Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Gene Review:

mutS - DNA mismatch repair protein MutS

Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

Zahrt, T.C. et al., Lee, C.A. et al., Le, O. et al., Haber, L.T. et al., Campoy, S. et al., et al.

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of mutS

Although the disease progression is slower, S. typhimurium mutS recD mutants retain the ability to cause lethal infections, and, thus, hybrids constructed in mutS recD hosts may permit the analysis of virulence factors in a surrogate animal model [1].
An Azotobacter vinelandii homolog to the Salmonella typhimurium mutS gene was discovered upstream of the fdxA gene [2].
Azotobacter vinelandii mutS: nucleotide sequence and mutant analysis [2].
Nucleotide sequence of the Salmonella typhimurium mutS gene required for mismatch repair: homology of MutS and HexA of Streptococcus pneumoniae [3].
One of the strains carries a null mutS mutation, while the other harbors plasmid pRW30, which contains the Escherichia coli umuDC operon [4].

High impact information on mutS

The results suggest that hil encodes an invasion factor or an activator of invasion factor expression. hil maps between srl and mutS near minute 59.5 of the S. typhimurium chromosome, a region adjacent to other loci that have been identified as required for S. typhimurium invasiveness and virulence [5].
We also show that in mutS mutants, msDNA does not increase the frequency of either mutations or interspecies recombination [6].
Recipients with mutS and recD mutations overcome this barrier, allowing construction of genetic hybrids [1].
This phenotype results from an inability of mutS recD double mutants to rapidly adapt to purine-limiting conditions present within macrophages [1].
Furthermore, transduction of mutS and mutL derivatives of S. typhi with chromosomal genes from S. typhimurium occurs efficiently [7].

Biological context of mutS

We have characterized the mutS-proximal border of this pathogenicity island and have identified two sets of genes, pigAB and pigCD [8].
This A/G mismatch repair is independent of ATP, dam methylation, and mutS gene function [9].

Other interactions of mutS

Mutants defective in either mutS or recD do not affect the time course or the 50% lethal dose (LD(50)) of the infection [1].
The invA locus was found to be located at about 59 min on the Salmonella chromosome, 7% linked to mutS [10].

References

Effect of mutS and recD mutations on Salmonella virulence. Zahrt, T.C., Buchmeier, N., Maloy, S. Infect. Immun. (1999) [Pubmed]
Azotobacter vinelandii mutS: nucleotide sequence and mutant analysis. Le, O., Shen, B., Iismaa, S.E., Burgess, B.K. J. Bacteriol. (1993) [Pubmed]
Nucleotide sequence of the Salmonella typhimurium mutS gene required for mismatch repair: homology of MutS and HexA of Streptococcus pneumoniae. Haber, L.T., Pang, P.P., Sobell, D.I., Mankovich, J.A., Walker, G.C. J. Bacteriol. (1988) [Pubmed]
Virulence and mutation rates of Salmonella typhimurium strains with increased mutagenic strength in a mouse model. Campoy, S., Pérez de Rozas, A.M., Barbé, J., Badiola, I. FEMS Microbiol. Lett. (2000) [Pubmed]
Identification of a Salmonella typhimurium invasion locus by selection for hyperinvasive mutants. Lee, C.A., Jones, B.D., Falkow, S. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
Multicopy single-stranded DNA of Escherichia coli enhances mutation and recombination frequencies by titrating MutS protein. Maas, W.K., Wang, C., Lima, T., Hach, A., Lim, D. Mol. Microbiol. (1996) [Pubmed]
Inactivation of mismatch repair overcomes the barrier to transduction between Salmonella typhimurium and Salmonella typhi. Zahrt, T.C., Mora, G.C., Maloy, S. J. Bacteriol. (1994) [Pubmed]
Characterization of the mutS-proximal region of the Salmonella typhimurium SPI-1 identifies a group of pathogenicity island-associated genes. Pancetti, A., Galán, J.E. FEMS Microbiol. Lett. (2001) [Pubmed]
Specific A/G-to-C.G mismatch repair in Salmonella typhimurium LT2 requires the mutB gene product. Lu, A.L., Cuipa, M.J., Ip, M.S., Shanabruch, W.G. J. Bacteriol. (1990) [Pubmed]
Molecular and functional characterization of the Salmonella invasion gene invA: homology of InvA to members of a new protein family. Galán, J.E., Ginocchio, C., Costeas, P. J. Bacteriol. (1992) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.