Disease relevance of ZRF1

• A putative oncogenic role for MPP11 in head and neck squamous cell cancer [1].
• Serological responses to MPP11 were observed in 7/19 (37%) of patients with acute myeloid leukemia (AML) and 6/16 (38%) of patients with chronic myeloid leukemia (CML), but not in healthy volunteers (0/20) [2].
• IgG antibodies directed against MPP11 were also detected in 25-50% of the sera of patients with solid tumors such as melanoma, renal cell, ovarian and breast carcinoma. mRNA expression of MPP11 was detected in 20/20 AML patients and 7/10 patients with CML [2].

Psychiatry related information on ZRF1

• Its N-terminus has homology to Z-DNA binding protein, Zuotin, that contains DnaJ motif, considered to interact with Hsp70s, and Id binding domain [3].

High impact information on ZRF1

• Unlike Zuo1, a 60S subunit-associated J-protein that is a component of the chaperone machinery that binds nascent polypeptide chains upon their exit from the ribosome, Jjj1 plays a role in ribosome biogenesis [4].
• Overexpression of Jjj1, which is normally approximately 40-fold less abundant than Zuo1, can partially rescue the phenotypes of cells lacking Zuo1 as well as cells lacking Ssb [4].
• Here, we report that MPP11 is localized to the cytosol and associates with ribosomes [5].
• Members of this family, like human MPP11 and mouse MIDA1, have been implicated in the regulation of cell growth [5].
• Thirty-eight percent of tumors examined displayed LOH/AI involving the MPP11 genomic locus [1].

Biological context of ZRF1

• 1. We then analyzed primary head and neck squamous cell tumors (HNSCCs) for loss of heterozygosity/allelic imbalance (LOH/AI) at the MPP11 genomic locus [1].
• Fluorescence in situ hybridization analysis done on the cell lines identified increased chromosome 7 copy number with a concomitant increase in MPP11 copy number [1].
• By real-time PCR, we detected upregulation of MPP11 in leukemic blasts [2].

Anatomical context of ZRF1

• However, immunohistochemical staining of primary tumor sections and Western blot analysis of HNSCC cell lines revealed a tumor-specific high level of expression of MPP11p [1].
• In normal tissues, strong mRNA expression of MPP11 was only detected in testis [2].

Associations of ZRF1 with chemical compounds

• Mutations of L23 reduced NAC ribosome binding in vivo and in vitro, whereas other eukaryotic ribosome-associated factors such as the Hsp70/40 chaperones Ssb or Zuotin were unaffected [6].

References