Disease relevance of Anp32a

• Alternative use of genes of the closely-related pp32 family is a common occurrence in human prostate cancer. pp32r1 and pp32r2, the oncogenic members of the pp32 family, are expressed in prostatic adenocarcinoma, while adjacent benign prostate continues to express pp32 [1].

Psychiatry related information on Anp32a

• The suitability of this novel screening system was established by the selection of mAbs recognising Alzheimer's disease (AD)-neurofibrillary pathology in histological preparations and phosphoprotein pp32 in rat brain sections [2].

High impact information on Anp32a

• In vivo colocalization and transfection studies show that pp32 INHAT domains are responsible for histone binding, HAT inhibitory activity, and repression of transcription [3].
• Regulation of histone acetylation and transcription by nuclear protein pp32, a subunit of the INHAT complex [3].
• The C-terminal third is exceptionally acidic, comprised of approximately 70% aspartic and glutamic acid residues; the predicted pI of human pp32 is 3.81 [4].
• Upon B cell stimulation the associated proteins are phosphorylated, giving rise to pp32/33 (alpha), pp37 (beta), and pp34 (previously designated gamma) [5].
• Both pp35 and pp32 appear to be associated with cell proliferation [6].

Biological context of Anp32a

• We demonstrate that antisense inhibition of pp32 in NIH3T3 cells leads to a variety of phenotypic changes associated with transformation including reduced serum dependence and loss of contact inhibition [1].
• Inhibition of T cell activation correlates with the ability of IgM and of cross-linked IgG anti-CD4 antibodies to induce tyrosine phosphorylation of the CD4-associated tyrosine kinase p56lck and an unknown substrate, pp32 [7].

Anatomical context of Anp32a

• Quantitative immunoblotting showed that both pp35 and pp32 are, respectively, expressed at 5.9 x 10(4) and 7.0 x 10(4) copies/cell in small, dense resting B lymphocytes, increasing approximately 12- and 7-fold after polyclonal stimulation with lipopolysaccharide [6].
• Two nuclear phosphoproteins, pp35 and pp32, were purified from A20 cells, a murine B-lymphoblastoid cell line [6].
• Antibodies specific for pp35 and pp32 show prominent intranuclear staining in A20 cells but only focal staining in normal murine lymphoid tissues [6].
• Overexpression of GFP-I1PP2A/lanp in PC12 cells leads to markedly reduced neurite length on laminin 5 after induction with nerve growth factor [8].

Associations of Anp32a with chemical compounds

• The phosphatase binds to the cytoplasmic membrane-proximal conserved GFFKR motif of the alpha integrin subunit, whereas I1PP2A/lanp requires a longer sequence for binding [8].
• The proteins pp32 and pp30 have apparent isoelectric points of 6.6 and 6.5 and molecular weights of approximately 32 30 kDa respectively, as determined by two-dimensional polyacrylamide gel electrophoresis [9].

Analytical, diagnostic and therapeutic context of Anp32a

• By two-dimensional peptide mapping, pp35 and pp32 are related but do not appear to represent sequential proteolytic products [6].

References