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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
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Disease relevance of Microfluidics


High impact information on Microfluidics

  • Microfluidic networks (microFNs) were used to pattern biomolecules with high resolution on a variety of substrates (gold, glass, or polystyrene) [4].
  • This Account summarizes techniques for fabrication and applications in biomedicine of microfluidic devices fabricated in poly(dimethylsiloxane) (PDMS) [5].
  • To improve visualization and the control of cell position, we have developed a simple alternative patch clamp technique based on microfluidic junctions between a main chamber and lateral recording capillaries, all fabricated by micromolding of polydimethylsiloxane (PDMS) [6].
  • Using electroosmosis to drive flow through microfluidic channels, we pulse minute quantities of a bradykinin solution through four 5-microm apertures onto PC12 cells and show stimulation of individual cells using a Ca(2+)-sensitive fluorescent dye [7].
  • To test these models, we developed a microfluidic device capable of partially stimulating an islet, while allowing observation of the NAD(P)H and [Ca2+]i responses [8].

Biological context of Microfluidics


Anatomical context of Microfluidics

  • Previous reports on stimulating NO production using an immobilized endothelium in microfluidic channels were limited by the requirement of ATP concentrations of at least 100 muM, a value that is not physiologically relevant [14].
  • The electrokinetic focusing and the resultant accelerated electrophoretic motion of polystyrene particles and red blood cells were visualized in microfluidic cross-channels [15].
  • The ability to monitor NO production with ATP concentrations that are similar to in vivo levels of ATP in the microcirculation represents a major advance in the use of microfluidic technology as an in vitro model of the microcirculation [14].
  • Local induction of acetylcholine receptor clustering in myotube cultures using microfluidic application of agrin [16].
  • Based on the large surface area to volume ratio of porous membrane media, adsorbed BSA onto the PVDF membranes enables high resolution separation of racemic mixtures with sample consumption of sub-nanogram or less in the integrated microfluidic networks [17].

Associations of Microfluidics with chemical compounds

  • There is a wide range of literature on soft lithography, organic surface science (especially self-assembled monolayers of organic thiols adsorbed on gold) and microfluidics [18].
  • Applications such as tissue engineering, drug delivery, the fabrication of microfluidic devices and the preparation of high-density cell arrays employ hydrogel materials that are often prepared by this technique [19].
  • The Letter reports an experimental study of microfluidic droplets produced in T junctions and subjected to a local periodic forcing [20].
  • The principle is demonstrated using a microfluidic chip made of poly(methyl methacrylate) with integrated soft ferromagnetic plate structures [21].
  • Linear and rapid separation of proteins was achieved in the polyacrylamide-coated COC microfluidic device [22].

Gene context of Microfluidics

  • The SARS virus species can be analyzed with high positive rate and rapidity on the microfluidic chip system [23].
  • We developed a microfluidic system with microstructured membranes for exposing neutrophils to fast and precise changes between stable, linear gradients of the known chemoattractant Interleukin-8 (IL-8) [24].
  • Human NSCs (hNSCs) from the developing cerebral cortex were cultured for more than 1 week in the microfluidic device while constantly exposed to a continuous gradient of a growth factor (GF) mixture containing epidermal growth factor (EGF), fibroblast growth factor 2 (FGF2) and platelet-derived growth factor (PDGF) [25].
  • The three-dimensional topology of the microfluidic network in the stamp makes this technique a versatile one with which to pattern multiple types of proteins and cells in complex, discontinuous structures on a surface [26].
  • To obtain the single-turnover rate constant (k = 1100 +/- 250 s(-1)), four new features for this microfluidics platform were demonstrated: (i) rapid on-chip dilution, (ii) multiple time range access, (iii) biocompatibility with RNase A, and (iv) explicit treatment of mixing for improving time resolution of the system [27].

Analytical, diagnostic and therapeutic context of Microfluidics


  1. Label-free detection of small-molecule-protein interactions by using nanowire nanosensors. Wang, W.U., Chen, C., Lin, K.H., Fang, Y., Lieber, C.M. Proc. Natl. Acad. Sci. U.S.A. (2005) [Pubmed]
  2. A microfluidic bioreactor based on hydrogel-entrapped E. coli: cell viability, lysis, and intracellular enzyme reactions. Heo, J., Thomas, K.J., Seong, G.H., Crooks, R.M. Anal. Chem. (2003) [Pubmed]
  3. Microfluidic genetic analysis with an integrated a-Si:H detector. Kamei, T., Toriello, N.M., Lagally, E.T., Blazej, R.G., Scherer, J.R., Street, R.A., Mathies, R.A. Biomedical microdevices. (2005) [Pubmed]
  4. Patterned delivery of immunoglobulins to surfaces using microfluidic networks. Delamarche, E., Bernard, A., Schmid, H., Michel, B., Biebuyck, H. Science (1997) [Pubmed]
  5. Poly(dimethylsiloxane) as a material for fabricating microfluidic devices. McDonald, J.C., Whitesides, G.M. Acc. Chem. Res. (2002) [Pubmed]
  6. Mammalian electrophysiology on a microfluidic platform. Ionescu-Zanetti, C., Shaw, R.M., Seo, J., Jan, Y.N., Jan, L.Y., Lee, L.P. Proc. Natl. Acad. Sci. U.S.A. (2005) [Pubmed]
  7. Localized chemical release from an artificial synapse chip. Peterman, M.C., Noolandi, J., Blumenkranz, M.S., Fishman, H.A. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  8. Microfluidic glucose stimulation reveals limited coordination of intracellular Ca2+ activity oscillations in pancreatic islets. Rocheleau, J.V., Walker, G.M., Head, W.S., McGuinness, O.P., Piston, D.W. Proc. Natl. Acad. Sci. U.S.A. (2004) [Pubmed]
  9. Detection of DNA fragmentation in a single apoptotic cardiomyocyte by electrophoresis on a microfluidic device. Klepárník, K., Horký, M. Electrophoresis (2003) [Pubmed]
  10. Single-nucleotide polymorphism analysis by allele-specific extension of fluorescently labeled nucleotides in a microfluidic flow-through device. Russom, A., Ahmadian, A., Andersson, H., Nilsson, P., Stemme, G. Electrophoresis (2003) [Pubmed]
  11. Application of on-chip cell cultures for the detection of allergic response. Matsubara, Y., Murakami, Y., Kobayashi, M., Morita, Y., Tamiya, E. Biosensors & bioelectronics. (2004) [Pubmed]
  12. Kinetics of ultraviolet and plasma surface modification of poly(dimethylsiloxane) probed by sum frequency vibrational spectroscopy. Ye, H., Gu, Z., Gracias, D.H. Langmuir : the ACS journal of surfaces and colloids. (2006) [Pubmed]
  13. Neutrophil migration in opposing chemoattractant gradients using microfluidic chemotaxis devices. Lin, F., Nguyen, C.M., Wang, S.J., Saadi, W., Gross, S.P., Jeon, N.L. Annals of biomedical engineering. (2005) [Pubmed]
  14. Fluorescence Monitoring of ATP-Stimulated, Endothelium-Derived Nitric Oxide Production in Channels of a Poly(dimethylsiloxane)-Based Microfluidic Device. D'Amico Oblak, T., Root, P., Spence, D.M. Anal. Chem. (2006) [Pubmed]
  15. Focused electrophoretic motion and selected electrokinetic dispensing of particles and cells in cross-microchannels. Xuan, X., Li, D. Electrophoresis (2005) [Pubmed]
  16. Local induction of acetylcholine receptor clustering in myotube cultures using microfluidic application of agrin. Tourovskaia, A., Kosar, T.F., Folch, A. Biophys. J. (2006) [Pubmed]
  17. High-resolution chiral separation using microfluidics-based membrane chromatography. Wang, P.C., Gao, J., Lee, C.S. Journal of chromatography. A. (2002) [Pubmed]
  18. Combining microscience and neurobiology. Weibel, D.B., Garstecki, P., Whitesides, G.M. Curr. Opin. Neurobiol. (2005) [Pubmed]
  19. Light-activated hydrogel formation via the triggered folding and self-assembly of a designed peptide. Haines, L.A., Rajagopal, K., Ozbas, B., Salick, D.A., Pochan, D.J., Schneider, J.P. J. Am. Chem. Soc. (2005) [Pubmed]
  20. Arnold tongues in a microfluidic drop emitter. Willaime, H., Barbier, V., Kloul, L., Maine, S., Tabeling, P. Phys. Rev. Lett. (2006) [Pubmed]
  21. Manipulation of self-assembled structures of magnetic beads for microfluidic mixing and assaying. Rida, A., Gijs, M.A. Anal. Chem. (2004) [Pubmed]
  22. Isoelectric focusing in cyclic olefin copolymer microfluidic channels coated by polyacrylamide using a UV photografting method. Li, C., Yang, Y., Craighead, H.G., Lee, K.H. Electrophoresis (2005) [Pubmed]
  23. Determination of SARS-coronavirus by a microfluidic chip system. Zhou, X., Liu, D., Zhong, R., Dai, Z., Wu, D., Wang, H., Du, Y., Xia, Z., Zhang, L., Mei, X., Lin, B. Electrophoresis (2004) [Pubmed]
  24. Microfluidic system for measuring neutrophil migratory responses to fast switches of chemical gradients. Irimia, D., Liu, S.Y., Tharp, W.G., Samadani, A., Toner, M., Poznansky, M.C. Lab on a chip. (2006) [Pubmed]
  25. Human neural stem cell growth and differentiation in a gradient-generating microfluidic device. Chung, B.G., Flanagan, L.A., Rhee, S.W., Schwartz, P.H., Lee, A.P., Monuki, E.S., Jeon, N.L. Lab on a chip. (2005) [Pubmed]
  26. Patterned deposition of cells and proteins onto surfaces by using three-dimensional microfluidic systems. Chiu, D.T., Jeon, N.L., Huang, S., Kane, R.S., Wargo, C.J., Choi, I.S., Ingber, D.E., Whitesides, G.M. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  27. Millisecond kinetics on a microfluidic chip using nanoliters of reagents. Song, H., Ismagilov, R.F. J. Am. Chem. Soc. (2003) [Pubmed]
  28. Isoelectric focusing in a poly(dimethylsiloxane) microfluidic chip. Cui, H., Horiuchi, K., Dutta, P., Ivory, C.F. Anal. Chem. (2005) [Pubmed]
  29. Immobilization of DNA hydrogel plugs in microfluidic channels. Olsen, K.G., Ross, D.J., Tarlov, M.J. Anal. Chem. (2002) [Pubmed]
  30. Microchip capillary electrophoresis with a cellulose-DNA-modified screen-printed electrode for the analysis of neurotransmitters. Johirul, M., Shiddiky, A., Kim, R.E., Shim, Y.B. Electrophoresis (2005) [Pubmed]
  31. Microbioassay system for antiallergic drug screening using suspension cells retaining in a poly(dimethylsiloxane) microfluidic device. Tokuyama, T., Fujii, S., Sato, K., Abo, M., Okubo, A. Anal. Chem. (2005) [Pubmed]
  32. Surface characterization using chemical force microscopy and the flow performance of modified polydimethylsiloxane for microfluidic device applications. Wang, B., Abdulali-Kanji, Z., Dodwell, E., Horton, J.H., Oleschuk, R.D. Electrophoresis (2003) [Pubmed]
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