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Gene Review

rpsL  -  30S ribosomal protein S12

Escherichia coli CFT073

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Disease relevance of rpsL

  • The plasmid contains the rpsL gene of Escherichia coli as a mutational target gene, and the kanamycin-resistance gene for recovering the plasmid from the chromosomal DNA [1].
  • Thermus rpsL mutations were found to be very similar to rpsL mutations identified in mesophilic organisms [2].
  • The ribosomal protein (r-protein)-encoding gene, rpsL, and regions flanking it, from Salmonella typhimurium, have been sequenced directly from polymerase chain reaction-amplified chromosomal DNA [3].

High impact information on rpsL

  • Here, we show that the level of transcription of the rpsL gene strongly affects spontaneous mutagenesis at two mutational hotspot sites in the target sequence, one for a T-->G base substitution and the other for a+1 single-base frameshift [4].
  • All mutant phenotypes were found to result from single amino acid substitutions located in the rpsL gene encoding ribosomal protein S12 [2].
  • Physiological studies indicate that all three alleles are compatible with a number of different streptomycin resistance mutations (rpsL alleles) in a variety of genetic backgrounds [5].
  • Analysis of str mutants from Bacillus subtilis Marburg 168 revealed that a point mutation occurred within the rpsL gene, which encodes the ribosomal protein S12, changing Lys-56 (corresponding to Lys-43 in Escherichia coli) to Asn, Arg, Thr, or Gln [6].
  • These mutants affect translational accuracy and kinetics and suggest that Tet(O) and Tet(M) binding to the ribosome may be reduced or slowed in the E. coli rpsL mutants in which the S12 protein is altered [7].

Chemical compound and disease context of rpsL


Biological context of rpsL


Anatomical context of rpsL

  • Other classical rpsL mutations (K42N and K42T) tested did not show such an effect, indicating that this novel characteristic is typical of ribosomes bearing the K87E mutant form of S12, although the K87E mutation conferred the streptomycin resistance and error-restrictive phenotypes also seen with the K42N and K42T mutations [13].

Associations of rpsL with chemical compounds


  1. Transgenic zebrafish for detecting mutations caused by compounds in aquatic environments. Amanuma, K., Takeda, H., Amanuma, H., Aoki, Y. Nat. Biotechnol. (2000) [Pubmed]
  2. Streptomycin-resistant and streptomycin-dependent mutants of the extreme thermophile Thermus thermophilus. Gregory, S.T., Cate, J.H., Dahlberg, A.E. J. Mol. Biol. (2001) [Pubmed]
  3. The nucleotide sequence of rpsL and its flanking regions in Salmonella typhimurium. Hughes, D., Buckingham, R.H. Gene (1991) [Pubmed]
  4. Spontaneous hotspot mutations resistant to mismatch correction in Escherichia coli: transcription-dependent mutagenesis involving template-switching mechanisms. Yoshiyama, K., Maki, H. J. Mol. Biol. (2003) [Pubmed]
  5. Genetic characterization of early amber mutations in the Escherichia coli polA gene and purification of the amber peptides. Kelley, W.S., Joyce, C.M. J. Mol. Biol. (1983) [Pubmed]
  6. Acquisition of certain streptomycin-resistant (str) mutations enhances antibiotic production in bacteria. Hosoya, Y., Okamoto, S., Muramatsu, H., Ochi, K. Antimicrob. Agents Chemother. (1998) [Pubmed]
  7. Host mutations (miaA and rpsL) reduce tetracycline resistance mediated by Tet(O) and Tet(M). Taylor, D.E., Trieber, C.A., Trescher, G., Bekkering, M. Antimicrob. Agents Chemother. (1998) [Pubmed]
  8. Transcription and translation in a pleiotropic streptomycin-resistant mutant of Escherichia coli. Dennis, P.P. J. Bacteriol. (1979) [Pubmed]
  9. Rho and ribosome mutation interaction: lethality of rho-15 in rpsL or rpsE strains, and rho-15 methionine auxotrophy in rps+ strains of Escherichia coli. Guterman, S.K., Howitt, C.L. Genetics (1979) [Pubmed]
  10. DNA replication errors produced by the replicative apparatus of Escherichia coli. Fujii, S., Akiyama, M., Aoki, K., Sugaya, Y., Higuchi, K., Hiraoka, M., Miki, Y., Saitoh, N., Yoshiyama, K., Ihara, K., Seki, M., Ohtsubo, E., Maki, H. J. Mol. Biol. (1999) [Pubmed]
  11. Novel host-vector system for selection and maintenance of plasmid-bearing, streptomycin-dependent Escherichia coli cells in antibiotic-free media. Miwa, K., Nakamori, S., Sano, K., Momose, H. Gene (1984) [Pubmed]
  12. Exchange of chromosomal and plasmid alleles in Escherichia coli by selection for loss of a dominant antibiotic sensitivity marker. Russell, C.B., Dahlquist, F.W. J. Bacteriol. (1989) [Pubmed]
  13. The novel mutation K87E in ribosomal protein S12 enhances protein synthesis activity during the late growth phase in Escherichia coli. Hosaka, T., Tamehiro, N., Chumpolkulwong, N., Hori-Takemoto, C., Shirouzu, M., Yokoyama, S., Ochi, K. Mol. Genet. Genomics (2004) [Pubmed]
  14. Improvement of alpha-amylase production by modulation of ribosomal component protein S12 in Bacillus subtilis 168. Kurosawa, K., Hosaka, T., Tamehiro, N., Inaoka, T., Ochi, K. Appl. Environ. Microbiol. (2006) [Pubmed]
  15. A novel positive detection system of in vivo mutations in rpsL (strA) transgenic mice. Gondo, Y., Shioyama, Y., Nakao, K., Katsuki, M. Mutat. Res. (1996) [Pubmed]
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