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Gene Review

Rnase1  -  ribonuclease, RNase A family, 1 (pancreatic)

Mus musculus

Synonyms: AI574248, RNase 1, RNase A, Rib-1, Rib1, ...
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Disease relevance of Rnase1

  • METHODS: After induction of trinitrobenzene sulfonic acid (TNBS) colitis in SJL/J mice, plasma hormone and cytokine levels were measured, adrenal structure was analyzed by immunohistochemistry and electron microscopy, and adrenal cytokine/cytokine receptor expression were studied by RNase protection [1].
  • When the truncated RNase was stably expressed to high levels in murine cells, it prevented specific rRNA cleavage in response to 2-5A transfection and the cells were unresponsive to the antiviral activity of interferon alpha/beta for encephalomyocarditis virus [2].
  • Characterization and mapping of RNase T1-resistant oligonucleotides derived from the genomes of Akv and MCF murine leukemia viruses [3].
  • To assess if Tat can carry other molecules into cells, we chemically cross-linked Tat peptides (residues 1-72 or 37-72) to beta-galactosidase, horseradish peroxidase, RNase A, and domain III of Pseudomonas exotoxin A (PE) and monitored uptake colorimetrically or by cytotoxicity [4].
  • Northern blot hybridization and RNase protection assay revealed that Cart-1 RNA was present at high levels in a well-differentiated rat chondrosarcoma tumor and in a cell line derived from this tumor [5].

High impact information on Rnase1

  • We have now used an RNase protection assay that can distinguish between H19 alleles in four subspecies of Mus, to demonstrate that the H19 gene is parentally imprinted, with the active copy derived from the mother [6].
  • RNase mitochondrial RNA processing correctly cleaves a novel R loop at the mitochondrial DNA leading-strand origin of replication [7].
  • A partial cDNA encoding AP-2 was isolated from a mouse embryo cDNA library and used to prepare probes to measure AP-2 mRNA levels by RNase protection and RNA in situ hybridization [8].
  • For two unrelated model antigens (RNase and horseradish peroxidase), we found that only the less digestible forms were immunogenic, inducing far more efficient T cell priming and antibody responses [9].
  • T cell lineage choice and differentiation in the absence of the RNase III enzyme Dicer [10].

Chemical compound and disease context of Rnase1

  • Using RNase protection assay for screening of the melanoma progression profile, and Western blotting for subsequent result validation, we looked for molecular progression markers affected by melanoma histamine secretion [11].
  • We have extensively purified from Krebs II ascites cells, although not until homogeneity, a ribonuclease which preferentially cleaves natural or synthetic double-stranded RNA substrates (RNase D); this specificity is also supported by its sensitivity to inhibition by 10(-5) M ethidium bromide [12].
  • Polyacrylamide gel electrophoretic analysis of the RNase-treated 40S structure revealed at least three viral structural proteins in the same ratio as was present in the intact virions [13].
  • Lipopolysaccharide (LPS) of Burkholderia cepacia was purified by the conventional phenol-water extraction method (preparation BcLPS-1), followed by enzymatic treatments with DNase, RNase, trypsin, and proteinase K (preparation BcLPS-2), and finally by deoxycholate-phenol-water extraction (preparation BcLPS-3) [14].
  • Poly ADP-ribosylation of two mouse lymphoma cell lines, L5178Y (LS) and the radiation and alkylating agent resistant derivative AII, was investigated by uptake of [3H]NAD by permeabilised cells into acid-precipitable material that was sensitive to phosphodiesterase but insensitive to DNase and RNase [15].

Biological context of Rnase1


Anatomical context of Rnase1


Associations of Rnase1 with chemical compounds

  • These nuclear synaptonemal complex-matrices are prepared by extracting Triton X-100-treated nuclei with low (0.2 M) and high (1.0 or 2.0 M) NaCl, DNase I, and RNase A to remove 85% of the nuclear proteins, 97% of the RNA, and 99% of the DNA [25].
  • The data indicate that no reiteration can be detected by RNase or by hydroxylapatite for the genes corresponding to the entire sequence of MOPC 41 L-chain mRNA, including the untranslated segments, within the limits of detectability of short reiterated segments [26].
  • Activation-induced cytidine deaminase deaminates deoxycytidine on single-stranded DNA but requires the action of RNase [27].
  • RNase protection and primer extension analyses revealed a single transcriptional start site located 299 bp upstream from the translational start site in an initiator-like pyrimidine-rich sequence [28].
  • RNase protection assays demonstrated that Epo or dimethyl sulfoxide induction increased steady-state mRNA levels 10- to 20-fold after 24 to 48 hours [29].

Physical interactions of Rnase1


Regulatory relationships of Rnase1


Other interactions of Rnase1

  • This and other evidence suggests that the RNase A superfamily originated from an RNase 5-like gene and expanded in mammals [35].
  • The data suggest that Rib-1 is transcribed in pancreas and parotid from the same promoter [21].
  • Three PurBPs of 35, 38 and 40 kDa were found mostly in the nuclear extract (N.Ext.) and they were not detected by the pretreatment of N.Ext. with trypsin, but not with RNase or DNase [36].
  • DSG3 -/- mice had no DSG3 mRNA by RNase protection assay and no Dsg3 protein by immunofluorescence (IF) and immunoblots [37].
  • In this study, we have used an RNase protection assay to demonstrate that IL-4 induces expression of germ-line gamma 1 transcripts in B cells within 4 hr of culture; induction is dose-dependent and is inhibited by interferon gamma [38].

Analytical, diagnostic and therapeutic context of Rnase1


  1. Adrenal cortical activation in murine colitis. Franchimont, D., Bouma, G., Galon, J., Wolkersdörfer, G.W., Haidan, A., Chrousos, G.P., Bornstein, S.R. Gastroenterology (2000) [Pubmed]
  2. A dominant negative mutant of 2-5A-dependent RNase suppresses antiproliferative and antiviral effects of interferon. Hassel, B.A., Zhou, A., Sotomayor, C., Maran, A., Silverman, R.H. EMBO J. (1993) [Pubmed]
  3. Characterization and mapping of RNase T1-resistant oligonucleotides derived from the genomes of Akv and MCF murine leukemia viruses. Rommelaere, J., Faller, D.V., Hopkins, N. Proc. Natl. Acad. Sci. U.S.A. (1978) [Pubmed]
  4. Tat-mediated delivery of heterologous proteins into cells. Fawell, S., Seery, J., Daikh, Y., Moore, C., Chen, L.L., Pepinsky, B., Barsoum, J. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  5. Cartilage homeoprotein 1, a homeoprotein selectively expressed in chondrocytes. Zhao, G.Q., Zhou, X., Eberspaecher, H., Solursh, M., de Crombrugghe, B. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  6. Parental imprinting of the mouse H19 gene. Bartolomei, M.S., Zemel, S., Tilghman, S.M. Nature (1991) [Pubmed]
  7. RNase mitochondrial RNA processing correctly cleaves a novel R loop at the mitochondrial DNA leading-strand origin of replication. Lee, D.Y., Clayton, D.A. Genes Dev. (1997) [Pubmed]
  8. Transcription factor AP-2 is expressed in neural crest cell lineages during mouse embryogenesis. Mitchell, P.J., Timmons, P.M., Hébert, J.M., Rigby, P.W., Tjian, R. Genes Dev. (1991) [Pubmed]
  9. Enhancing immunogenicity by limiting susceptibility to lysosomal proteolysis. Delamarre, L., Couture, R., Mellman, I., Trombetta, E.S. J. Exp. Med. (2006) [Pubmed]
  10. T cell lineage choice and differentiation in the absence of the RNase III enzyme Dicer. Cobb, B.S., Nesterova, T.B., Thompson, E., Hertweck, A., O'Connor, E., Godwin, J., Wilson, C.B., Brockdorff, N., Fisher, A.G., Smale, S.T., Merkenschlager, M. J. Exp. Med. (2005) [Pubmed]
  11. Phenotypic profiling of engineered mouse melanomas with manipulated histamine production identifies histamine H2 receptor and rho-C as histamine-regulated melanoma progression markers. Pós, Z., Sáfrány, G., Müller, K., Tóth, S., Falus, A., Hegyesi, H. Cancer Res. (2005) [Pubmed]
  12. Isolation and characterization of a ribonuclease activity specific for double-stranded RNA (RNase D) from Krebs II ascites cells. Rech, J., Cathala, G., Jeanteur, P. J. Biol. Chem. (1980) [Pubmed]
  13. Protein-RNA interaction in encephalomyocarditis virus as revealed by UV light-induced covalent linkages. Smirnov, Y.A., Rodrigues-Molto, M.P., Famadas, M.T. J. Virol. (1983) [Pubmed]
  14. Lipopolysaccharide of Burkholderia cepacia and its unique character to stimulate murine macrophages with relative lack of interleukin-1beta-inducing ability. Shimomura, H., Matsuura, M., Saito, S., Hirai, Y., Isshiki, Y., Kawahara, K. Infect. Immun. (2001) [Pubmed]
  15. Poly (ADP-ribose) metabolism in alkylated mouse L5178Y cells. Boyle, J.M. Carcinogenesis (1985) [Pubmed]
  16. Assignment of pancreatic ribonuclease gene to mouse chromosome 14. Elliott, R.W., Samuelson, L.C., Lambert, M.S., Meisler, M.H. Cytogenet. Cell Genet. (1986) [Pubmed]
  17. Detection and quantification of the leptin receptor splice variants Ob-Ra, b, and, e in different mouse tissues. Löllmann, B., Grüninger, S., Stricker-Krongrad, A., Chiesi, M. Biochem. Biophys. Res. Commun. (1997) [Pubmed]
  18. Secretion of insulinlike growth factor I and insulinlike growth factor-binding proteins by murine bone marrow stromal cells. Abboud, S.L., Bethel, C.R., Aron, D.C. J. Clin. Invest. (1991) [Pubmed]
  19. The beta-globin locus control region enhances transcription of but does not confer position-independent expression onto the lacZ gene in transgenic mice. Guy, L.G., Kothary, R., DeRepentigny, Y., Delvoye, N., Ellis, J., Wall, L. EMBO J. (1996) [Pubmed]
  20. Recombination within the upstream gene of duplicated myelin basic protein genes of myelin deficient shimld mouse results in the production of antisense RNA. Okano, H., Ikenaka, K., Mikoshiba, K. EMBO J. (1988) [Pubmed]
  21. Isolation of the murine ribonuclease gene Rib-1: structure and tissue specific expression in pancreas and parotid gland. Samuelson, L.C., Wiebauer, K., Howard, G., Schmid, R.M., Koeplin, D., Meisler, M.H. Nucleic Acids Res. (1991) [Pubmed]
  22. G1 arrest of U937 cells by onconase is associated with suppression of cyclin D3 expression, induction of p16INK4A, p21WAF1/CIP1 and p27KIP and decreased pRb phosphorylation. Juan, G., Ardelt, B., Li, X., Mikulski, S.M., Shogen, K., Ardelt, W., Mittelman, A., Darzynkiewicz, Z. Leukemia (1998) [Pubmed]
  23. Implication of TNF-related apoptosis-inducing ligand in inflammatory intestinal epithelial lesions. Begue, B., Wajant, H., Bambou, J.C., Dubuquoy, L., Siegmund, D., Beaulieu, J.F., Canioni, D., Berrebi, D., Brousse, N., Desreumaux, P., Schmitz, J., Lentze, M.J., Goulet, O., Cerf-Bensussan, N., Ruemmele, F.M. Gastroenterology (2006) [Pubmed]
  24. Interferon action and apoptosis are defective in mice devoid of 2',5'-oligoadenylate-dependent RNase L. Zhou, A., Paranjape, J., Brown, T.L., Nie, H., Naik, S., Dong, B., Chang, A., Trapp, B., Fairchild, R., Colmenares, C., Silverman, R.H. EMBO J. (1997) [Pubmed]
  25. Synaptonemal complexes are integral components of the isolated mouse spermatocyte nuclear matrix. Ierardi, L.A., Moss, S.B., Bellvé, A.R. J. Cell Biol. (1983) [Pubmed]
  26. No detectable reiteration of genes coding for mouse MOPC 41 immunoglobulin light-chain mRNA. Farace, M.G., Aellen, M.F., Briand, P.A., Faust, C.H., Vassalli, P., Mach, B. Proc. Natl. Acad. Sci. U.S.A. (1976) [Pubmed]
  27. Activation-induced cytidine deaminase deaminates deoxycytidine on single-stranded DNA but requires the action of RNase. Bransteitter, R., Pham, P., Scharff, M.D., Goodman, M.F. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  28. Characterization of the endothelium-specific murine vascular endothelial growth factor receptor-2 (Flk-1) promoter. Rönicke, V., Risau, W., Breier, G. Circ. Res. (1996) [Pubmed]
  29. Regulated expression and functional role of the transcription factor CHOP (GADD153) in erythroid growth and differentiation. Coutts, M., Cui, K., Davis, K.L., Keutzer, J.C., Sytkowski, A.J. Blood (1999) [Pubmed]
  30. Correlation of the 4-5 S form and the 8 S form of the cytosolic androgen receptor in murine skeletal muscle. Haase, A., Ofenloch, B., Eisele, K. Biochem. Int. (1983) [Pubmed]
  31. Differential chemokine gene expression in corneal transplant rejection. Yamagami, S., Miyazaki, D., Ono, S.J., Dana, M.R. Invest. Ophthalmol. Vis. Sci. (1999) [Pubmed]
  32. Differential expression of the normal and mutated K-ras alleles in chemically induced thymic lymphomas. Corominas, M., Perucho, M., Newcomb, E.W., Pellicer, A. Cancer Res. (1991) [Pubmed]
  33. The proximal promoter of the aldolase A gene remains active during myogenesis in vitro and muscle development in vivo. Colbert, M.C., Ciejek-Baez, E. Dev. Biol. (1992) [Pubmed]
  34. Pancreatic-type ribonuclease 1 gene duplications in rat species. Dubois, J.Y., Jekel, P.A., Mulder, P.P., Bussink, A.P., Catzeflis, F.M., Carsana, A., Beintema, J.J. J. Mol. Evol. (2002) [Pubmed]
  35. The ribonuclease A superfamily of mammals and birds: identifying new members and tracing evolutionary histories. Cho, S., Beintema, J.J., Zhang, J. Genomics (2005) [Pubmed]
  36. Characterization of novel Pur alpha-binding proteins in mouse brain. Zeng, L.H., Fujimoto, T., Kumamaru, E., Irie, Y., Miki, N., Kuo, C.H. Neurochem. Int. (2004) [Pubmed]
  37. Targeted disruption of the pemphigus vulgaris antigen (desmoglein 3) gene in mice causes loss of keratinocyte cell adhesion with a phenotype similar to pemphigus vulgaris. Koch, P.J., Mahoney, M.G., Ishikawa, H., Pulkkinen, L., Uitto, J., Shultz, L., Murphy, G.F., Whitaker-Menezes, D., Stanley, J.R. J. Cell Biol. (1997) [Pubmed]
  38. Synthesis of germ-line gamma 1 immunoglobulin heavy-chain transcripts in resting B cells: induction by interleukin 4 and inhibition by interferon gamma. Berton, M.T., Uhr, J.W., Vitetta, E.S. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  39. Hypomyelination alters K+ channel expression in mouse mutants shiverer and Trembler. Wang, H., Allen, M.L., Grigg, J.J., Noebels, J.L., Tempel, B.L. Neuron (1995) [Pubmed]
  40. LFA-1 is sufficient in mediating neutrophil emigration in Mac-1-deficient mice. Lu, H., Smith, C.W., Perrard, J., Bullard, D., Tang, L., Shappell, S.B., Entman, M.L., Beaudet, A.L., Ballantyne, C.M. J. Clin. Invest. (1997) [Pubmed]
  41. Multiple c-myb transcript cap sites are variously utilized in cells of mouse haemopoietic origin. Watson, R.J., Dyson, P.J., McMahon, J. EMBO J. (1987) [Pubmed]
  42. Generation of fibrosarcomas in vivo by a retrovirus that expresses the normal B chain of platelet-derived growth factor and mimics the alternative splice pattern of the v-sis oncogene. Pech, M., Gazit, A., Arnstein, P., Aaronson, S.A. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  43. Expression of a transfected H-2Kb gene in B16 cells correlates with suppression of liver metastases in triple immunodeficient mice. Calorini, L., Marozzi, A., Byers, H.R., Waneck, G.L., Lee, K.W., Isselbacher, K.J., Gattoni-Celli, S. Cancer Res. (1992) [Pubmed]
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