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Gene Review

GK3P  -  glycerol kinase 3 pseudogene

Homo sapiens

Synonyms: ATP:glycerol 3-phosphotransferase 3, GK 3, GKP3, GKTB, Glycerokinase 3, ...
 
 
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Disease relevance of GK3P

  • Glycerokinase from Cellulomonas sp. was used to develop biosensor based on flow calorimetry for quantitative analysis of glycerol during bioconversion process [1].
 

High impact information on GK3P

  • This labeled ATP was used as a thiophosphoryl group donor in the reactions catalyzed by glycerol kinase (ATP:glycerol 3-phosphotransferase; EC 2.7.1.30) and by hexokinase (ATP:D-hexose 6-phosphotransferase; EC 2.7.1.1) [2].
  • Niddm1i is a major glucose-controlling 16-Mb region in the diabetic GK rat that causes defective insulin secretion and corresponds to loci in humans and mice associated with type 2 diabetes [3].
  • By contrast, a strain carrying the GK genotype proximally in Niddm1i exhibited both intact insulin release in response to high potassium and intact single-cell exocytosis, but insulin secretion was suppressed when stimulated by glucose [3].
  • Congenic strains carrying the GK genotype distally in Niddm1i displayed reduced insulin secretion in response to both glucose and high potassium, as well as decreased single-cell exocytosis [3].
  • The rate of glycerol-dependent ATP-consumption in a system consisting of glycerokinase, ATP, luciferin, and luciferase was determined kinetically as a decrease of the ATP-induced luminescence and used for calculation of the concentration of glycerol [4].
 

Biological context of GK3P

 

Associations of GK3P with chemical compounds

  • Glycerol 3-phosphate (G3P) is produced by glycerol kinase (GK) and is found in several biochemical pathways in different cellular compartments, such as the glycerol phosphate shuttle and glycerophospholipid synthesis [5].
  • The new gamma-phosphate is hydrolyzed to Pi by using glycerokinase and D-glyceraldehyde [7].
  • Effective cGMP-dependence was tested in rings pretreated with soluble guanylate cyclase inhibitors {either 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) or LY-83583} or G kinase inhibitor (KT-5823), and a thiol reductant [dithiothreitol (DTT)] was used to test reversibility of S-nitrosylation [8].
  • Results are compared with determinations made by the solution method that uses the three enzymes glycerokinase, pyruvate kinase and lactate dehydrogenase [9].
 

Analytical, diagnostic and therapeutic context of GK3P

  • Using a combination of sequence analysis and evolutionary comparisons of orthologs between species, we characterized functional domains in the protein required for GK activity [6].

References

  1. Development of enzyme flow calorimeter system for monitoring of microbial glycerol conversion. Stefuca, V., Vostiar, I., Sefcovicov??, J., Katrl??k, J., Mastihuba, V., Greifov??, M., Gemeiner, P. Appl. Microbiol. Biotechnol. (2006) [Pubmed]
  2. Adenosine 5'-O-([gamma-18O]gamma-thio)triphosphate chiral at the gamma-phosphorus: stereochemical consequences of reactions catalyzed by pyruvate kinase, glycerol kinase, and hexokinase. Orr, G.A., Simon, J., Jones, S.R., Chin, G.J., Knowles, J.R. Proc. Natl. Acad. Sci. U.S.A. (1978) [Pubmed]
  3. Separately Inherited Defects in Insulin Exocytosis and {beta}-Cell Glucose Metabolism Contribute to Type 2 Diabetes. Granhall, C., Rosengren, A.H., Renstr??m, E., Luthman, H. Diabetes (2006) [Pubmed]
  4. Sensitive kinetic bioluminescent assay of glycerol release from human fat cells. Björkhem, I., Arner, P., Thore, A., Ostman, J. J. Lipid Res. (1981) [Pubmed]
  5. Cloning and characterization of a putative human glycerol 3-phosphate permease gene (SLC37A1 or G3PP) on 21q22.3: mutation analysis in two candidate phenotypes, DFNB10 and a glycerol kinase deficiency. Bartoloni, L., Wattenhofer, M., Kudoh, J., Berry, A., Shibuya, K., Kawasaki, K., Wang, J., Asakawa, S., Talior, I., Bonne-Tamir, B., Rossier, C., Michaud, J., McCabe, E.R., Minoshima, S., Shimizu, N., Scott, H.S., Antonarakis, S.E. Genomics (2000) [Pubmed]
  6. Conserved family of glycerol kinase loci in Drosophila melanogaster. Martinez Agosto, J.A., McCabe, E.R. Mol. Genet. Metab. (2006) [Pubmed]
  7. A method for determining the positional isotope exchange in a nucleoside triphosphate: cyclization of nucleoside triphosphate by dicyclohexylcarbodiimide. Webb, M.R. Biochemistry (1980) [Pubmed]
  8. S-nitrosoglutathione inhibits alpha1-adrenergic receptor-mediated vasoconstriction and ligand binding in pulmonary artery. Nozik-Grayck, E., Whalen, E.J., Stamler, J.S., McMahon, T.J., Chitano, P., Piantadosi, C.A. Am. J. Physiol. Lung Cell Mol. Physiol. (2006) [Pubmed]
  9. The use of an immobilized glycerol dehydrogenase nylon-tube reactor in the determination of glycerol. Hinsch, W., Sundaram, P.V. Clin. Chim. Acta (1980) [Pubmed]
 
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