Disease relevance of TACR2

• The gene encoding the nonstructural protein (NSP) of O/SKR/2000 foot-and-mouth disease virus (FMDV) was constructed to express under the polyhedron promoter of baculovirus [1].
• Comparison between FMDV O/SKR/2000 and other strains indicates that overall the number of amino acids appears to be conserved without any deletion in either NSP or capsid proteins, thus, suggesting that O/SKR/2000 evolved with minor difference from preexisting strains [2].
• Although NK-2 receptor-mediated IP3 formation involves activation of a pertussis toxin-insensitive G-protein, increased cAMP production is largely a secondary response and can be at least partially attributed to autocrine stimulation by endogenously generated eicosanoids, particularly PGE2 [3].

High impact information on TACR2

• Characterization of a tachykinin peptide NK2 receptor transfected into murine fibroblast B82 cells [4].
• In comparison with membranes from the hamster urinary bladder, a tissue enriched in NK2 receptors, NK2 receptor antagonists displayed markedly different potencies, either more or less potent, in inhibiting specific binding in membranes of the transfected cells [4].
• Similar observations of NKA-induced IP3 production, Ca2+ mobilization, arachidonic acid liberation, and cAMP formation have been made previously following expression of the bovine NK-2 receptor in Chinese hamster ovary (CHO) epithelial cells [5].
• While NK2 receptor antagonists had no effect on inositol monophosphate accumulation, substance P and senktide both produced a small degree of desensitization [6].
• The deduced amino acid sequences of the structural and nonstructural proteins (NSP) of the O/SKR/2000 virus were analyzed for the sequence similarity among type O strains [2].

Biological context of TACR2

• Molecular cloning and sequencing of the genome of foot-and-mouth disease virus (FMDV) O/SKR/2000, one of PanAsia strain, were performed from FMDV infected cattle [2].
• We investigated some of the second messenger and cellular processes under control by the recombinant bovine NK-2 receptor stably expressed in Chinese hamster ovary cells [3].
• The overall nucleotide sequence divergence of the VP1 region among the 4 isolates in 2002 was 0 to 1.4%, but between O/SKR/2002 and O/SKR/2000 isolates was 1.9-4.9% [7].

Associations of TACR2 with chemical compounds

• After chronic exposure to neurokinin A, a time-dependent and recoverable desensitization of inositol monophosphate accumulation occurred in B82 fibroblasts transfected with cDNAs encoding for the bovine stomach NK2 receptor [6].

Analytical, diagnostic and therapeutic context of TACR2

• Also, animal experiments indicated that O/SKR/2000 is not host-restricted and develop the clinical signs in the main susceptible livestock species (cattle and pigs) [8].

References