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Gene Review

rpsE  -  30S ribosomal subunit protein S5

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3290, JW3265, eps, spc, spcA
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Disease relevance of rpsE

  • The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene [1].
  • We also constructed hybrid transducing phages that carry the IacZ gene fused to the promoter for the rrnE operon or to the promoter for the spc r protein operon [2].
  • The RNA polymerase binding sites on the DNA of (i) the aroE-trkA-spc segment of the Escherichia coli genome, (ii) transposon Tn3, (iii) plasmid ColE1, and (iv) coliphage lambda were mapped by electron microscopy, with the use of the BAC technique; these maps were compared with the maps of the early-melting regions for the same genomes [3].
  • Insofar as inactivation after Mu treatment is due to prophage insertion within or proximal to the genes in question, this result indicates that the genes strA and spcA are not parts of a single operon [4].
  • Cholera toxin secretion is dependent upon the extracellular protein secretion apparatus encoded by the eps gene locus of Vibrio cholerae [5].

High impact information on rpsE

  • A temperature-sensitive E. coli mutant with a mutation in the spc ribosomal protein operon was found to have a conditional defect in the processing of precursor proteins destined for the periplasmic space or the outer membrane [6].
  • The promoters from the str and spc operons of ribosomal proteins from E. coli were inserted into the Hind II cleavage site of mini-Col E1 (pVH51) plasmid [7].
  • DNA sequences of promoter regions for the str and spc ribosomal protein operons in E. coli [8].
  • Previous studies on regulation of the spc operon containing genes for ribosomal proteins have shown that S8, encoded by the fifth gene of the operon in Escherichia coli, is a translational repressor and regulates the synthesis of the third gene product (L5) and distal gene products by acting at a site near the L5 mRNA translation initiation site [9].
  • We suggest that repression of distal ribosomal protein synthesis by S8 triggers nucleolytic cleavage of spc operon mRNA, followed by mRNA degradation by these 3'- to 5'- exonucleases, which is then responsible for inhibition of L14-L24 synthesis [9].

Chemical compound and disease context of rpsE


Biological context of rpsE


Anatomical context of rpsE

  • Protein S8, a 129 amino acid component of the Escherichia coli ribosome, plays an essential role in the assembly of the 30S ribosomal subunit and in the translational regulation of the spc operon by virtue of its capacity to bind specifically to rRNA and mRNA [14].
  • Sequences of the following cyanelle genes encompassing 4.8 kb are reported here: 5'-rpl22-rps3-rpl16-rps17-rpl14-rpl5-rps8-rpl6-rpl18- rps5-3'. Cyanelles contain five more ribosomal protein genes than do higher plant chloroplasts and four more genes than Euglena gracilis plastids in the S10/spc region of this gene cluster [15].

Associations of rpsE with chemical compounds

  • The site of mutation is in the streptomycin (strA) region and is closely linked to the spcA gene [13].

Other interactions of rpsE


Analytical, diagnostic and therapeutic context of rpsE

  • The structure of the regulatory binding site, determined by sequence analysis of spc operon mRNA protected by S8 from RNase digestion, was found to contain all of the characteristic features of the 16 S rRNA binding site, demonstrating that the protein associates with structurally similar domains in both RNAs [17].


  1. The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene. Cerretti, D.P., Dean, D., Davis, G.R., Bedwell, D.M., Nomura, M. Nucleic Acids Res. (1983) [Pubmed]
  2. Growth-rate-dependent regulation of ribosome synthesis in E. coli: expression of the lacZ and galK genes fused to ribosomal promoters. Miura, A., Krueger, J.H., Itoh, S., de Boer, H.A., Nomura, M. Cell (1981) [Pubmed]
  3. A relationship between DNA helix stability and recognition sites for RNA polymerase. Vollenweider, H.J., Fiandt, M., Szybalski, W. Science (1979) [Pubmed]
  4. Expression of ribosomal protein genes in Escherichia coli. Cabezón, T., Faelen, M., De Wilde, M., Bollen, A., Thomas, R. Mol. Gen. Genet. (1975) [Pubmed]
  5. Identification of the Vibrio cholerae type 4 prepilin peptidase required for cholera toxin secretion and pilus formation. Marsh, J.W., Taylor, R.K. Mol. Microbiol. (1998) [Pubmed]
  6. A temperature-sensitive mutant of E. coli exhibiting slow processing of exported proteins. Ito, K., Wittekind, M., Nomura, M., Shiba, K., Yura, T., Miura, A., Nashimoto, H. Cell (1983) [Pubmed]
  7. Isolation and characterization of stable hybrid mRNA molecules transcribed from ribosomal protein promoters in E. coli. Ikemura, T., Itoh, S., Post, L.E., Nomura, M. Cell (1979) [Pubmed]
  8. DNA sequences of promoter regions for the str and spc ribosomal protein operons in E. coli. Post, L.E., Arfsten, A.E., Reusser, F., Nomura, M. Cell (1978) [Pubmed]
  9. Retroregulation of the synthesis of ribosomal proteins L14 and L24 by feedback repressor S8 in Escherichia coli. Mattheakis, L., Vu, L., Sor, F., Nomura, M. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  10. Insertion of IS1 into the rpsE gene for ribosomal protein S5 causes cold-sensitivity in Escherichia coli. Schnier, J., Isono, K. Mol. Gen. Genet. (1984) [Pubmed]
  11. Polarity of amber mutations in ribosomal protein genes of Escherichia coli. Cabezón, T., Delcuve, G., Faelen, M., Desmet, L., Bollen, A. J. Bacteriol. (1980) [Pubmed]
  12. Gene for the alpha subunit of Bacillus subtilis RNA polymerase maps in the ribosomal protein gene cluster. Suh, J.W., Boylan, S.A., Price, C.W. J. Bacteriol. (1986) [Pubmed]
  13. Suppression of spectinomycin resistance in a mutant of Escherichia coli K-12. Berg, P.E., Kang, S.S. J. Bacteriol. (1975) [Pubmed]
  14. Mutagenesis of ribosomal protein S8 from Escherichia coli: expression, stability, and RNA-binding properties of S8 mutants. Wu, H., Wower, I., Zimmermann, R.A. Biochemistry (1993) [Pubmed]
  15. The cyanelle S10 spc ribosomal protein gene operon from Cyanophora paradoxa. Michalowski, C.B., Pfanzagl, B., Löffelhardt, W., Bohnert, H.J. Mol. Gen. Genet. (1990) [Pubmed]
  16. Expression of lacZ from the promoter of the Escherichia coli spc operon cloned into vectors carrying the W205 trp-lac fusion. Liang, S.T., Dennis, P.P., Bremer, H. J. Bacteriol. (1998) [Pubmed]
  17. Interaction of Escherichia coli ribosomal protein S8 with its binding sites in ribosomal RNA and messenger RNA. Gregory, R.J., Cahill, P.B., Thurlow, D.L., Zimmermann, R.A. J. Mol. Biol. (1988) [Pubmed]
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