Disease relevance of Ceacam2

• Bgp2, a new member of the carcinoembryonic antigen-related gene family, encodes an alternative receptor for mouse hepatitis viruses [1].
• Purified, soluble recombinant mouse hepatitis virus receptor, Bgp1(b), and Bgp2 murine coronavirus receptors differ in mouse hepatitis virus binding and neutralizing activities [2].

High impact information on Ceacam2

• The Bgp2 cDNA allowed the prediction of a 271-amino-acid glycoprotein with two immunoglobulin domains, a transmembrane, and a putative cytoplasmic tail [1].
• RNase protection assays and RNA PCR showed that Bgp2 was expressed in BALB/c kidney, colon, and brain tissue, in SJL/J colon and liver tissue, in BALB/c and CD1 spleen tissue, in C3H macrophages, and in mouse rectal carcinoma CMT-93 cells [1].
• There is considerable divergence in the amino acid sequences of the N-terminal domains of the proteins coded by the Bgp1 gene from that of the Bgp2-encoded protein [1].
• Reverse transcription-PCR using testis RNA indicated that Ceacam2 in the testis is an alternatively spliced form containing only exons 1, 2, 5, 6, 8 and 9 [3].
• On the other hand, no Ceacam2 mRNA was detected throughout embryonic development [3].

Chemical compound and disease context of Ceacam2

• When Bgp2-transfected hamster cells were challenged with MHV-A59, MHV-JHM, or MHV-3, the Bgp2-encoded protein served as a functional MHV receptor, although with a lower efficiency than that of the MHVR glycoprotein [1].

Biological context of Ceacam2

• While most of these differences were due to nucleotide substitutions, two insertions of 418 and 5849 bp occurred in intron 2 of Ceacam2, and another two insertions of 1384 and 197 bp occurred in introns 5 and 7 respectively [3].

Anatomical context of Ceacam2

• In contrast, Ceacam2 mRNA was only detected in kidney, testis and, to a lesser extent, spleen [3].
• The Bgp2 protein is expressed in low amounts in kidney and in a rectal carcinoma cell line [4].
• Bgp2 was found by immunocytochemistry in smooth muscle layers of the kidney, the uterus, in gut mononuclear cells and in the crypt epithelia of intestinal tissues [4].
• Both MHV-Y and -RI infected Vero cells transiently transfected with the Bgp2 receptor gene, though only MHV-Y infected CHO cells stably transfected with the Bgp2 receptor gene [5].

Other interactions of Ceacam2

• The Bgp2-mediated virus infection could not be inhibited by monoclonal antibody CC1 that is specific for the N-terminal domain of MHVR [1].

Analytical, diagnostic and therapeutic context of Ceacam2

• Our sequence analysis revealed that the genes encoded nine exons and spanned approx. 16-17 kb (Ceacam1) and 25 kb (Ceacam2) [3].

References