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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Gene Review: 

Gpc3  -  glypican 3

Rattus norvegicus

Synonyms: Glypican-3, Intestinal protein OCI-5
 
 
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High impact information on Gpc3

  • Clone OCI-5 detected homologous sequences in human and murine cells [1].
  • The sequence of clone OCI-5 showed an open reading frame that was capable of encoding a protein of 597 amino acids, but no strong homology was found with any of the proteins registered in the protein sequence data base [1].
  • When IEC-18 cells were transformed by activated H-ras or v-src genes, expression of clone OCI-5 was suppressed; the degree of down-regulation correlated with the extent of morphological change induced in the transformed IEC-18 cells [1].
  • Clone OCI-5 was selected from the rat intestinal cell line IEC-18, which represents primitive intestinal epithelial crypt cells [1].
  • Nucleotide sequence analysis revealed that this transcript is encoded by the rat glypican 3 gene (GPC3), whose human homolog is mutated in the Simpson-Golabi-Behmel overgrowth syndrome [2].
 

Biological context of Gpc3

  • Together, these data suggest that OCI-5 expression is regulated in IEC-18 by cell shape [3].
  • OCI-5, the rat homologue of human glypican 3 (GPC3), is believed to be involved in morphogenesis and growth control during development [3].
  • The present study was undertaken to detect gene expression change of OCI-5 during occurrence and progression of rat HCC [4].
  • Gradual upregulation of OCI-5 expression during occurrence and progression of rat hepatocellular carcinoma [4].
 

Anatomical context of Gpc3

  • This cDNA, named OCI-5, was recently shown to have 20-25% identity at the protein-sequence level with glypican and cerebroglycan, two heparan sulphate proteoglycans (HSPG) that are attached to the cell membrane by a glycosyl-phosphatidylinositol (GPI) anchor [5].
  • We also show that treatment with phosphatidylinositol-specific phospholipase C releases OCI-5 from the cell surface of COS cells transfected with an OCI-5 expression vector [5].
  • To investigate the mechanism of its regulation during intestinal development, OCI-5 expression was studied in the primitive rat intestinal epithelial cell line IEC-18 [3].
 

Associations of Gpc3 with chemical compounds

  • Treatment with vanadate, a phosphatase inhibitor, causes cells to acquire a spindle-shaped morphology and prevents OCI-5 induction in all situations [3].
 

Analytical, diagnostic and therapeutic context of Gpc3

  • In this report, using RNA in situ hybridization, expression of OCI-5 in the developing intestine is detected in both endoderm- and mesenchyme-derived cells in a phased manner related to age and proximal/distal position [3].
  • The gene expression levels of OCI-5 and GPC3 were detected with the RT-PCR method [4].

References

  1. Isolation of a cDNA corresponding to a developmentally regulated transcript in rat intestine. Filmus, J., Church, J.G., Buick, R.N. Mol. Cell. Biol. (1988) [Pubmed]
  2. Expression of GPC3, an X-linked recessive overgrowth gene, is silenced in malignant mesothelioma. Murthy, S.S., Shen, T., De Rienzo, A., Lee, W.C., Ferriola, P.C., Jhanwar, S.C., Mossman, B.T., Filmus, J., Testa, J.R. Oncogene (2000) [Pubmed]
  3. Expression of OCI-5/glypican 3 during intestinal morphogenesis: regulation by cell shape in intestinal epithelial cells. Li, M., Choo, B., Wong, Z.M., Filmus, J., Buick, R.N. Exp. Cell Res. (1997) [Pubmed]
  4. Gradual upregulation of OCI-5 expression during occurrence and progression of rat hepatocellular carcinoma. Jiang, W.J., Man, X.B., Tang, L., Song, H.Y., Li, S.J., Cai, G.J., Qiu, X.H., Hu, H.P. HBPD INT (2006) [Pubmed]
  5. Identification of a new membrane-bound heparan sulphate proteoglycan. Filmus, J., Shi, W., Wong, Z.M., Wong, M.J. Biochem. J. (1995) [Pubmed]
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