Disease relevance of ADRM1

• Monoclonal antibodies to gp110 and gp41 of human immunodeficiency virus [1].
• Decreased T cell precursor frequencies to Epstein-Barr virus glycoprotein Gp110 in peripheral blood correlate with disease activity and severity in patients with rheumatoid arthritis [2].

High impact information on ADRM1

• Following binding, Adrm1 relieves Uch37 autoinhibition, accelerating the hydrolysis of ubiquitin-7-amido-4-methylcoumarin (ubiquitin-AMC) [3].
• Recent work in our laboratory has demonstrated the selective depletion of a surface glycoprotein of 110,000 Mr (GP110) from LJP neutrophils by using surface labeling with [14C]formaldehyde and autofluorography [4].
• To quantify GP110 on LJP and normal neutrophils, fluorescein-labeled NCD-1 was bound to neutrophils and the amount of fluorescence was evaluated by using cytofluorography [4].
• Reduction of C5a and of FMLP ligand binding, decreased expression of GP-110, and reduced neutrophil chemotaxis are consistent with a stem cell maturation error in LJP patients [5].
• Results suggest that reduction of GP110 and FMLP receptor on neutrophils is specific for LJP patients who exhibit neutrophil chemotaxis abnormalities [6].

Biological context of ADRM1

• Adrm1 (human Rpn13, hRpn13) binds the carboxy-terminal tail of Uch37, a region that is distinct from the UCH catalytic domain, which we show inhibits Uch37 activity [3].

Anatomical context of ADRM1

• Adhesion properties of adhesion-regulating molecule 1 protein on endothelial cells [7].
• In this study, we show the presence of ARM-1 in endothelial cells, the adhesion partners of lymphocytes [7].
• Adhesion-regulating molecule-1 (ARM-1) is an adhesion-regulating molecule previously identified on T cells [7].
• We also show that ARM-1 is a cytosolic protein associated with the plasma membrane [7].
• Other patients with neutrophil defects express normal quantities of GP110, suggesting disease specificity [4].

Associations of ADRM1 with chemical compounds

• Five acidic glycoproteins iodinated in all lines by the lactoperoxidase/125I method were designated CP-175/5.8-6.0 (apparent molecular weight X 10(-3)/pl of iodoprotein), GP-155/5.0-5.3, GP-145/4.9-5.2, GP-130/4.8-5.5 and GP-110/4.9-5 [8].

Physical interactions of ADRM1

• Thus in human 26S proteasomes, hRpn13 appears to be important for the binding of UCH37 to the 19S complex and for efficient proteolysis [9].

Analytical, diagnostic and therapeutic context of ADRM1

• A serologic profile of antibody to GP110, P18, and P25 LAV/HTLV III antigens by radioimmunoprecipitation assay was also performed [10].
• We used the antibodies in indirect immunofluorescence assays to evaluate 13 clinical isolates of human immunodeficiency virus from diverse geographic regions, and we found that the gp110 epitope was recognized on all tested isolates, whereas the two gp41 epitopes were detected on 10 of 13 and 4 of 13 isolates [1].
• GP110 and FMLP receptors were quantified by flow cytometry on neutrophils from LJP patients with neutrophil chemotaxis defects, LJP patients without chemotaxis defects, other patients with primary neutrophil chemotaxis defects, and controls [6].

References