High impact information on NCAM1

• We found that three out of these five types of MVE express the neural cell adhesion molecule (NCAM) [1].
• These results reveal for the first time that NCAM can be expressed by cultured MVE and may serve in mediating endothelial cell contacts [1].
• Cultured microvascular endothelial cells derived from the bovine corpus luteum possess NCAM-140 [1].
• Since luteal cells also express NCAM-140, this adhesion molecule could in addition be involved in the interactions of luteal cells with MVE [1].
• Peptide signals are observed following transport through the NCAM, even when the peptide is dissolved in solutions containing up to 20% seawater [2].

Anatomical context of NCAM1

• The presence of neural cell adhesion molecule (NCAM) was examined in the neural retina, interphotoreceptor matrix (IPM), and retinal pigment epithelium (RPE) of adult bovine and frog eyes [3].
• Neural cell adhesion molecule (NCAM) in adult vertebrate retinas: tissue localization and evidence against its role in retina-pigment epithelium adhesion [3].
• Differences between the phenotypes related to the structure of the cytoskeleton, the fibronectin matrix and to the expression of cell-cell adhesion molecules from the cadherin family and the NCAM (neuronal cell adhesion molecule) group [4].

Analytical, diagnostic and therapeutic context of NCAM1

• As shown by immunocytochemistry, weak NCAM immunoreactivity occurred mainly in the perinuclear area of cell type 1 [1].
• Western blot analyses substantiated these results: While cell type 1 showed a weak immunoreactive band, cell types 2 and 5 displayed strong NCAM-immunoreactive bands of a molecular weight of approximately 140 kDa (NCAM-140), which was absent in cell types 3 and 4 [1].
• The unique multilayer design allows both the analyte and the spray solutions to reside on the device simultaneously in discrete microfluidic environments that are spatially separated by a polycarbonate track-etched, nanocapillary array membrane (NCAM) [2].

References