Disease relevance of EEF1G

• Expression of elongation factor-1 gamma-related sequence in human pancreatic cancer [1].
• Expression of an elongation factor 1 gamma-related sequence in adenocarcinomas of the colon [2].
• Overexpression of an elongation factor-1 gamma-hybridizing RNA in colorectal adenomas [3].
• Elongation factor 1 gamma (EF1 gamma) has been isolated from a pancreatic cancer cell line, while the overexpression of EF1 gamma has also been observed in colorectal carcinomas and adenomas [4].
• The authors examined the expression of EF1 gamma mRNA in 30 gastric carcinomas and their corresponding paired adjacent normal mucosae obtained during surgery while also examining 10 human digestive tract carcinoma cell lines using Northern blot hybridization [4].

High impact information on EEF1G

• The authors have previously reported that a messenger RNA (mRNA) bearing 60% homology to elongation factor 1 gamma in Artemia salina was overexpressed in 7 of 9 pancreatic tumors relative to normal appearing adjacent tissue [2].
• All 15 oesophageal carcinoma cells expressed EF1 gamma mRNA relatively lower than the gastric or pancreatic carcinoma cell lines, in which EF1 gamma was originally isolated [5].
• Elongation factor 1 gamma (EF1 gamma) is known to be a subunit of EF1, one of the G proteins that mediate the transport of aminoacyl tRNA to 80S ribosomes during translation [5].
• Nucleotide sequence of a Trypanosoma cruzi cDNA encoding a protein homologous to mammalian EF1 gamma [6].
• Human cDNAs encoding elongation factor 1 gamma and the ribosomal protein L19 [7].

Biological context of EEF1G

• The deduced amino acid sequence shared 53% identity with the amino acid sequence for the Artemia elongation factor-1 gamma [1].
• A computer search of the Intelligenetics System of all available nucleotide sequences revealed a 60% homology between the nucleotide sequence of the pPDC-1 cDNA and that of elongation factor-1 gamma from Artemia [1].
• Further sequence analysis of cDNA clones, originating from this particular genomic region, confirmed the existence of an open reading frame with a short intron, which encodes a 409-aa protein with striking homology to eukaryotic elongation factor-1 gamma [8].
• A small portion of genomic EF-1gamma that could be amplified from araneomorphs contained a short intron, suggesting that longer genomic sequences could not be amplified due to the presence of introns [9].
• This paper reports the isolation and characterization of a complementary deoxyribonucleic acid clone showing sequence homology with genes coding for the eukaryotic elongation factor 1 gamma (EF-1 gamma) [10].

Anatomical context of EEF1G

• We concluded that EF-1 gamma is a substrate for MPF during oocyte maturation in goldfish [11].

Associations of EEF1G with chemical compounds

• Eukaryotic translation elongation factor 1 gamma contains a glutathione transferase domain--study of a diverse, ancient protein superfamily using motif search and structural modeling [12].

Other interactions of EEF1G

• GST-like proteins related to class theta comprise a large group including, in addition to typical GSTs and EF1 gamma, stress-induced proteins from bacteria and plants, bacterial reductive dehalogenases and beta-etherases, and several uncharacterized proteins [12].
• A polyvalent antiserum against EF-1 beta and two monoclonal antibodies against EF-1 gamma were used to identify the protein fragments in this core [13].
• The affinity of EF-1 alpha for other subunits was as follows, beta beta' gamma > beta beta' > beta not equal to beta'. Likewise, the affinity of EF-1 gamma for other subunits was: beta beta' gamma > beta >> beta'. Phe-tRNA binding activity of the reconstructed EF-1 was about 90% of that of the native EF-1 [14].

Analytical, diagnostic and therapeutic context of EEF1G

• As little is known regarding the expression of EF1 gamma in human oesophageal carcinoma, this study looked at its expression using a northern blot analysis [5].
• Southern hybridization and pulsed field gel electrophoresis showed that EF-1 gamma genes are highly conserved among members of the Leishmania genus and must be clustered in a single chromosomal locus [10].

References