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Gene Review

FN1  -  fibronectin 1

Bos taurus

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Disease relevance of FN1


High impact information on FN1


Chemical compound and disease context of FN1


Biological context of FN1


Anatomical context of FN1

  • For many pathogens, adherence and/or invasion involve association with host extracellular matrix molecules, such as fibronectin (Fn) [1].
  • Thus follicles and corpora lutea express isoforms of fibronectin usually expressed in developing tissues [16].
  • Bovine ovarian follicles (0.5-9 mm diameter) and corpora lutea (cyclic, early to late mid-luteal phase) were shown to express ED-A+, ED-A-, ED-B+, ED-B-, V+ and V fibronectin isoforms, similar to the liver, lung and kidney of fetuses, but generally not of adult animals [16].
  • Adherence of chondrocytes to fibronectin enhanced proteoglycan synthesis by twofold (vs. albumin) [3].
  • Endothelial cell growth can be controlled in chemically-defined medium by varying the density of FN coated on the substratum (Ingber, D. E., and J. Folkman. J. Cell Biol. 1989. 109:317-330) [18].

Associations of FN1 with chemical compounds


Physical interactions of FN1

  • The C-terminal portion of BSP-30K is organized in a tandem of 40-44-residue domains each sharing the consensus pattern of the gelatin-binding fibronectin type II module [21].
  • We have previously shown that both heparin and fibronectin have two binding sites on the TSP-1 subunit which may require conformational change for their occupancy (R. Dardik and J. Lahav, 1987, Eur. J. Biochem. 168, 347; ibid 1989, 185, 581) [22].
  • The combination of SP and IGF-1 significantly increased the number of cells attached to the fibronectin matrix and the expression of integrin alpha 5 [23].
  • Further, osteoblastic cells on the grooved surfaces also displayed a strong labelling for fibronectin at the cytoplasmic extensions coupled with intense osteonectin expression in comparison to the rough surfaced implants [24].
  • Adhesion to a 60 kd tryptic fragment of fibronectin that is not involved in integrin-mediated cell binding was also increased by TGF-beta [25].

Regulatory relationships of FN1


Other interactions of FN1

  • The presence of endogenously produced active TGF-beta in the culture media was shown to modulate the behavior of the cell cultures as evidenced by the effects of TGF-beta-neutralizing antisera on cell size and fibronectin production [31].
  • The results of distance geometry and restrained molecular dynamics calculations indicate that the global fold of the PDC-109 b domain, a type II module related to those found in fibronectin, is somewhat different from that predicted by modeling the structure on the basis of homology between type II and kringle units [32].
  • Seven days of retinoic acid treatment resulted in decreased attachment to fibronectin, MGP, and collagen [33].
  • The collagen-binding domain of bovine fibronectin (type II sequence) was also found to be highly homologous to both BSP-A3 and PDC-109 [34].
  • Cyclic compression of articular cartilage at 0.1 Hz and higher was consistently associated with a dramatic increase in the synthesis of COMP as well as fibronectin [35].

Analytical, diagnostic and therapeutic context of FN1

  • Southern blot studies suggest that human FN is coded by a unique gene [15].
  • PCR products from bovine fetal liver cDNA were determined to be bovine fibronectin by the correct product size and DNA sequence homology to other species, and to the known bovine amino acid sequence [16].
  • Based upon the timing of appearance of conditioning factors on the substratum and the immunofluorescence patterns, it seems that the diffusely organized fibronectin on the substratum constitutes the sites to which cell attachment occurs [6].
  • Intravenous injection of bovine serum albumin-antibovine serum albumin complexes or colloidal carbon was combined with histological staining for endogenous peroxidase, fibronectin, laminin, or a lectin, Bandeiraea simplicifolia agglutinin I [36].
  • Bovine aortic endothelial cell (BAEC) attachments to laminin, fibronectin, and fibrinogen are inhibited by soluble arginine-glycine-aspartate (RGD)-containing peptides, and YGRGDSP activity is responsive to titration of either soluble peptide or matrix protein [37].


  1. Adherence of Pasteurella multocida to fibronectin. Dabo, S.M., Confer, A.W., Hartson, S.D. Vet. Microbiol. (2005) [Pubmed]
  2. Antibodies to basement membrane collagen and to laminin are present in sera from patients with poststreptococcal glomerulonephritis. Kefalides, N.A., Pegg, M.T., Ohno, N., Poon-King, T., Zabriskie, J., Fillit, H. J. Exp. Med. (1986) [Pubmed]
  3. Outside-in signaling in the chondrocyte. Nitric oxide disrupts fibronectin-induced assembly of a subplasmalemmal actin/rho A/focal adhesion kinase signaling complex. Clancy, R.M., Rediske, J., Tang, X., Nijher, N., Frenkel, S., Philips, M., Abramson, S.B. J. Clin. Invest. (1997) [Pubmed]
  4. Bovine brain and pituitary fibroblast growth factors: comparison of their abilities to support the proliferation of human and bovine vascular endothelial cells. Gospodarowicz, D., Cheng, J., Lirette, M. J. Cell Biol. (1983) [Pubmed]
  5. Glanzmann thrombasthenia: deficient binding of von Willebrand factor to thrombin-stimulated platelets. Ruggeri, Z.M., Bader, R., de Marco, L. Proc. Natl. Acad. Sci. U.S.A. (1982) [Pubmed]
  6. Initial adhesion of human fibroblasts in serum-free medium: possible role of secreted fibronectin. Grinnell, F., Feld, M.K. Cell (1979) [Pubmed]
  7. Heterogeneity of membrane phospholipid mobility in endothelial cells depends on cell substrate. Nakache, M., Schreiber, A.B., Gaub, H., McConnell, H.M. Nature (1985) [Pubmed]
  8. Fibronectin receptors on Trypanosoma cruzi trypomastigotes and their biological function. Ouaissi, M.A., Afchain, D., Capron, A., Grimaud, J.A. Nature (1984) [Pubmed]
  9. An endocrine approach to the control of epidermal growth: serum-free cultivation of human keratinocytes. Maciag, T., Nemore, R.E., Weinstein, R., Gilchrest, B.A. Science (1981) [Pubmed]
  10. Influence of cell shape and surface charge on attachment of Mycoplasma pneumoniae to glass surfaces. Feldner, J., Bredt, W., Kahane, I. J. Bacteriol. (1983) [Pubmed]
  11. Nitric oxide alters chondrocyte function by disrupting cytoskeletal signaling complexes. Clancy, R. Osteoarthr. Cartil. (1999) [Pubmed]
  12. Genetic relatedness and phenotypic characteristics of Treponema associated with human periodontal tissues and ruminant foot disease. Edwards, A.M., Dymock, D., Woodward, M.J., Jenkinson, H.F. Microbiology (Reading, Engl.) (2003) [Pubmed]
  13. Surfactant-immobilized fibronectin enhances bioactivity and regulates sensory neurite outgrowth. Biran, R., Webb, K., Noble, M.D., Tresco, P.A. J. Biomed. Mater. Res. (2001) [Pubmed]
  14. Fibronectin metabolism of cartilage explants in response to the frequency of intermittent loading. Wolf, A., Raiss, R.X., Steinmeyer, J. J. Orthop. Res. (2003) [Pubmed]
  15. Isolation and characterization of cDNA clones for human and bovine fibronectins. Kornblihtt, A.R., Vibe-Pedersen, K., Baralle, F.E. Proc. Natl. Acad. Sci. U.S.A. (1983) [Pubmed]
  16. Characterization of the expression of the alternative splicing of the ED-A, ED-B and V regions of fibronectin mRNA in bovine ovarian follicles and corpora lutea. De Candia, L.M., Rodgers, R.J. Reprod. Fertil. Dev. (1999) [Pubmed]
  17. Subendothelial retention of lipoprotein (a). Evidence that reduced heparan sulfate promotes lipoprotein binding to subendothelial matrix. Pillarisetti, S., Paka, L., Obunike, J.C., Berglund, L., Goldberg, I.J. J. Clin. Invest. (1997) [Pubmed]
  18. Control of intracellular pH and growth by fibronectin in capillary endothelial cells. Ingber, D.E., Prusty, D., Frangioni, J.V., Cragoe, E.J., Lechene, C., Schwartz, M.A. J. Cell Biol. (1990) [Pubmed]
  19. Complete primary structure of bovine plasma fibronectin. Skorstengaard, K., Jensen, M.S., Sahl, P., Petersen, T.E., Magnusson, S. Eur. J. Biochem. (1986) [Pubmed]
  20. Binding and covalent cross-linking of purified von Willebrand factor to native monomeric collagen. Bockenstedt, P., McDonagh, J., Handin, R.I. J. Clin. Invest. (1986) [Pubmed]
  21. The primary structure of BSP-30K, a major lipid-, gelatin-, and heparin-binding glycoprotein of bovine seminal plasma. Calvete, J.J., Mann, K., Sanz, L., Raida, M., Töpfer-Petersen, E. FEBS Lett. (1996) [Pubmed]
  22. Functional changes in the conformation of thrombospondin-1 during complexation with fibronectin or heparin. Dardik, R., Lahav, J. Exp. Cell Res. (1999) [Pubmed]
  23. Up-regulation of integrin alpha 5 expression by combination of substance P and insulin-like growth factor-1 in rabbit corneal epithelial cells. Nakamura, M., Chikama, T., Nishida, T. Biochem. Biophys. Res. Commun. (1998) [Pubmed]
  24. Influence of titanium surfaces on attachment of osteoblast-like cells in vitro. Jayaraman, M., Meyer, U., Bühner, M., Joos, U., Wiesmann, H.P. Biomaterials (2004) [Pubmed]
  25. Transforming growth factor-beta increases adhesion but not migration of bovine bronchial epithelial cells to matrix proteins. Spurzem, J.R., Sacco, O., Rickard, K.A., Rennard, S.I. J. Lab. Clin. Med. (1993) [Pubmed]
  26. Fibronectin fragments upregulate insulin-like growth factor binding proteins in chondrocytes. Purple, C.R., Untermann, T.G., Pichika, R., Homandberg, G.A. Osteoarthr. Cartil. (2002) [Pubmed]
  27. Extracellular matrix production regulation by TGF-beta in corneal endothelial cells. Usui, T., Takase, M., Kaji, Y., Suzuki, K., Ishida, K., Tsuru, T., Miyata, K., Kawabata, M., Yamashita, H. Invest. Ophthalmol. Vis. Sci. (1998) [Pubmed]
  28. Electric field-directed cell motility involves up-regulated expression and asymmetric redistribution of the epidermal growth factor receptors and is enhanced by fibronectin and laminin. Zhao, M., Dick, A., Forrester, J.V., McCaig, C.D. Mol. Biol. Cell (1999) [Pubmed]
  29. Evaluation of extracellular matrix proteins and tissue inhibitor of matrix metalloproteinases-2 on bovine inner cell mass outgrowth in vitro. Schilperoort-Haun, K.R., Menino, A.R. In Vitro Cell. Dev. Biol. Anim. (2002) [Pubmed]
  30. Surface adhesion and attachment factors in bone morphogenetic protein-induced chondrogenesis in vitro. Katoh, R., Urist, M.R. Clin. Orthop. Relat. Res. (1993) [Pubmed]
  31. Spontaneous production of transforming growth factor-beta 2 by primary cultures of bronchial epithelial cells. Effects on cell behavior in vitro. Sacco, O., Romberger, D., Rizzino, A., Beckmann, J.D., Rennard, S.I., Spurzem, J.R. J. Clin. Invest. (1992) [Pubmed]
  32. Sequence-specific 1H NMR assignments and structural characterization of bovine seminal fluid protein PDC-109 domain b. Constantine, K.L., Ramesh, V., Bányai, L., Trexler, M., Patthy, L., Llinás, M. Biochemistry (1991) [Pubmed]
  33. Modulation of integrin-mediated attachment of chondrocytes to extracellular matrix proteins by cations, retinoic acid, and transforming growth factor beta. Loeser, R.F. Exp. Cell Res. (1994) [Pubmed]
  34. Complete amino acid sequence of BSP-A3 from bovine seminal plasma. Homology to PDC-109 and to the collagen-binding domain of fibronectin. Seidah, N.G., Manjunath, P., Rochemont, J., Sairam, M.R., Chrétien, M. Biochem. J. (1987) [Pubmed]
  35. Cyclic compression of articular cartilage explants is associated with progressive consolidation and altered expression pattern of extracellular matrix proteins. Wong, M., Siegrist, M., Cao, X. Matrix Biol. (1999) [Pubmed]
  36. Defect of sinusoidal Fc receptors and immune complex uptake in CCl4-induced liver cirrhosis in rats. Muro, H., Shirasawa, H., Kosugi, I., Ito, I. Gastroenterology (1990) [Pubmed]
  37. Spatiotemporal segregation of endothelial cell integrin and nonintegrin extracellular matrix-binding proteins during adhesion events. Basson, C.T., Knowles, W.J., Bell, L., Albelda, S.M., Castronovo, V., Liotta, L.A., Madri, J.A. J. Cell Biol. (1990) [Pubmed]
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