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Alppl2  -  alkaline phosphatase, placental-like 2

Mus musculus

Synonyms: Akp5, Alkaline phosphatase 5, Alkaline phosphatase, placental-like, C77216, D1Ertd816e, ...
 
 
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Disease relevance of Akp5

  • An expression system was constructed for the production of E. coli alkaline phosphatase (EAP) labeled biscFv-6w10 (biscFv-6w10-EAP) in E. coli cells [1].
 

High impact information on Akp5

  • The Rb-1434 antibody enabled us to examine immunohistochemically what cell types in the testis are responsible for the expression of EAP during different developmental stages [2].
  • We have recently cloned and characterized a novel embryonic alkaline phosphatase (EAP) expressed at the two-cell to blastocyst stage of preimplantation development in the mouse [2].
  • Positive cells were first observed at day 15 and, at 24 days of age, many positive M-phase cells, morphologically corresponding to spermatocytes in mid to late prophase of meiotic division I, were strongly positive for EAP expression [2].
  • Furthermore, there was no compensation for the absence of EAP gene in EAP.ko embryos by other isozymes of alkaline phosphatase [3].
  • To determine whether the absence of an EAP gene affects development of preimplantation embryos, we studied mice homozygous for the disrupted EAP gene (EAP.ko mice) [3].
 

Biological context of Akp5

 

Anatomical context of Akp5

 

Associations of Akp5 with chemical compounds

  • Cyclic voltammetry of ascorbic acid, AZQ, and adriamycin in Hank's Balanced Salt Solution (HBSS pH 7.5), the buffer used for biological measurements, showed that the oxidation peak potential for ascorbic acid (Eap = +0.43 V) is closer to the reduction peak potential for AZQ (Ecp = -0.36 V) than that for adriamycin (Ecp = -0.67 V) [7].
 

Other interactions of Akp5

  • We report the inactivation, via homologous recombination, of two of the three active mouse alkaline phosphatase genes, i.e., embryonic (EAP) and tissue nonspecific (TNAP) [4].
 

Analytical, diagnostic and therapeutic context of Akp5

  • In the present report, we find that EAP transcripts can be detected, by RT-PCR analysis, in very low amounts in the testes of newborn mice, but at 24 days of age EAP mRNA levels reach the highest concentrations, remaining high at 40 and even 117 days of age [2].
  • We produced a synthetic peptide and a corresponding rabbit anti-peptide antiserum (Rb-1434), which was characterized by enzyme antigen immunoassays and reactivity with chinese hamster ovary cell transfectants, as reacting specifically with EAP [2].
  • Detections of PA and bacterial cells of B. anthracis using biscFv-6w10-EAP and Cy3-labeled biscFv-6w10 were performed on a protein chip [1].

References

  1. Construction of single chain variable fragment (ScFv) and BiscFv-alkaline phosphatase fusion protein for detection of Bacillus anthracis. Wang, S.H., Zhang, J.B., Zhang, Z.P., Zhou, Y.F., Yang, R.F., Chen, J., Guo, Y.C., You, F., Zhang, X.E. Anal. Chem. (2006) [Pubmed]
  2. Embryonic alkaline phosphatase is expressed at M-phase in the spermatogenic lineage of the mouse. Narisawa, S., Hofmann, M.C., Ziomek, C.A., Millán, J.L. Development (1992) [Pubmed]
  3. Effects of disruption of the embryonic alkaline phosphatase gene on preimplantation development of the mouse. Dehghani, H., Narisawa, S., Millán, J.L., Hahnel, A.C. Dev. Dyn. (2000) [Pubmed]
  4. Inactivation of two mouse alkaline phosphatase genes and establishment of a model of infantile hypophosphatasia. Narisawa, S., Fröhlander, N., Millán, J.L. Dev. Dyn. (1997) [Pubmed]
  5. Isolation and characterization of differentially expressed genes in invasive and non-invasive immortalized murine male germ cells in vitro. Tascou, S., Nayernia, K., Uedelhoven, J., Böhm, D., Jalal, R., Ahmed, M., Engel, W., Burfeind, P. Int. J. Oncol. (2001) [Pubmed]
  6. Developmental expression of alkaline phosphatase genes; reexpression in germ cell tumours and in vitro immortalized germ cells. Hofmann, M.C., Millán, J.L. Eur. Urol. (1993) [Pubmed]
  7. The influence of ascorbic acid on the free-radical metabolism of xenobiotics: the example of diaziquone. Gutierrez, P.L. Drug Metab. Rev. (1988) [Pubmed]
 
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