High impact information on HIST1H2BA

• Anti-H2B antibodies block interactions of hSTBP with telomere DNA, and spH2B forms specific complex with this DNA in vitro, indicating that this protein plays a role in telomere DNA recognition [1].
• We propose that hSTBP participates in the membrane attachment of telomeres that may be important for ordered chromosome withdrawal after fertilization [1].
• Human testis/sperm-specific histone H2B (hTSH2B). Molecular cloning and characterization [2].
• The H2B variant associated with telomere-binding activity () was solubilized by Triton X-100 or micrococcal nuclease extraction, whereas hTSH2B was relatively tightly bound in nuclei [2].
• We found that STBP is an integral protein bound to the endoplasmic reticulum [3].

Biological context of HIST1H2BA

• Novel human testis-specific histone H2B encoded by the interrupted gene on the X chromosome [4].
• We propose that the presence or absence of hTSH2B within spermatozoa influences pronuclei formation and the activation of paternal genes following fertilisation and during early embryonic development [5].

Anatomical context of HIST1H2BA

• The hTSH2B gene is transcribed exclusively in testis, and the corresponding protein is also present in mature sperm [2].
• We established that the presence of hTSH2B does not influence sperm zona pellucida binding capacity [5].

Associations of HIST1H2BA with chemical compounds

• The availability of solubilized STBP and the fact that it can be induced in vivo represent major steps toward the purification and functional significance of this unique steroid-binding protein [3].
• In this study we solubilized the male rat liver ST-binding protein (STBP) and investigated the following parameters: 1) pharmacological properties, 2) hydrodynamic properties, 3) peptidic composition, 4) the effects of age and hypophysectomy, and 5) inducibility by 17alpha-ethinyl estradiol [3].

Analytical, diagnostic and therapeutic context of HIST1H2BA

• Immunofluorescence showed that hTSH2B was concentrated in spots located at the basal nuclear area of a subpopulation (20% of cells) of mature sperm [2].
• Using genomic PCR, two genetic alleles of hTSH2B were found in the human population [2].
• STBP increased from immature to adult rats, and it dramatically decreased after hypophysectomy [3].
• SDS-PAGE of covalently labeled STBP under nonreducing conditions showed that [3H]ST binds to a 110-kDa protein [3].
• Using indirect immunofluorescence, we examined the influence of hTSH2B on zona pellucida binding and sperm head decondensation in amphibian egg cell-free extract [5].

References