High impact information on AQP6

• Closely spaced tandem arrangement of AQP2, AQP5, and AQP6 genes in a 27-kilobase segment at chromosome locus 12q13 [1].
• PCR analysis of a human/rodent somatic hybrid panel localized the hKID gene to chromosome 12 [2].
• Expression of hKID cRNA in Xenopus oocytes did not increase glycerol or urea permeability, but increased osmotic water permeability from (2.8 +/- 0.5) x 10(-4) to (7.4 +/- 0.7) x 10(-4) cm/s (10 degrees C) in a mercurial-sensitive manner [2].
• Sequence comparison of hKID cDNA with a cloned 21-kb genomic DNA indicated three introns (lengths 0.7, 0.25, and 0.4 kb) separating four exons with boundaries at amino acids 121, 174, and 201 [2].
• A 1.8-kb cDNA clone (designed hKID, gene symbol AQP2L) with homology to the aquaporins was isolated from a human kidney cDNA library [2].

Biological context of AQP6

• The intracellular distribution indicates that AQP6 has a function distinct from trans-epithelial water movement [3].

Anatomical context of AQP6

• AQP6 is localized intracellular vesicles in the kidney collecting duct cells [4].
• Presence or absence at the plasma membrane of AQP6 constructs was confirmed by electrophysiological methods [3].
• BACKGROUND INFORMATION: Most AQPs (aquaporins) function at the plasma membrane, however AQP6 is exclusively localized to membranes of intracellular vesicles in acid-secreting type-A intercalated cells of renal collecting ducts [3].

Associations of AQP6 with chemical compounds

• The passive water permeability ( L(p)) of AQP6 is activated by Hg(2+) [5].
• The L(p) of AQP6 was determined from brief additions or removals of mannitol from the bathing solution, and compared to the L(p) obtained from adding glycerol or urea [5].

Analytical, diagnostic and therapeutic context of AQP6

• Genomic Southern blot indicated a single-copy hKID gene [2].
• Chromosomal fluorescence in situ hybridization mapped the hKID (AQP2L) gene to chromosome locus 12q13, the same location as the AQP [2].
• RESULTS: We show by mutational analyses and immunofluorescence that the N-terminus of AQP6 is a determinant for its intracellular localization [3].

References