High impact information on igf1rb

• In particular, the relative level of IGF-IRa mRNA was low during early embryogenesis and increased in the hatched larva, whereas the situation was reversed for IGF-IRb mRNA [1].
• By RT-PCR, zebrafish IGF-IR expression was detected from early blastula to early larval stages of development [2].
• To understand the relationship of IGF-IR with p53 suppressor gene during embryogenesis, expression of both genes was analyzed in parallel by semicompetitive reverse transcriptase PCR and whole-mount in situ hybridization [3].
• Besides being mitogenic, IGF-IR plays a crucial role in cell survival, transformation and maintenance of the malignant phenotype [4].
• The level of IGF-IR mRNA expression was much higher in retina, gonad, skeletal muscle and gill tissues than in liver, heart and thymus tissues [4].

Biological context of igf1rb

• The differential expression pattern of IGF-IRa and IGF-IRb suggest that they may play distinct roles in regulating the growth and development of zebrafish [1].
• Comparison of the amino acid sequence with those of other species showed that the grouper IGF-IR shares 90.2%, 89.6%, 71.9% and 72% similarity with the IGF-IR of the Japanese flounder, turbot, zebrafish-a and zebrafish-b, respectively [4].

Anatomical context of igf1rb

• The expression of IGF-IR can be visualized as a ubiquitous signal in unfertilized eggs, embryos and early development larvae [4].

Analytical, diagnostic and therapeutic context of igf1rb

• Immunoprecipitation studies with specific antibodies showed that both IGF-IR genes are expressed and both receptors bind to IGFs and des(1-3)IGF-I, but not to insulin [1].
• Using whole mount in situ hybridization, IGF-IR expression was detected after gastrulation [2].
• To understand the functions of IGF-IR in zebrafish embryogenesis, the polymerase chain reaction (PCR) cloning technique was applied to isolate the IGF-IR gene [3].

References