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Gene Review

rpsL  -  30S ribosomal subunit protein S12

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3329, JW3304, asuB, strA
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Disease relevance of rpsL

  • The rpsL gene of Escherichia coli encodes the highly conserved rps12 protein of the ribosomal accuracy centre [1].
  • Mutations were mapped by complementation using various transducing phages and plasmids carrying the rpsL gene, having either a normal or a defective promoter for the rpsL operon [2].
  • Spontaneous mutations in the Helicobacter pylori rpsL gene [3].
  • Although the methyltransferase mutant showed hypersensitivity to the alkylating reagent in terms of mutagenic effect and cell killing effects, the class and site distributions of the rpsL- mutations recovered from MNNG-treated ada- ogt- cells were similar to those observed with MNNG-treated wild-type cells [4].
  • Insofar as inactivation after Mu treatment is due to prophage insertion within or proximal to the genes in question, this result indicates that the genes strA and spcA are not parts of a single operon [5].

Psychiatry related information on rpsL


High impact information on rpsL

  • We find that the frequency of cysteine substitution for the single arginine in Escherichia coli ribosomal protein L7/L12 is close to 10(-3) for wild-type bacteria, decreases to 4 x 10(-4) in streptomycin-resistant bacteria containing mutant S12 (rpsL), and is virtually unchanged in Ram bacteria containing mutant S4 (rpsD) [7].
  • Interaction between mutations of ribosomes and RNA polymerase: a pair of strA and rif mutants individually temperature-insensitive but temperature-sensitive in combination [8].
  • Introduction of the nonpermissive rif allele to the permissive strA strain reduces or abolishes the transcription of T7 genome [6].
  • We have recently shown that single-base frameshifts were predominant among mutations induced within the rpsL target sequence upon oriC plasmid DNA replication in vitro [9].
  • Using single-stranded circular DNA containing either the coding sequence of the rpsL gene or its complementary sequence, the +1 frameshift mutagenesis by DNA polymerase III holoenzyme of Escherichia coli was extensively examined [9].

Chemical compound and disease context of rpsL


Biological context of rpsL


Anatomical context of rpsL

  • Streptomycin, which stimulates error production by blocking proofreading in vitro, did not increase efficiency of suppressor tRNAs in strains with normal or streptomycin-resistant (rpsL) ribosomes [16].
  • The membrane permeabilization was not due to a direct interaction of the antibiotic with the cell membrane, since cells that carry an rpsL mutation and synthesize proteins in a streptomycin-insensitive way did not lose K+ after the addition of the antibiotic [17].

Associations of rpsL with chemical compounds

  • The site of mutation is in the streptomycin (strA) region and is closely linked to the spcA gene [18].
  • This might explain why the expression of the lactose operon on rpsL strains remains coordinated when the intracellular level of formylation is decreased [19].
  • Therefore, the site preference of MNNG-induced rpsL- mutations seems to be due not to the specificity of methyl-transferring repair enzymes but probably to the distribution of the mutagenic lesions (O6-methylguanine) in the target sequence [4].
  • All of the mutations we characterized were localized at codons 43 or 88 of the rpsL gene and were base transitions from A to G, replacing lysine with arginine [3].
  • Under these conditions, 78% of mutations were induced at the central guanine of 5'-GG(A or C)-3' and 2/3 of them were on the non-transcribed strand of the rpsL gene [4].

Other interactions of rpsL


Analytical, diagnostic and therapeutic context of rpsL


  1. Modelling in Escherichia coli of mutations in mitoribosomal protein S12: novel mutant phenotypes of rpsL. Toivonen, J.M., Boocock, M.R., Jacobs, H.T. Mol. Microbiol. (1999) [Pubmed]
  2. Suppressors of temperature-sensitive mutations in a ribosomal protein gene, rpsL (S12), of Escherichia coli K12. Nashimoto, H., Miura, A., Saito, H., Uchida, H. Mol. Gen. Genet. (1985) [Pubmed]
  3. Spontaneous mutations in the Helicobacter pylori rpsL gene. Torii, N., Nozaki, T., Masutani, M., Nakagama, H., Sugiyama, T., Saito, D., Asaka, M., Sugimura, T., Miki, K. Mutat. Res. (2003) [Pubmed]
  4. Roles of transcription and repair in alkylation mutagenesis. Ito, T., Nakamura, T., Maki, H., Sekiguchi, M. Mutat. Res. (1994) [Pubmed]
  5. Expression of ribosomal protein genes in Escherichia coli. Cabezón, T., Faelen, M., De Wilde, M., Bollen, A., Thomas, R. Mol. Gen. Genet. (1975) [Pubmed]
  6. A link between streptomycin and rifampicin mutation. Chakrabarti, S.L., Gorini, L. Proc. Natl. Acad. Sci. U.S.A. (1975) [Pubmed]
  7. Codon-specific missense errors in vivo. Bouadloun, F., Donner, D., Kurland, C.G. EMBO J. (1983) [Pubmed]
  8. Interaction between mutations of ribosomes and RNA polymerase: a pair of strA and rif mutants individually temperature-insensitive but temperature-sensitive in combination. Chakrabarti, S.L., Gorini, L. Proc. Natl. Acad. Sci. U.S.A. (1977) [Pubmed]
  9. Strand asymmetry of +1 frameshift mutagenesis at a homopolymeric run by DNA polymerase III holoenzyme of Escherichia coli. Seki, M., Akiyama, M., Sugaya, Y., Ohtsubo, E., Maki, H. J. Biol. Chem. (1999) [Pubmed]
  10. Influence of sublethal concentrations of antibiotics on the expression of the mannose-specific ligand of Escherichia coli. Eisenstein, B.I., Beachey, E.H., Ofek, I. Infect. Immun. (1980) [Pubmed]
  11. Streptomycin resistance (rpsL) produces an absolute requirement for polyamines for growth of an Escherichia coli strain unable to synthesize putrescine and spermidine [delta(speA-speB) delta specC]. Tabor, H., Tabor, C.W., Cohn, M.S., Hafner, E.W. J. Bacteriol. (1981) [Pubmed]
  12. Mutations determining generalized resistance to aminoglycoside antibiotics in Escherichia coli. Thorbjarnardóttir, S.H., Magnúsdóttir, R.A., Eggertsson, G. Mol. Gen. Genet. (1978) [Pubmed]
  13. Genetics of ribosomal protein methylation in Escherichia coli. III. Map position of two genes, prmA and prmB, governing methylation of proteins L11 and L3. Colson, C., Lhoest, J., Urlings, C. Mol. Gen. Genet. (1979) [Pubmed]
  14. Prevalence of recombinational versus mutational events in damaged plasmid DNA containing regions of homology with the chromosome. Dolzani, L., Lagatolla, C., Monti-Bragadin, C. Mutat. Res. (1991) [Pubmed]
  15. Alteration of ribosomal proteins in revertants of a valyl-tRNA synthetase mutant of Escherichia coli. Wittmann, H.G., Stöffler, G. Mol. Gen. Genet. (1975) [Pubmed]
  16. Is efficiency of suppressor tRNAs controlled at the level of ribosomal proofreading in vivo? Faxén, M., Kirsebom, L.A., Isaksson, L.A. J. Bacteriol. (1988) [Pubmed]
  17. The bactericidal action of streptomycin: membrane permeabilization caused by the insertion of mistranslated proteins into the cytoplasmic membrane of Escherichia coli and subsequent caging of the antibiotic inside the cells due to degradation of these proteins. Busse, H.J., Wöstmann, C., Bakker, E.P. J. Gen. Microbiol. (1992) [Pubmed]
  18. Suppression of spectinomycin resistance in a mutant of Escherichia coli K-12. Berg, P.E., Kang, S.S. J. Bacteriol. (1975) [Pubmed]
  19. Formylation of initiator tRNA methionine in procaryotic protein synthesis: in vivo polarity in lactose operon expression. Petersen, H.U., Joseph, E., Ullmann, A., Danchin, A. J. Bacteriol. (1978) [Pubmed]
  20. How do combinations of rpsL- and miaA- generate streptomycin dependence? Diaz, I., Ehrenberg, M., Kurland, C.G. Mol. Gen. Genet. (1986) [Pubmed]
  21. Hyper-regulation of pyr gene expression in Escherichia coli cells with slow ribosomes. Evidence for RNA polymerase pausing in vivo? Jensen, K.F. Eur. J. Biochem. (1988) [Pubmed]
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