Disease relevance of Myo31DF

• Here we identify the conserved type ID unconventional myosin 31DF gene (Myo31DF) as a unique situs inversus locus in Drosophila [1].

Psychiatry related information on Myo31DF

• Thus Myo31DF is a dextral gene with actin-based motor activity controlling situs choice [1].

High impact information on Myo31DF

• In the adult gut, MIA and MIB both exhibit a microvillar component as MIA attains a more apical position in addition to its previous terminal web locale [2].
• Characterization of myosin-IA and myosin-IB, two unconventional myosins associated with the Drosophila brush border cytoskeleton [2].
• MIA and MIB, both found predominantly at the basolateral domain of immature enterocytes, exhibit increased expression at the apical domain of differentiated enterocytes co-incident with microvillus assembly [2].
• Characterization of clones from a Drosophila Class IA- cDNA library reveals that 18S rRNA and transcripts derived from intermediate repeat DNA constitute the bulk of such molecules [3].
• Disruption of actin filaments in the hindgut epithelium randomizes the handedness of the embryonic gut, suggesting that Myo31DF function requires the actin cytoskeleton [4].

Anatomical context of Myo31DF

• Consistent with this, we find that Myo31DF colocalizes with the cytoskeleton [4].
• We find that Myo31DF is required in the hindgut epithelium for normal embryonic handedness [4].
• Myo31DF mutations reverse the dextral looping of genitalia, a prominent left-right marker in adult flies [1].
• Here we find that the handedness of the embryonic gut and the adult gut and testes is reversed (not randomized) in viable and fertile homozygous Myo31DF mutants [4].
• Like mouse inversin, Myo31DF interacts and colocalizes with beta-catenin, suggesting that situs inversus genes can direct left-right development through the adherens junction [1].

Other interactions of Myo31DF

• Although both are myosins-I from fly, myosin-IA at cytological locus 31D-F and myosin-IB at cytological locus 61F appear to be more similar to their vertebrate homologs than they are to each other [5].

Analytical, diagnostic and therapeutic context of Myo31DF

• Just as the sequence comparisons demonstrate a disparity between myosin-IA and myosin-IB, Northern blot analysis of these two unconventional myosins indicates distinct patterns of temporal expression [5].
• Colocalization of MIA and MIB to larval and adult gut by confocal microscopy demonstrates distinct but overlapping subcellular distributions of these two proteins [2].
• The molecular cloning and characterization of Drosophila melanogaster myosin-IA and myosin-IB [5].
• Primary sequence analyses of both the head and tail domains of the known class I myosins illustrate a division of the metazoan myosin-I family into four distinct subclasses with myosin-IA and myosin-IB as members of two of these groups [5].

References