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Gene Review

gutB  -  sorbitol dehydrogenase

Escherichia coli UTI89

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Disease relevance of gutB


High impact information on gutB

  • 0. A comparison of the kinetic data of MDH and mammalian sorbitol dehydrogenase, presumably involved in detoxification metabolism, is used to point out a physiological function of MDH in the oxidation of D-mannitol with high specificity and fluxional efficiency under prevailing reaction conditions in vivo [6].
  • Glucitol phosphorylation assays in which soluble and membrane fractions of cells grown on glucose (which did not synthesize the glucitol PTS) or cells grown on glucitol were used confirmed that there is a separate, soluble, glucitol-specific PTS component, which is the product of the gutB gene [7].
  • Expression of the gutB gene alone (encoding the glucitol enzyme III), subcloned downstream from either the lactose promoter or the tetracycline resistance promoter, inhibited utilization of the same compounds [8].
  • The resulting enzyme preparation catalyzed the oxidation of pentitols (L-arabinitol) and hexitols (D-allitol, D-sorbitol, L-iditol, L-mannitol) to the same corresponding ketoses as mammalian sorbitol dehydrogenase (SDH), albeit with different catalytic efficacies, showing highest k(cat)/K(m) for L-arabinitol [9].
  • The SLDH was active on mannitol, glycerol and other sugar alcohols as well as on D-sorbitol to produce respective keto-aldoses [3].

Chemical compound and disease context of gutB

  • For the development of the SLDH activity in E. coli, co-expression of the sldA and sldB genes and the presence of pyrrloquinolone quinone as a co-factor were required [4].
  • We purified a one-subunit-type SLDH (80 kDa) from the membrane fraction of Gluconobacter suboxydans IFO 3255 solubilized with Triton X-100 in the presence of D-sorbitol, but the cofactor could not be identified from the purified enzyme [3].

Biological context of gutB

  • The productivity of 2-KLGA was improved up to 9.8 mg/ml by changing to an expression system with two plasmids, pBBR-SDH-tufB (for SDH/SNDH) and pUCP19-SLDH (for SLDH), respectively [10].
  • After drug administration body temperature and rumen contractions were slightly depressed, whereas urea values gradually increased; serum sorbitol dehydrogenase (SDH) activity, plasma iron concentration and the number of circulating lymphocytes were significantly lower [11].

Associations of gutB with chemical compounds


Analytical, diagnostic and therapeutic context of gutB


  1. Molecular population genetics of Escherichia coli: DNA sequence diversity at the celC, crr, and gutB loci of natural isolates. Hall, B.G., Sharp, P.M. Mol. Biol. Evol. (1992) [Pubmed]
  2. Cloning and expression of glucose 3-dehydrogenase from Halomonas sp. alpha-15 in Escherichia coli. Kojima, K., Tsugawa, W., Sode, K. Biochem. Biophys. Res. Commun. (2001) [Pubmed]
  3. Membrane-bound D-sorbitol dehydrogenase of Gluconobacter suboxydans IFO 3255--enzymatic and genetic characterization. Hoshino, T., Sugisawa, T., Shinjoh, M., Tomiyama, N., Miyazaki, T. Biochim. Biophys. Acta (2003) [Pubmed]
  4. Molecular cloning and functional expression of D-sorbitol dehydrogenase from Gluconobacter suboxydans IF03255, which requires pyrroloquinoline quinone and hydrophobic protein SldB for activity development in E. coli. Miyazaki, T., Tomiyama, N., Shinjoh, M., Hoshino, T. Biosci. Biotechnol. Biochem. (2002) [Pubmed]
  5. Cloning and expression of the gene encoding catalytic subunit of thermostable glucose dehydrogenase from Burkholderia cepacia in Escherichia coli. Inose, K., Fujikawa, M., Yamazaki, T., Kojima, K., Sode, K. Biochim. Biophys. Acta (2003) [Pubmed]
  6. Kinetic study of the catalytic mechanism of mannitol dehydrogenase from Pseudomonas fluorescens. Slatner, M., Nidetzky, B., Kulbe, K.D. Biochemistry (1999) [Pubmed]
  7. A gene system for glucitol transport and metabolism in Clostridium beijerinckii NCIMB 8052. Tangney, M., Brehm, J.K., Minton, N.P., Mitchell, W.J. Appl. Environ. Microbiol. (1998) [Pubmed]
  8. Regulation of gluconeogenesis by the glucitol enzyme III of the phosphotransferase system in Escherichia coli. Yamada, M., Feucht, B.U., Saier, M.H. J. Bacteriol. (1987) [Pubmed]
  9. The metabolic role and evolution of L-arabinitol 4-dehydrogenase of Hypocrea jecorina. Pail, M., Peterbauer, T., Seiboth, B., Hametner, C., Druzhinina, I., Kubicek, C.P. Eur. J. Biochem. (2004) [Pubmed]
  10. Metabolic engineering study on the direct fermentation of 2-keto-L-gulonic acid, a key intermediate of L-ascorbic acid in Pseudomonas putida IFO3738. Shibata, T., Ichikawa, C., Matsuura, M., Takata, Y., Noguchi, Y., Saito, Y., Yamashita, M. J. Biosci. Bioeng. (2000) [Pubmed]
  11. Endotoxin-induced fever and associated haematological and blood biochemical changes in the goat: the effect of repeated administration and the influence of flurbiprofen. van Miert, A.S., van Duin, C.T., Verheijden, J.H., Schotman, A.J. Res. Vet. Sci. (1982) [Pubmed]
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