, which is somehow under the influence of the active src-gene product [2].
• Oxanosine 3r, 5-amino-3-beta-(d-ribofuranosyl)-3H-imidazo[4,5-d][1,3]oxazine-7-one, was isolated as a novel nucleoside antibiotic in 1981 from Streptomyces capreolus MG265-CF3 [3].
• Synergistic anti-viral effect of oxanosine and ddI against human immunodeficiency virus [4].
• Oxanosine, a novel nucleoside from actinomycetes [5].

High impact information on Oxanosine

• Oxanosine also increased the cellular content of fibronectin to the normal level [6].
• These changes in p21 were the same as those which occurred to p21 in the cells cultured in the absence of oxanosine at 39.5 degrees C, a nonpermissive temperature [6].
• Increased sensitivity to oxanosine, a novel nucleoside antibiotic, of rat kidney cells upon expression of the integrated viral src gene [1].
• Oxanosine inhibited cell growth in vitro, as well as nucleic acid synthesis in these cells, 10 times more strongly at a permissive temperature (33 degrees C) than at a non-permissive temperature (39 degrees C) [1].
• The reaction products xanthosine 3r and oxanosine 4r were separated by HPLC and characterized by LC/MS analysis and 13C NMR spectroscopy [7].

Chemical compound and disease context of Oxanosine

• The growth of leukemia L 1210 cell was also inhibited by oxanosine, and the inhibition was reversed by guanylic acid [8].

Biological context of Oxanosine

• Oxanosine is a substrate of adenosine deaminase. Implications for the quest for a toxicological marker for nitrosation activity [3].
• The hydrolysis of 3 to 4 can be rationalized on the basis of the known mode of action of ADA, and an explanation is provided for ADA's accomplishment of the "usual" substitution at C6 of adenosine (addition to the exocyclic bond) and the "lactone hydrolysis" of oxanosine (addition to the endocyclic double bond) [3].
• All four oxanosine-containing base pairs (A/O, T/O, C/O and G/O) were cleaved with similar efficiency [9].
• Oxanosine inhibited the growth of HeLa cells in vitro (IC50 32 mcg/ml) and suppressed the growth of L-1210 leukemia in mice [10].

Gene context of Oxanosine

• Unexpectedly, it was discovered that the incubation of oxanosine 3r with ADA in sodium phosphate buffer (pH = 7.4) results in 1-beta-(d-ribofuranosyl)-5-ureido-1H-imidazole-4-carboxylic acid 4r [3].
• Oxanosine was confirmed to be a competitive inhibitor of GMP synthetase (E.C. 6.3.5.2) and the Ki value was 7.4 X 10(-4) M [8].
• The primary action of oxanosine appears to be inhibition of GMP-synthetase [10].

Analytical, diagnostic and therapeutic context of Oxanosine

• Intravenous injection of 4 mg of oxanosine to mice does not show any toxic sign [10].

References