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Gene Review

ANXA4  -  annexin A4

Bos taurus

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Disease relevance of ANXA4


High impact information on ANXA4

  • Partial amino acid sequences of p33/41 are highly homologous to those of calcium/phospholipid-binding annexin protein, annexin IV (endonexin), p33/41 exhibited similar calcium/phospholipid-binding activity (Kojima, K., Ogawa, H., Seno, N., Yamamoto, K., Irimura, T., Osawa, T., and Matsumoto, I. (1993) J. Biol. Chem. 267, 20536-20539) [2].
  • The deduced amino acid sequence was identical to that of bovine annexin IV except for one amino acid substitution [2].
  • Phosphorylation mutants elucidate the mechanism of annexin IV-mediated membrane aggregation [3].
  • The propensity of several annexins to generate similar trimeric arrays supports the hypothesis that trimeric complexes of such annexins, including annexin IV, may represent the functional units that interact with membranes [4].
  • SP-A bound to annexin IV under conditions where contaminated lipid was removed [5].

Biological context of ANXA4


Anatomical context of ANXA4


Associations of ANXA4 with chemical compounds

  • Site-directed mutagenesis was used to change the conserved acidic residues (D/E) in these sequences to alanine residues in each of the four domains of bovine annexin IV, singly or in combinations [6].
  • Site-directed mutants that either mimic (Thr6Asp, T6D) or prevent (Thr6Ala, T6A) phosphorylation of threonine 6 were produced for these studies and compared with wild-type annexin IV [3].
  • These fluorimetric assays offer expeditious alternatives to the existing radiolabeled thyrotropin-releasing hormone assays for the determination of PAPII activity [10].
  • The isolated proteins (PAP I-a, PAP I-b and PAP II) were homogeneous in various methods including 7.5% and 20% gel electrophoresis or gel chromatography, and share, in the extract, 85% of the total of the acidic proteins that migrate with the bromophenol blue marker in 7.5% gels [11].

Other interactions of ANXA4

  • However, the augmentation of aggregation of the lung lamellar bodies by annexin IV was attenuated when the lamellar bodies were preincubated with polyclonal anti-SP-A antibodies [5].

Analytical, diagnostic and therapeutic context of ANXA4


  1. Comparison of the expression of native and mutant bovine annexin IV in Escherichia coli using four different expression systems. Nelson, M.R., Creutz, C.E. Protein Expr. Purif. (1995) [Pubmed]
  2. Characterization of carbohydrate-binding protein p33/41: relation with annexin IV, molecular basis of the doublet forms (p33 and p41), and modulation of the carbohydrate binding activity by phospholipids. Kojima, K., Yamamoto, K., Irimura, T., Osawa, T., Ogawa, H., Matsumoto, I. J. Biol. Chem. (1996) [Pubmed]
  3. Phosphorylation mutants elucidate the mechanism of annexin IV-mediated membrane aggregation. Kaetzel, M.A., Mo, Y.D., Mealy, T.R., Campos, B., Bergsma-Schutter, W., Brisson, A., Dedman, J.R., Seaton, B.A. Biochemistry (2001) [Pubmed]
  4. Structure of the trigonal crystal form of bovine annexin IV. Zanotti, G., Malpeli, G., Gliubich, F., Folli, C., Stoppini, M., Olivi, L., Savoia, A., Berni, R. Biochem. J. (1998) [Pubmed]
  5. Ca(2+)-dependent binding of annexin IV to surfactant protein A and lamellar bodies in alveolar type II cells. Sohma, H., Matsushima, N., Watanabe, T., Hattori, A., Kuroki, Y., Akino, T. Biochem. J. (1995) [Pubmed]
  6. Combinatorial mutagenesis of the four domains of annexin IV: effects on chromaffin granule binding and aggregating activities. Nelson, M.R., Creutz, C.E. Biochemistry (1995) [Pubmed]
  7. A study of a highly specific pyroglutamyl aminopeptidase type-II from the membrane fraction of bovine brain. Gallagher, S.P., O'Connor, B. Int. J. Biochem. Cell Biol. (1998) [Pubmed]
  8. Detection of annexin IV in bovine retinal rods. Zernii, E.Y., Tikhomirova, N.K., Philippov, P.P., Senin, I.I. Biochemistry Mosc. (2003) [Pubmed]
  9. Highly polarized expression of carbohydrate-binding protein p33/41 (annexin IV) on the apical plasma membrane of epithelial cells in renal proximal tubules. Kojima, K., Utsumi, H., Ogawa, H., Matsumoto, I. FEBS Lett. (1994) [Pubmed]
  10. The development of two fluorimetric assays for the determination of pyroglutamyl aminopeptidase type-II activity. Gallagher, S.P., O'Leary, R.M., O'Connor, B. Anal. Biochem. (1997) [Pubmed]
  11. Reinvestigation of extremely acidic proteins in bovine brain. Isobe, T., Nakajima, T., Okuyama, T. Biochim. Biophys. Acta (1977) [Pubmed]
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