Disease relevance of AMOT

• RESULTS: Breast cancer tissues expressed significantly higher levels of angiomotin transcript, compared with normal mammary tissues (33.1 +/- 11 in normal versus 86.5 +/- 13.7 in tumour tissues, p = 0.003) [1].

High impact information on AMOT

• A Rich1/Amot Complex Regulates the Cdc42 GTPase and Apical-Polarity Proteins in Epithelial Cells [2].
• We propose that Rich1 and Amot maintain TJ integrity by the coordinate regulation of Cdc42 and by linking specific components of the TJ to intracellular protein trafficking [2].
• Hold that line. Angiomotin regulates endothelial cell motility [3].
• Expression of angiomotin in endothelial cells resulted in increased cell migration, suggesting a stimulatory role of angiomotin in cell motility [4].
• Angiomotin: an angiostatin binding protein that regulates endothelial cell migration and tube formation [4].

Biological context of AMOT

• These findings indicate that angiostatin inhibits cell migration by interfering with angiomotin activity in endothelial cells [4].
• Here we show that the putative PDZ binding motif of angiomotin serves as a protein recognition site and that deletion of three amino acids in this site results in inhibition of chemotaxis [5].
• Transfection of p130-angiomotin into endothelial cells induces actin fiber formation and changes cell shape [6].
• The levels of expression of angiomotin, L1 and L2 were determined using reverse transcription PCR [1].

Anatomical context of AMOT

• In vivo, angiomotin is expressed in the endothelial cells of capillaries as well as larger vessels of the human placenta [4].
• Upon expression of angiomotin in HeLa cells, angiomotin bound and internalized fluorescein-labeled angiostatin [4].
• Immunofluorescence analysis shows that angiomotin co-localized with ZO-1 in cell-cell contacts in endothelial cells in vitro and in angiogenic blood vessels of the postnatal mouse retina in vivo [7].

Associations of AMOT with chemical compounds

• To study the effect of angiomotin on embryonic angiogenesis, we generated transgenic mice expressing wild-type angiomotin and the C-terminal deletion mutant driven by the endothelial cell-specific receptor tyrosine kinase (TIE) promoter [5].

Other interactions of AMOT

• This study examined the expression of angiomotin and its analogues, angiomotin-like 1 (L1) and -like 2 (L2) in breast tumour tissues, and analysed their correlation with angiogenesis and clinical outcomes [1].

Analytical, diagnostic and therapeutic context of AMOT

• Here, we describe a strategy that targets the angiostatin receptor angiomotin (Amot) by DNA vaccination [8].

References