High impact information on Eip71CD

• We have identified ecdysone-response elements (EcREs) by studying regulation of the steroid-responsive Drosophila Eip28/29 gene [1].
• Three short receptor-binding regions near Eip28/29 have been identified: Prox and Dist [521 and 2295 nucleotides, respectively, downstream of the poly(A) site] are probably required for the Eip28/29 response in cell lines; Upstream (-440) is unnecessary for that response [1].
• Identification of ecdysone response elements by analysis of the Drosophila Eip28/29 gene [1].
• Consistent with these computational screens, growth of Saccharomyces cerevisiae SelR and MsrA mutant strains was inhibited, and the strain lacking both genes could not grow, in the presence of H2O2 and methionine sulfoxide [2].
• Alternative splicing generates two distinct Eip28/29 gene transcripts in Drosophila Kc cells [3].

Biological context of Eip71CD

• By comparisons with complementary DNA sequences and by nuclease protection experiments we have derived a complete structure for the Eip28/29 transcription unit [4].
• Ecdysone induction of MsrA protects against oxidative stress in Drosophila [5].
• An investigation of Eip28/29 gene expression in intact animals has led to the discovery of two new genes on its 5' flank [6].
• Diverse expression of overlapping genes: the Drosophila Eip28/29 gene and its upstream neighbors [6].
• In each mode, the longer transcript results from use of a polyadenylation site within the 5' exon of Eip28/29 [6].

Anatomical context of Eip71CD

• In mammals, a single MsrA gene is known, however, its product is present in cytosol, nucleus, and mitochondria [7].
• Most striking are the induction of Eip28/29 transcripts in numerous tissues at the last larval molt, their induction in the epidermis at the time of the 'late 3rd transition', their extinction in the same tissue by the premetamorphic ecdysone peak, and their induction by that peak in the lymph gland, hemocytes and proventriculus [8].

Associations of Eip71CD with chemical compounds

• A Drosophila MsrA gene was also cloned, and the recombinant enzyme was found to be metal-free and specific for methionine S-sulfoxide and to employ a similar sulfenic acid/disulfide mechanism [9].

Regulatory relationships of Eip71CD

• The Drosophila mex1 gene is one of several genes clustered within a 10-kb interval of polytene region 71CD that includes the ecdysone-regulated Eip28/29 gene. mex1 is expressed in several developmental stages, with gene transcripts accumulating initially in 9- to 12-hr embryos [10].

Other interactions of Eip71CD

• The methionine sulfoxide reductases MsrA and MsrB reduce Met(O) to Met in epimer-specific fashion [5].
• Structure of the Eip28/29 gene, an ecdysone-inducible gene from Drosophila [4].
• Tissue-specific ecdysone responses: regulation of the Drosophila genes Eip28/29 and Eip40 during larval development [8].

References