Disease relevance of Igf2bp1

• ZBP1 reexpression also inhibited metastasis from tumors [1].

High impact information on Igf2bp1

• Reexpression of ZBP1 in metastatic cells with otherwise low levels of ZBP1 reestablished normal patterns of beta-actin mRNA targeting and suppressed chemotaxis and invasion in primary tumors [1].
• These cis-acting elements bind the trans-acting factors fibroblast ZBP-1 and hnRNP A2, respectively [2].
• Here, we show that ZBP1 is required for the localization of beta-actin mRNA to dendrites [3].
• Previous studies have shown that beta-actin mRNA localization to the leading edge of fibroblasts or the growth cones of developing neurites involved a specific interaction between a zipcode sequence in the 3' untranslated region and the mRNA-binding protein zipcode-binding protein-1 (ZBP1) [3].
• The activity-dependent trafficking of ZBP1 and its dynamic movements within dendritic spines provide new evidence to implicate RNA binding proteins as regulators of mRNA transport to activated synapses in response to synaptic activity [4].

Biological context of Igf2bp1

• Transfection of an EGFP construct, also with the zipcode, resulted in recruitment of endogenous ZBP1 and beta-actin mRNA into dendrites and similarly increased the density of dendritic filopodia [3].
• We used fluorescence microscopy and digital imaging techniques applied to both fixed and live cultured hippocampal neurons to visualize the localization of the mRNA binding protein, zipcode binding protein 1 (ZBP1), and its dynamic movements in response to KCl-induced depolarization at high spatial and temporal resolution [4].
• Partial amino acid sequences of ZBPP-1 showed identity with rat xanthine dehydrogenase (XD)/xanthine oxidase (XO) [5].

Anatomical context of Igf2bp1

• Thus, ZBPP-1 is an 85 kDa fragment of XD/XO in Paneth cells [5].

Co-localisations of Igf2bp1

• A population of ZBP1 granules colocalized with beta-actin mRNA, and their spatial association in dendrites was increased by KCl depolarization [4].

Analytical, diagnostic and therapeutic context of Igf2bp1

• With the use of immunofluorescence, image deconvolution, and three-dimensional reconstruction, ZBP1 was localized in the form of granules that were distributed in dendrites, spines, and subsynaptic sites [4].
• Live cell imaging of single cells before and after perfusion of KCl revealed the rapid and directed efflux of ZBP1 granules from the cell body into dendrites in a proximo-distal gradient [4].

References