High impact information on P2rx5

• ATP regulates the differentiation of mammalian skeletal muscle by activation of a P2X5 receptor on satellite cells [1].
• Two new P2X receptor cDNAs (P2X5 and P2X6) were isolated and expressed [2].
• Rat P2X1 and P2X5 mRNAs are localized within common subsets of peripheral and central sensory neurons as well as spinal motoneurons [3].
• On the other hand, alpha,beta-methylene-ATP, beta,gamma-methylene-ATP, and UTP were unable to induce any rise in [Ca2+]i. This pharmacological profile is consistent with expression of P2X2 and/or P2X5 purinergic receptor channels [4].
• Conversely, ENaC increased the plasma membrane expression of P2X2, P2X(2/6), and P2X5 receptors [5].

Anatomical context of P2rx5

• P2X5 mRNA transcripts are found predominantly in rat heart but are also present in brain, spinal cord and adrenal gland [6].
• P2X5 immunoreactivity, a marker for differentiating cells in stratified squamous epithelia, was observed in the epithelia of the terminal urethra, the "horny spur" (spine-studded epithelium of the glans) and the inner surface of the prepuce [7].
• Especially prominent were large numbers of small clusters (about 400 nm diameter) of co-localised P2X2 and P2X5 receptors on the sarcolemma unrelated to nerves at all [8].
• P2X1,2 and 4 receptors were detected in vascular smooth muscle, while P2X3 receptors appeared to be associated with endothelial cells; some small arteries were positive for P2X5, possibly labelling vascular smooth muscle or fibroblasts in the adventitia [9].
• The relative occurrence of immunoreactive cortical cells of the zona fasciculata was highest for P2X6, followed by P2X7 and then P2X5 [10].

Associations of P2rx5 with chemical compounds

• Similar calcium, conductance, and capacitance responses could be activated by 2-methylthio-ATP and ADP, consistent with mediation by a P2 type purinergic receptor [11].

Other interactions of P2rx5

• During development the 1 day-old heart possessed an abundance of co-localised P2X2 and P2X5 small receptor clusters on the sarcolemma [8].
• A small number of chromaffin cells showed positive immunoreaction for P2X5 and P2X7, with the relative occurrence of P2X7-immunoreactive chromaffin cells exceeding that of P2X5 [10].
• Granular and microglial cells were labeled for P2X5 (weakly) and P2X4 receptors, respectively [12].

Analytical, diagnostic and therapeutic context of P2rx5

• We identified the P2X subtypes present in BAECs using RT-PCR. mRNA was present for only three of seven family members: P2X4, P2X5, and P2X7 [13].
• Localisation of P2X5 and P2X7 receptors by immunohistochemistry in rat stratified squamous epithelia [14].

References